{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:12:21Z","timestamp":1788862341608,"version":"build-2803163510"},"posted":{"date-parts":[[2025,7,31]]},"group-title":"Neuroscience","reference-count":47,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R01MH128217"],"award-info":[{"award-number":["R01MH128217"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01MH137047"],"award-info":[{"award-number":["R01MH137047"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Science Foundation","award":["DGE-1650441"],"award-info":[{"award-number":["DGE-1650441"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Psilocybin has profound therapeutic potential for various mental health disorders, but its mechanisms of action are unknown. Functional MRI studies have reported the effects of psilocybin on brain activity and connectivity; however, these measurements rely on neurovascular coupling to infer neural activity changes and assume that blood flow responses to neural activity are not altered by psilocybin. Using two-photon excited fluorescence imaging in the visual cortex of awake mice to simultaneously measure neural activity and capillary blood flow dynamics, we found that psilocybin administration prolonged the increase in visual stimulus-evoked capillary blood flow \u2013 an effect which was reduced by pretreatment with a 5-HT\n                  <jats:sub>2A<\/jats:sub>\n                  R antagonist \u2013 despite not causing changes in the stimulus-evoked neural response. Multi-modal widefield imaging also showed that psilocybin extends the stimulus-evoked vascular responses in surface vessels with no observed effect on the population neural response. Computational simulation with a whole-brain neural mass model showed that prolonged neurovascular coupling responses can lead to spurious increases in BOLD-based measures of functional connectivity. Together, these findings demonstrate that psilocybin broadens neurovascular responses in the brain and highlights the importance of accounting for these effects when interpreting human neuroimaging data of psychedelic drug action.\n                <\/jats:p>","DOI":"10.1101\/2025.07.25.666803","type":"posted-content","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T19:00:22Z","timestamp":1753988422000},"source":"Crossref","is-referenced-by-count":4,"title":["Psilocybin Prolongs the Neurovascular Coupling Response in Mouse Visual Cortex"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1744-0785","authenticated-orcid":false,"given":"Rick T.","family":"Zirkel","sequence":"first","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8797-0090","authenticated-orcid":false,"given":"Matthew","family":"Isaacson","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Liao","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"},{"name":"Interdepartmental Neuroscience Program, Yale University, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Yi","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keri","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4558-7402","authenticated-orcid":false,"given":"Daniel","family":"Rivera","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5050-8342","authenticated-orcid":false,"given":"Amy","family":"Kuceyeski","sequence":"additional","affiliation":[{"name":"Department of Radiology, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomi","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2169-1667","authenticated-orcid":false,"given":"Alex C.","family":"Kwan","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"},{"name":"Department of Psychiatry, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7800-9596","authenticated-orcid":false,"given":"Chris B.","family":"Schaffer","sequence":"additional","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026032804500920000_2025.07.25.666803v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2017.84"},{"key":"2026032804500920000_2025.07.25.666803v2.2","first-page":"959","volume":"31","year":"2020","journal-title":"Classic psychedelics as therapeutics for psychiatric disorders"},{"key":"2026032804500920000_2025.07.25.666803v2.3","doi-asserted-by":"publisher","DOI":"10.1038\/S41583-020-0367-2"},{"key":"2026032804500920000_2025.07.25.666803v2.4","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1176\/appi.ajp.20230172","article-title":"Psychedelics as Transformative Therapeutics","volume":"180","year":"2023","journal-title":"Am J Psychiatry"},{"key":"2026032804500920000_2025.07.25.666803v2.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119598109"},{"key":"2026032804500920000_2025.07.25.666803v2.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01744-z"},{"key":"2026032804500920000_2025.07.25.666803v2.7","first-page":"947622","article-title":"Altered brain activity and functional connectivity after MDMA-assisted therapy for post-traumatic stress disorder","volume":"13","year":"2022","journal-title":"Front Psychiatry"},{"key":"2026032804500920000_2025.07.25.666803v2.8","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.35082"},{"key":"2026032804500920000_2025.07.25.666803v2.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07624-5"},{"key":"2026032804500920000_2025.07.25.666803v2.10","doi-asserted-by":"crossref","unstructured":"Whitten, L. 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