{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:43:23Z","timestamp":1768524203361,"version":"3.49.0"},"posted":{"date-parts":[[2025,7,3]]},"group-title":"Microbiology","reference-count":88,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,7,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Biofilms are communities of bacteria growing within a matrix composed of polymeric substances that act as a barrier to antimicrobials, making bacteria within a biofilm recalcitrant to conventional antibiotic treatments. This lifestyle of bacteria is especially relevant during its pathogenesis, as observed in the case of\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  infections of immunocompromised patients. However, pico- to nanomolar concentrations of nitric oxide (NO) have been shown to be efficient in triggering\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  to disperse from biofilms, suggesting that a combination of NO exposure and antibiotic treatment may help in mitigating infections. In\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  , the NosP-NahK two component system, which comprises a NO sensing hemoprotein, NosP, and its associated hybrid histidine kinase, NahK, has been proven to be necessary for NO mediated biofilm dispersal. NahK also has additional roles in biofilm formation, motility, denitrification and virulence, due to its regulation of a global post-transcriptional regulator RsmA. Here, we uncover a novel role of NahK in enhancing the resistance of\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  to signaling concentrations of NO. Deletion of\n                  <jats:italic>nahK<\/jats:italic>\n                  sensitizes the strain to nanomolar levels of NO, resulting in DNA damage. Consequently, the SOS stress response pathway is induced, which causes phenotypes such as cell filamentation and cell clustering due to lysis-mediated release of extracellular DNA. Our data also indicate that increased susceptibility of the \u0394\n                  <jats:italic>nahK<\/jats:italic>\n                  strain to NO is due to the antagonization of RsmA, and is restricted to amino acid starved media, suggesting that NahK may also have previously unappreciated roles in modulating amino acid metabolism.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    infections of cystic fibrosis patients can result in increased risk of fatality, necessitating the development of better therapeutic strategies to treat its infections. Here, we present data suggesting that nanomolar concentrations of NO, that are normally used to disperse\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    from biofilms, can also sensitize the bacterium to NO mediated DNA damage upon loss of function of the histidine kinase NahK. The resulting SOS stress response may benefit the bacterium through favorable mutations but also poses a risk due to SOS-associated pyocin production through cell lysis. Hence, understanding the molecular mechanisms underlying NahK mediated resistance to NO is important to leverage this regulation and identify novel targets to treat\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    infections.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.07.03.662950","type":"posted-content","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:20:12Z","timestamp":1751588412000},"source":"Crossref","is-referenced-by-count":1,"title":["The histidine kinase NahK in\n                  <i>Pseudomonas aeruginosa<\/i>\n                  is essential for nitric oxide-stress resistance in nutrient starved media"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9755-0510","authenticated-orcid":false,"given":"Sweta","family":"Anantharaman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1465-1512","authenticated-orcid":false,"given":"Jiayuan","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1891-839X","authenticated-orcid":false,"given":"Elizabeth M.","family":"Boon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025070708150936000_2025.07.03.662950v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imj.2023.05.003"},{"key":"2025070708150936000_2025.07.03.662950v1.2","doi-asserted-by":"publisher","DOI":"10.1080\/1120009X.2020.1823679"},{"key":"2025070708150936000_2025.07.03.662950v1.3","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24948"},{"key":"2025070708150936000_2025.07.03.662950v1.4","doi-asserted-by":"crossref","unstructured":"Chung J , Eisha S , Park S , Morris AJ , Martin I . 2023. 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