{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:51:25Z","timestamp":1782280285461,"version":"3.54.5"},"posted":{"date-parts":[[2025,7,2]]},"group-title":"Developmental Biology","reference-count":71,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The self-renewal and differentiation of germline stem cells (GSCs) are tightly regulated during oogenesis. The\n                  <jats:italic>Drosophila<\/jats:italic>\n                  female germline provides a powerful model to study these regulatory mechanisms. We previously identified Sakura (also known as Bourbon\/CG14545) as a crucial factor for maintenance and differentiation of GSCs and oogenesis, and demonstrated that Sakura binds to Ovarian Tumor (Otu), another essential regulator of these processes. Here, we identify MYCBP (c-Myc binding protein) as an additional essential component of this regulatory network. We show that MYCBP physically associates with itself, Sakura, and Otu, forming binary and ternary complexes including a MYCBP\u2022Sakura\u2022Otu complex. MYCBP is highly expressed in the ovary, and\n                  <jats:italic>mycbp<\/jats:italic>\n                  null mutant females exhibit rudimentary ovaries with germline-less and tumorous ovarioles, fail to produce eggs, and are completely sterile. Germline-specific depletion of\n                  <jats:italic>mycbp<\/jats:italic>\n                  disrupts Dpp\/BMP signaling, causing aberrant expression of\n                  <jats:italic>bag-of-marbles<\/jats:italic>\n                  (\n                  <jats:italic>bam<\/jats:italic>\n                  ) and leading to defective differentiation and GSC loss. In addition,\n                  <jats:italic>mycbp<\/jats:italic>\n                  is required for female-specific splicing of\n                  <jats:italic>sex-lethal<\/jats:italic>\n                  (\n                  <jats:italic>sxl<\/jats:italic>\n                  ), a master regulator of sex identity determination. These phenotypes closely resemble those observed those of\n                  <jats:italic>sakura<\/jats:italic>\n                  and\n                  <jats:italic>otu<\/jats:italic>\n                  mutants. Together, our findings reveal that MYCBP functions in concert with Sakura and Otu to coordinate self-renewal and differentiation of GSCs and oogenesis in\n                  <jats:italic>Drosophila<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.1101\/2025.07.01.662550","type":"posted-content","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T17:15:11Z","timestamp":1751476511000},"source":"Crossref","is-referenced-by-count":0,"title":["MYCBP interacts with Sakura and Otu and is essential for germline stem cell renewal and differentiation and oogenesis"],"prefix":"10.64898","author":[{"given":"Azali","family":"Azlan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5814-8206","authenticated-orcid":false,"given":"Ryuya","family":"Fukunaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025070400150760000_2025.07.01.662550v1.1","doi-asserted-by":"crossref","unstructured":"Azlan A , Zhu L , Fukunaga R. 2024. Female-germline specific protein Sakura interacts with Otu and is crucial for germline stem cell renewal and differentiation and oogenesis. bioRxiv.","DOI":"10.7554\/eLife.103828.1"},{"key":"2025070400150760000_2025.07.01.662550v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90157-T"},{"key":"2025070400150760000_2025.07.01.662550v1.3","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.3.797"},{"key":"2025070400150760000_2025.07.01.662550v1.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120473109"},{"key":"2025070400150760000_2025.07.01.662550v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.09.033"},{"key":"2025070400150760000_2025.07.01.662550v1.6","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00325"},{"key":"2025070400150760000_2025.07.01.662550v1.7","doi-asserted-by":"publisher","DOI":"10.1242\/dev.039032"},{"key":"2025070400150760000_2025.07.01.662550v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.01.027"},{"key":"2025070400150760000_2025.07.01.662550v1.9","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.23.3715"},{"key":"2025070400150760000_2025.07.01.662550v1.10","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056242"},{"key":"2025070400150760000_2025.07.01.662550v1.11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015874108"},{"key":"2025070400150760000_2025.07.01.662550v1.12","doi-asserted-by":"crossref","unstructured":"ElMaghraby MF , Tirian L , Senti KA , Meixner K , Brennecke J . 2022. 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