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Microglia were the most perturbed cell type at both stages, showing increased oxidative stress and polarization toward both pro- and anti-inflammatory phenotypes at PND5. Ligand-receptor and regulon analysis revealed a shift from reparative IGF2 and TGF-\u03b2 signaling at PND2 to proinflammatory Wnt signaling and activation of Runx1 and Stat5 at PND5. These findings highlight the importance of developmental timing in shaping the neuroimmune response to IVH and identify potential stage-specific therapeutic targets.<\/jats:p>","DOI":"10.1101\/2025.07.11.664369","type":"posted-content","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T13:10:21Z","timestamp":1752757821000},"source":"Crossref","is-referenced-by-count":0,"title":["Developmental Stage-Dependent Transcriptomic Responses to Neonatal Intraventricular Hemorrhage"],"prefix":"10.64898","author":[{"given":"Elizabeth","family":"Wallace-Anthony","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6331-6733","authenticated-orcid":false,"given":"Miriam","family":"Zamorano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5945-5235","authenticated-orcid":false,"given":"Hemendra J","family":"Vekaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4249-5502","authenticated-orcid":false,"given":"Braden B","family":"Oldham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiara P","family":"Umpornpun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8032-3755","authenticated-orcid":false,"given":"Scott D","family":"Olson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9028-9458","authenticated-orcid":false,"given":"Stefano","family":"Berto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon A","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"11","key":"2025071910450735000_2025.07.11.664369v1.1","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1038\/s41593-024-01769-2","article-title":"Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain","volume":"27","year":"2024","journal-title":"Nat Neurosci"},{"key":"2025071910450735000_2025.07.11.664369v1.2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00228"},{"key":"2025071910450735000_2025.07.11.664369v1.3","doi-asserted-by":"crossref","unstructured":"Agarwal A , Zamorano M , Collier A , Oldham B , Riggs-Harpur K , Nguyen TL , et al. 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