{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:28:31Z","timestamp":1785421711539,"version":"3.56.0"},"posted":{"date-parts":[[2025,8,1]]},"group-title":"Immunology","reference-count":0,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["#P30CA016059"],"award-info":[{"award-number":["#P30CA016059"]}],"id":[{"id":"https:\/\/ror.org\/040gcmg81","id-type":"ROR","asserted-by":"publisher"}]},{"name":"ADA Science & Research Institute","award":["Volpe Research Scholar Award"],"award-info":[{"award-number":["Volpe Research Scholar Award"]}]},{"name":"Philips Institute for Oral Health Research","award":["Startup Funds"],"award-info":[{"award-number":["Startup Funds"]}]},{"award":["Large Research Grant"],"award-info":[{"award-number":["Large Research Grant"]}],"id":[{"id":"https:\/\/ror.org\/054xkse79","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Center for Gastrointestinal Biology and Disease","award":["CGIBD P30 DK034987"],"award-info":[{"award-number":["CGIBD P30 DK034987"]}]},{"name":"UNC Nutrition Obesity Research Center","award":["NORC P30 DK056350"],"award-info":[{"award-number":["NORC P30 DK056350"]}]},{"name":"UPenn Oral Maxillofacial Surgery Department","award":["Schoenleber Grant"],"award-info":[{"award-number":["Schoenleber Grant"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["#2021\/11082-4"],"award-info":[{"award-number":["#2021\/11082-4"]}]},{"award":["2022 Overseas Researcher Grant"],"award-info":[{"award-number":["2022 Overseas Researcher Grant"]}],"id":[{"id":"https:\/\/ror.org\/05jk51a88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["#1R03DE034507-01"],"award-info":[{"award-number":["#1R03DE034507-01"]}],"id":[{"id":"https:\/\/ror.org\/004a2wv92","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Next-Gen Researcher Pilot Award"],"award-info":[{"award-number":["Next-Gen Researcher Pilot Award"]}],"id":[{"id":"https:\/\/ror.org\/02qenvm24","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,8,22]]},"abstract":"<jats:p>Oral inflammatory diseases affect nearly half of the global population. Among them, newly defined peri-implantitis and high-grade periodontitis represent rapidly advancing inflammatory disease types, marked by relatively rapid tissue destruction. Despite their prevalence, the cell mechanisms and spatial architecture driving this severity remain poorly understood. Focusing first on peri-implantitis versus low- and moderate-grade periodontitis, we applied microbial profiling, single-cell RNA sequencing (scRNA-seq), and spatial proteomics (sp-proteomics) to uncover shared pathogenic programs linked to accelerated niche breakdown. Furthermore, to preserve spatial fidelity, each tissue was anatomically orientated along the tooth- or implant-epithelial interface, analogous sites of disease origination. Laser capture microdissection followed by microbiome analysis of unique tissue compartments revealed reduced bacterial load and diversity in peri-implantitis stroma. We then expanded our version-1 Human Periodontal Atlas by integrating newly generated peri-implantitis scRNAseq data (36-total samples; 121395-cells), revealing widespread transcriptional alterations, including oxidative stress, hypoxic, and NAD+ metabolism-associated signatures, primarily in a subpopulation of TNFRSF6B+\/ICAM1+ post-capillary venules. We then performed high-resolution sp-proteomics (15-total samples; 337260-cells) and analyzed VEC states and associated neighborhoods via AstroSuite using newly developed tri-wise spatial analysis. This revealed CD34+-VEC loss and CD38+-VEC expansion almost exclusively in peri-implantitis. We extended this analysis to high-grade periodontitis. Mucosal biopsies from four lesion-affected and four unaffected sites within the same individuals (1:1 matched; 8 samples; 225137-cells) again demonstrated spatially restricted CD38+-VEC remodeling exclusively in affected tissues, with similar vasculopathy front patterning. The findings nominate spatially distinct vasculopathy patterning as a hallmark of rapidly advancing oral inflammation and a targetable therapeutic axis.<\/jats:p>","DOI":"10.1101\/2025.07.29.667333","type":"posted-content","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T21:10:21Z","timestamp":1754082621000},"source":"Crossref","is-referenced-by-count":2,"title":["CD38\u207a Endothelial Remodeling Defines Spatially Diverse Vasculopathy Programs in Rapidly Advancing Oral Inflammation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8018-0405","authenticated-orcid":false,"given":"Quinn T","family":"Easter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5374-6111","authenticated-orcid":false,"given":"Khoa","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5125-2326","authenticated-orcid":false,"given":"Camila","family":"Stolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0560-7982","authenticated-orcid":false,"given":"Jialiu","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2132-3402","authenticated-orcid":false,"given":"Bruno","family":"Matuck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-2076","authenticated-orcid":false,"given":"Akira","family":"Hasuike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6581-3139","authenticated-orcid":false,"given":"Zabdiel","family":"Alvarado-Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8276-2206","authenticated-orcid":false,"given":"Zhaoxu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7702-6178","authenticated-orcid":false,"given":"Apoena","family":"Aguiar Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivedita","family":"Pareek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0325-1289","authenticated-orcid":false,"given":"Andrea M","family":"Azcarate-Peril","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8331-2357","authenticated-orcid":false,"given":"Di","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1743-5855","authenticated-orcid":false,"given":"Renato","family":"Casarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2967-8846","authenticated-orcid":false,"given":"Kang I","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0555-9412","authenticated-orcid":false,"given":"Jinze","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5565-0524","authenticated-orcid":false,"given":"Kevin M","family":"Byrd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.07.29.667333","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:15:26Z","timestamp":1768468526000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.07.29.667333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1101\/2025.07.29.667333","relation":{},"subject":[],"published":{"date-parts":[[2025,8,1]]},"subtype":"preprint"}}