{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:46:59Z","timestamp":1776016019160,"version":"3.50.1"},"posted":{"date-parts":[[2025,6,25]]},"group-title":"Neuroscience","reference-count":47,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01-EY034493-01"],"award-info":[{"award-number":["R01-EY034493-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Retinal degenerative diseases are a major cause of blindness in humans that often result in permanent and progressive loss of vision. Unlike humans, zebrafish possess the remarkable ability to regenerate lost retinal neurons through M\u00fcller glia (MG) reprogramming and asymmetric cell division to produce multipotent retinal progenitor cells (RPCs). While most studies on the molecular mechanisms underlying this regeneration process have focused on intracellular mechanisms, the role of the microenvironment surrounding retinal cells, the extracellular matrix (ECM), has been understudied. Laminins are heterotrimeric glycoproteins, are principal components of the ECM basement membrane, and play important roles in vertebrate retinal development. Here, we examine the role of \u03b21b chain-containing laminins in the regenerative response of the zebrafish retina. We found that the zebrafish\n                  <jats:italic>lamb1b<\/jats:italic>\n                  gene is differentially expressed during MG reprogramming and MG and NPC proliferation during retinal regeneration. Further, we found that \u03b21b-containing laminins play important roles in regulating MG and NPC proliferation and neuroprotection of photoreceptors in light-damaged zebrafish retinas. Finally, Lam\u03b21b plays an important role in regulating the expression of integrin receptors and other laminin genes during the regeneration response. Taken together, Lam\u03b21b, and likely other ECM components, play a critical role in the MG-dependent neuronal regeneration response in the zebrafish retina.\n                <\/jats:p>","DOI":"10.1101\/2025.06.24.661136","type":"posted-content","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T16:00:18Z","timestamp":1750867218000},"source":"Crossref","is-referenced-by-count":0,"title":["Regulation of Adult Zebrafish Retinal Regeneration by Lam\u03b21b-Chain-Containing Laminins"],"prefix":"10.64898","author":[{"given":"Dmitri","family":"Serjanov","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Center for Stem Cells and Regenerative Medicine, and Center for Zebrafish Research, Galvin Life Sciences Building, University of Notre Dame, Notre Dame, IN, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Twist","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Center for Stem Cells and Regenerative Medicine, and Center for Zebrafish Research, Galvin Life Sciences Building, University of Notre Dame, Notre Dame, IN, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoyuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Center for Stem Cells and Regenerative Medicine, and Center for Zebrafish Research, Galvin Life Sciences Building, University of Notre Dame, Notre Dame, IN, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-4403","authenticated-orcid":false,"given":"David R.","family":"Hyde","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Center for Stem Cells and Regenerative Medicine, and Center for Zebrafish Research, Galvin Life Sciences Building, University of Notre Dame, Notre Dame, IN, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041210090366000_2025.06.24.661136v1.1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1624-07.2007"},{"key":"2026041210090366000_2025.06.24.661136v1.2","first-page":"775","article-title":"Gene expression profiles of intact and regenerating zebrafish retina","volume":"11","year":"2005","journal-title":"Mol. 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