{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:32:19Z","timestamp":1785429139308,"version":"3.56.0"},"posted":{"date-parts":[[2025,7,2]]},"group-title":"Microbiology","reference-count":104,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["R01AI189516"],"award-info":[{"award-number":["R01AI189516"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Pioneer in Research Award"],"award-info":[{"award-number":["Pioneer in Research Award"]}],"id":[{"id":"https:\/\/ror.org\/041ee2w62","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                  is a pathogen of concern and a leading cause of multidrug-resistant healthcare-associated infections. The\n                  <jats:italic>A. baumannii<\/jats:italic>\n                  outer membrane is a barrier to antimicrobials and host defenses but must also allow essential nutrients to permeate. Here, we investigate the functional importance of the putative DcaP-family outer membrane porins, which include a proposed vaccine target in\n                  <jats:italic>A. baumannii<\/jats:italic>\n                  . All\n                  <jats:italic>A. baumannii<\/jats:italic>\n                  genomes surveyed encode multiple DcaP family porins, which we classify in four classes based on protein sequence phylogeny (DcaP1-4). DcaP proteins encoded by species in other genera could not be mapped to these DcaP classes and phylogenetic analysis suggests DcaP1-4 proteins diversified within\n                  <jats:italic>Acinetobacter<\/jats:italic>\n                  . Phenotypic array assays and additional experiments show that the DcaP proteins were necessary for growth on multiple di- and tri-carboxylic acids as sole carbon sources, including citric acid and tricarballylic acid. Specifically, DcaP3 was required; however, DcaP1, DcaP2, and DcaP4 were all able to complement the total\n                  <jats:italic>dcaP<\/jats:italic>\n                  knockout to varying degrees, suggesting partial functional overlap. Finally, a mutant lacking all DcaP proteins was attenuated in the liver and spleen in a mouse model of bloodstream infection and complemented by expression of DcaP3. However, a \u0394\n                  <jats:italic>dcaP3<\/jats:italic>\n                  mutant had no defect, demonstrating functional redundancy among DcaP proteins during infection. These findings provide insight on how\n                  <jats:italic>A. baumannii<\/jats:italic>\n                  acquires nutrients through the outer membrane barrier and show DcaP proteins are important during infection in specific host niches, validating their potential as a therapeutic target.\n                <\/jats:p>","DOI":"10.1101\/2025.07.02.662759","type":"posted-content","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T01:00:10Z","timestamp":1751504410000},"source":"Crossref","is-referenced-by-count":0,"title":["DcaP-Family Porins are Required for Carboxylic Acid Utilization and Infection in\n                  <i>Acinetobacter baumannii<\/i>"],"prefix":"10.64898","author":[{"given":"Hannah R.","family":"Noel","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois Chicago, Chicago, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita R.","family":"Kesav","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois Chicago, Chicago, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan D.","family":"Winkelman","sequence":"additional","affiliation":[{"name":"Trestle LLC, Milwaukee, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7458-6129","authenticated-orcid":false,"given":"Lauren D.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois Chicago, Chicago, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026040308450556000_2025.07.02.662759v3.1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000337"},{"key":"2026040308450556000_2025.07.02.662759v3.2","unstructured":"World Health Organization. No time to wait: securing the future from drug-resistant infections [Report to the Secretary-General of the United Nations]. World Health Organization and Interagency Coordination Group on Antimicrobial Resistance; 2019 Apr. Report No."},{"key":"2026040308450556000_2025.07.02.662759v3.3","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2016.174"},{"key":"2026040308450556000_2025.07.02.662759v3.4","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"2026040308450556000_2025.07.02.662759v3.5","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000414"},{"key":"2026040308450556000_2025.07.02.662759v3.6","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.593-656.2003"},{"key":"2026040308450556000_2025.07.02.662759v3.7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-115714"},{"key":"2026040308450556000_2025.07.02.662759v3.8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062160"},{"key":"2026040308450556000_2025.07.02.662759v3.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107833"},{"key":"2026040308450556000_2025.07.02.662759v3.10","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-032421-111116"},{"key":"2026040308450556000_2025.07.02.662759v3.11","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00435-12"},{"key":"2026040308450556000_2025.07.02.662759v3.12","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.7b00168"},{"key":"2026040308450556000_2025.07.02.662759v3.13","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06645-0"},{"key":"2026040308450556000_2025.07.02.662759v3.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2016.10.009"},{"key":"2026040308450556000_2025.07.02.662759v3.15","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-188425"},{"key":"2026040308450556000_2025.07.02.662759v3.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.10.063"},{"key":"2026040308450556000_2025.07.02.662759v3.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2015.12.009"},{"key":"2026040308450556000_2025.07.02.662759v3.18","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-4-1213"},{"key":"2026040308450556000_2025.07.02.662759v3.19","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.1.524-532.2003"},{"key":"2026040308450556000_2025.07.02.662759v3.20","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4817-4827.2001"},{"key":"2026040308450556000_2025.07.02.662759v3.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2018.10.021"},{"key":"2026040308450556000_2025.07.02.662759v3.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2021.105346"},{"key":"2026040308450556000_2025.07.02.662759v3.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105882"},{"key":"2026040308450556000_2025.07.02.662759v3.24","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-021-10316-7"},{"key":"2026040308450556000_2025.07.02.662759v3.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104114"},{"key":"2026040308450556000_2025.07.02.662759v3.26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190599"},{"key":"2026040308450556000_2025.07.02.662759v3.27","doi-asserted-by":"publisher","DOI":"10.1021\/pr101299j"},{"key":"2026040308450556000_2025.07.02.662759v3.28","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.75"},{"key":"2026040308450556000_2025.07.02.662759v3.29","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"2026040308450556000_2025.07.02.662759v3.30","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae909"},{"key":"2026040308450556000_2025.07.02.662759v3.31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.12.014"},{"key":"2026040308450556000_2025.07.02.662759v3.32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.00987.x"},{"key":"2026040308450556000_2025.07.02.662759v3.33","doi-asserted-by":"publisher","DOI":"10.1128\/aem.02111-23"},{"key":"2026040308450556000_2025.07.02.662759v3.34","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001570"},{"key":"2026040308450556000_2025.07.02.662759v3.35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky603"},{"key":"2026040308450556000_2025.07.02.662759v3.36","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01076-14"},{"key":"2026040308450556000_2025.07.02.662759v3.37","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00433-22"},{"key":"2026040308450556000_2025.07.02.662759v3.38","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuae019"},{"key":"2026040308450556000_2025.07.02.662759v3.39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072968"},{"key":"2026040308450556000_2025.07.02.662759v3.40","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-009-0158-y"},{"key":"2026040308450556000_2025.07.02.662759v3.41","doi-asserted-by":"publisher","DOI":"10.1021\/pr400603f"},{"key":"2026040308450556000_2025.07.02.662759v3.42","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01294-22"},{"key":"2026040308450556000_2025.07.02.662759v3.43","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.4.1432-1440.2005"},{"key":"2026040308450556000_2025.07.02.662759v3.44","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr267"},{"key":"2026040308450556000_2025.07.02.662759v3.45","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.32.100178.002025"},{"key":"2026040308450556000_2025.07.02.662759v3.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.03.016"},{"key":"2026040308450556000_2025.07.02.662759v3.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2023.102707"},{"key":"2026040308450556000_2025.07.02.662759v3.48","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000765"},{"key":"2026040308450556000_2025.07.02.662759v3.49","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001010"},{"key":"2026040308450556000_2025.07.02.662759v3.50","doi-asserted-by":"publisher","DOI":"10.1042\/bj1030342"},{"key":"2026040308450556000_2025.07.02.662759v3.51","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.4.643"},{"key":"2026040308450556000_2025.07.02.662759v3.52","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2000.00357.x"},{"key":"2026040308450556000_2025.07.02.662759v3.53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803151105"},{"key":"2026040308450556000_2025.07.02.662759v3.54","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.1.45-54.2006"},{"key":"2026040308450556000_2025.07.02.662759v3.55","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01296-06"},{"key":"2026040308450556000_2025.07.02.662759v3.56","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-265983"},{"key":"2026040308450556000_2025.07.02.662759v3.57","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4114"},{"key":"2026040308450556000_2025.07.02.662759v3.58","doi-asserted-by":"publisher","DOI":"10.1038\/nature18597"},{"key":"2026040308450556000_2025.07.02.662759v3.59","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058628"},{"key":"2026040308450556000_2025.07.02.662759v3.60","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.5.1336"},{"key":"2026040308450556000_2025.07.02.662759v3.61","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/23.2.329"},{"key":"2026040308450556000_2025.07.02.662759v3.62","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11101297"},{"key":"2026040308450556000_2025.07.02.662759v3.63","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00392-19"},{"key":"2026040308450556000_2025.07.02.662759v3.64","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.667776"},{"key":"2026040308450556000_2025.07.02.662759v3.65","doi-asserted-by":"publisher","DOI":"10.1101\/2024.10.19.619093"},{"key":"2026040308450556000_2025.07.02.662759v3.66","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2009.01.001"},{"key":"2026040308450556000_2025.07.02.662759v3.67","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.6.1629-1637.2004"},{"key":"2026040308450556000_2025.07.02.662759v3.68","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012425"},{"key":"2026040308450556000_2025.07.02.662759v3.69","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00746-18"},{"key":"2026040308450556000_2025.07.02.662759v3.70","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008010"},{"key":"2026040308450556000_2025.07.02.662759v3.71","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10080957"},{"key":"2026040308450556000_2025.07.02.662759v3.72","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00096-09"},{"key":"2026040308450556000_2025.07.02.662759v3.73","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-216"},{"key":"2026040308450556000_2025.07.02.662759v3.74","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01041.x"},{"key":"2026040308450556000_2025.07.02.662759v3.75","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2020.1855912"},{"key":"2026040308450556000_2025.07.02.662759v3.76","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00454-21"},{"key":"2026040308450556000_2025.07.02.662759v3.77","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00131-15"},{"key":"2026040308450556000_2025.07.02.662759v3.78","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00130-9"},{"key":"2026040308450556000_2025.07.02.662759v3.79","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"2026040308450556000_2025.07.02.662759v3.80","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059388"},{"key":"2026040308450556000_2025.07.02.662759v3.81","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.07.002"},{"key":"2026040308450556000_2025.07.02.662759v3.82","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00188-15"},{"key":"2026040308450556000_2025.07.02.662759v3.83","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0151-5"},{"key":"2026040308450556000_2025.07.02.662759v3.84","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1832-y"},{"key":"2026040308450556000_2025.07.02.662759v3.85","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02804-23"},{"key":"2026040308450556000_2025.07.02.662759v3.86","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"2026040308450556000_2025.07.02.662759v3.87","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0721-2"},{"key":"2026040308450556000_2025.07.02.662759v3.88","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl529"},{"key":"2026040308450556000_2025.07.02.662759v3.89","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4229"},{"key":"2026040308450556000_2025.07.02.662759v3.90","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2018.10.021"},{"key":"2026040308450556000_2025.07.02.662759v3.91","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1010"},{"key":"2026040308450556000_2025.07.02.662759v3.92","doi-asserted-by":"publisher","DOI":"10.1128\/br.21.3.195-213.1957"},{"key":"2026040308450556000_2025.07.02.662759v3.93","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.75"},{"key":"2026040308450556000_2025.07.02.662759v3.94","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2018.10.021"},{"key":"2026040308450556000_2025.07.02.662759v3.95","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1010"},{"key":"2026040308450556000_2025.07.02.662759v3.96","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/j.chom.2025.07.003","article-title":"Amino acid competition shapes Acinetobacter baumannii gut carriage","volume":"33","year":"2025","journal-title":"Cell Host Microbe"},{"key":"2026040308450556000_2025.07.02.662759v3.97","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01076-14"},{"key":"2026040308450556000_2025.07.02.662759v3.98","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00131-15"},{"key":"2026040308450556000_2025.07.02.662759v3.99","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.4.1648"},{"key":"2026040308450556000_2025.07.02.662759v3.100","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth765"},{"key":"2026040308450556000_2025.07.02.662759v3.101","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00130-9"},{"key":"2026040308450556000_2025.07.02.662759v3.102","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"2026040308450556000_2025.07.02.662759v3.103","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059388"},{"key":"2026040308450556000_2025.07.02.662759v3.104","doi-asserted-by":"publisher","DOI":"10.2144\/99265bm05"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.07.02.662759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T15:45:14Z","timestamp":1775231114000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.07.02.662759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":104,"URL":"https:\/\/doi.org\/10.1101\/2025.07.02.662759","relation":{},"subject":[],"published":{"date-parts":[[2025,7,2]]},"subtype":"preprint"}}