{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T21:21:01Z","timestamp":1777411261320,"version":"3.51.4"},"posted":{"date-parts":[[2025,7,11]]},"group-title":"Neuroscience","reference-count":50,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH NIGMS","award":["R01GM082949"],"award-info":[{"award-number":["R01GM082949"]}]},{"name":"NINDS\/BRAIN Initiative","award":["U24NS120055"],"award-info":[{"award-number":["U24NS120055"]}]},{"name":"NSF","award":["2014862"],"award-info":[{"award-number":["2014862"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["509099330"],"award-info":[{"award-number":["509099330"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["Exc-2049-390688087"],"award-info":[{"award-number":["Exc-2049-390688087"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,7,14]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Neuronal function relies on the precise spatial organization of intracellular membrane-bounded organelles involved in anabolism and Ca\n                  <jats:sup>2+<\/jats:sup>\n                  sequestration, such as the Golgi apparatus, mitochondria and the endoplasmic reticulum (ER), along with structures involved in catabolism, such as lysosomes. Despite their known roles in energy supply, calcium homeostasis, and proteostasis, our understanding of how the anabolism-linked organelles are structurally arranged within neurons remains incomplete. Due to the tremendous complexity in the morphologies and fine structural features and interwoven nature of these intracellular organelles, particularly the ER, our understanding of their structural organization is limited, particularly, with regard to quantitative assessments of their sites of interaction and accurate measures of their volumetric proportions inside of a single large neuron. To approach this challenge, we used serial block-face scanning electron microscopy (SBEM) to generate large-scale 3D EM volumes and electron tomography on high-pressure frozen tissue of the rodent cerebellum, including the largest cells in the vertebrate brain, the cerebellar Purkinje neuron as well as the most abundant cell type in the vertebrate brain, the much smaller cerebellar granule neuron. We reconstructed the neuronal ultrastructure of these different cell types, focusing on the ER, mitochondria and membrane contact sites, to then characterize intracellular motifs and organization principles in detail, providing a first full map to quantitatively describe a neuronal\n                  <jats:italic>endoarchitectome<\/jats:italic>\n                  . At the gross level organization, we found that the intracellular composite of organelles are cell type specific features, with specific differences between Purkinje neurons and Granule cells. At the level of fine structure, we mapped ultrastructural domains within Purkinje neurons where ER and mitochondria associate directly. In addition to cell type specific differences, we observed significant subcellular regional variation, particularly within the axon initial segment (AIS) of Purkinje neurons, where we identified ultrastructural domains with sharply contrasting distributions of ER and mitochondria. These findings suggest a finely tuned spatial organization of organelles that may underpin the distinct functional demands along the axon. We expect that our subcellular map, along with the methods developed to obtain these maps, will facilitate future studies in health, aging and disease to characterize defined features, by developing a framework for quantitative analysis of the neuronal ultrastructure.\n                <\/jats:p>","DOI":"10.1101\/2025.07.08.663701","type":"posted-content","created":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T06:55:15Z","timestamp":1752303315000},"source":"Crossref","is-referenced-by-count":1,"title":["Deep mapping of the\n                  <i>endomembrane system<\/i>\n                  of cerebellar Purkinje neurons"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4961-7832","authenticated-orcid":false,"given":"Matthias G","family":"Haberl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3722-1072","authenticated-orcid":false,"given":"Silvia","family":"Viana da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastien","family":"Phan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Bushong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Deerinck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8893-8455","authenticated-orcid":false,"given":"Mark H","family":"Ellisman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025071615150705000_2025.07.08.663701v2.1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.1.3.257"},{"key":"2025071615150705000_2025.07.08.663701v2.2","doi-asserted-by":"publisher","DOI":"10.1126\/science.725607"},{"key":"2025071615150705000_2025.07.08.663701v2.3","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-12-03135.1985"},{"key":"2025071615150705000_2025.07.08.663701v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.01.010"},{"key":"2025071615150705000_2025.07.08.663701v2.5","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-11-04636.1993"},{"key":"2025071615150705000_2025.07.08.663701v2.6","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-01-00190.1997"},{"key":"2025071615150705000_2025.07.08.663701v2.7","first-page":"3111","article-title":"The neuronal endomembrane system","volume":"5","year":"1985","journal-title":"I. 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