{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:52:55Z","timestamp":1782280375036,"version":"3.54.5"},"posted":{"date-parts":[[2025,7,31]]},"group-title":"Developmental Biology","reference-count":199,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"crossref","award":["EY032532"],"award-info":[{"award-number":["EY032532"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"crossref","award":["EY020481"],"award-info":[{"award-number":["EY020481"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"crossref","award":["EY035381"],"award-info":[{"award-number":["EY035381"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"crossref"}]},{"name":"State University of New York College of Optometry","award":["EIP"],"award-info":[{"award-number":["EIP"]}]},{"name":"DAAD"},{"name":"F\u00f6rderverein Augenoptik \/Optometrie der Berliner Hochschule f\u00fcr Technik e.V"},{"name":"SUNY Graduate Assistantship"},{"name":"WashU Department of Ophthalmology and Visual Sciences from Research to Prevent Blindness"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  microRNAs (miRNAs) play a pivotal role during the early phases of retinal development, but their impact on late-phase retinogenesis is unknown. We depleted miRNAs in late retinal progenitor\/precursor cells (RPCs\/PCs) via a conditional Dicer knock-out. Optical coherence tomography (OCT), electroretinography (ERG), histological, and transcriptional analyses were conducted in young and adult mice. Alterations in gene expression of late-born cells were observed as early as postnatal day 7 (P7), resulting in impaired rod function, a significantly reduced number of rod bipolar cells and their associated function, and a decreased M\u00fcller glia population at adult age. These defects appear to be caused by a delay in differentiation\/ incomplete maturation, as indicated by an enlarged progenitor\/precursor population at young ages that persists into adulthood. Notably, an increased population of HuC\/D+ amacrine cells was found. Luciferase assays led us to speculate that this increase may be due to the absence of\n                  <jats:italic>Elavl3<\/jats:italic>\n                  suppression via RPC-miRNAs. This suggests that Dicer\/miRNAs in late RPC\/PCs are essential for the proper formation and maturation of late RPC progenies and may also play a role in regulating cell state.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Graphical abstract<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"635135v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Summary statement<\/jats:title>\n                  <jats:p>Late-retinal progenitor microRNAs are essential for proper postnatal retinogenesis and retinal function.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.07.30.635135","type":"posted-content","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T20:35:14Z","timestamp":1753994114000},"source":"Crossref","is-referenced-by-count":1,"title":["Dicer is essential for proper maturation, composition, and function in the postnatal retina"],"prefix":"10.64898","author":[{"given":"Seoyoung","family":"Kang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Larbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eik","family":"Bruns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Hahne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Khodadadi-Jamayran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaitra","family":"Sreenivasaiah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Lima","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khulan","family":"Batsuuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Jager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Viswanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian S.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2559-7678","authenticated-orcid":false,"given":"Stefanie G.","family":"Wohl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025080117350694000_2025.07.30.635135v1.1","first-page":"235","article-title":"MicroRNAs as Neuronal Fate Determinants","volume":"20","year":"2013","journal-title":"Neuroscientist"},{"key":"2025080117350694000_2025.07.30.635135v1.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508214103"},{"key":"2025080117350694000_2025.07.30.635135v1.3","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0881"},{"key":"2025080117350694000_2025.07.30.635135v1.4","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.1038\/s41598-018-38294-9","article-title":"Conditional Dicer1 depletion using Chrnb4-Cre leads to cone cell death and impaired photopic vision","volume":"9","year":"2019","journal-title":"Sci Rep"},{"key":"2025080117350694000_2025.07.30.635135v1.5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/cne.20387","article-title":"Comparison of the expression patterns of five neural RNA binding proteins in the Xenopus retina","volume":"481","year":"2005","journal-title":"J Comp Neurol"},{"key":"2025080117350694000_2025.07.30.635135v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.04.003"},{"key":"2025080117350694000_2025.07.30.635135v1.7","doi-asserted-by":"crossref","unstructured":"Amini-Farsani, Z. & Asgharzade, S . 2019. The impact of mir-183\/182\/96 gene regulation on the maturation, survival, and function of photoreceptor cells in the retina. J Comp Neurol.","DOI":"10.1002\/cne.24833"},{"key":"2025080117350694000_2025.07.30.635135v1.8","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"2025080117350694000_2025.07.30.635135v1.9","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20161"},{"key":"2025080117350694000_2025.07.30.635135v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00062-3"},{"key":"2025080117350694000_2025.07.30.635135v1.11","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s40135-014-0048-2","article-title":"Development of Retinal Amacrine Cells and Their Dendritic Stratification","volume":"2","year":"2014","journal-title":"Curr Ophthalmol Rep"},{"key":"2025080117350694000_2025.07.30.635135v1.12","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21449"},{"key":"2025080117350694000_2025.07.30.635135v1.13","first-page":"1293","article-title":"Pax-6, Prox 1, and Chx10 homeobox gene expression correlates with phenotypic fate of retinal precursor cells","volume":"38","year":"1997","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2025080117350694000_2025.07.30.635135v1.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1624-07.2007"},{"key":"2025080117350694000_2025.07.30.635135v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2013.08.002"},{"key":"2025080117350694000_2025.07.30.635135v1.16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020247"},{"key":"2025080117350694000_2025.07.30.635135v1.17","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902210103"},{"key":"2025080117350694000_2025.07.30.635135v1.18","doi-asserted-by":"publisher","DOI":"10.1261\/rna.039164.113"},{"key":"2025080117350694000_2025.07.30.635135v1.19","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201300104"},{"key":"2025080117350694000_2025.07.30.635135v1.20","doi-asserted-by":"publisher","DOI":"10.1242\/dev.064006"},{"key":"2025080117350694000_2025.07.30.635135v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2011.02.002"},{"key":"2025080117350694000_2025.07.30.635135v1.22","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21458"},{"key":"2025080117350694000_2025.07.30.635135v1.23","doi-asserted-by":"publisher","DOI":"10.1038\/ng0496-376"},{"key":"2025080117350694000_2025.07.30.635135v1.24","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0969-x"},{"key":"2025080117350694000_2025.07.30.635135v1.25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405514200"},{"key":"2025080117350694000_2025.07.30.635135v1.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.2.589"},{"key":"2025080117350694000_2025.07.30.635135v1.27","doi-asserted-by":"publisher","DOI":"10.1038\/nature09092"},{"key":"2025080117350694000_2025.07.30.635135v1.28","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1953310"},{"key":"2025080117350694000_2025.07.30.635135v1.29","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEURON.2019.04.010"},{"key":"2025080117350694000_2025.07.30.635135v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/0013-4694(52)90086-2"},{"key":"2025080117350694000_2025.07.30.635135v1.31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914785107"},{"key":"2025080117350694000_2025.07.30.635135v1.32","doi-asserted-by":"publisher","DOI":"10.1002\/bies.10356"},{"key":"2025080117350694000_2025.07.30.635135v1.33","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00220"},{"key":"2025080117350694000_2025.07.30.635135v1.34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.5483\/BMBRep.2009.42.3.131","article-title":"The role of microRNAs in synaptic development and function","volume":"42","year":"2009","journal-title":"Bmb Rep"},{"key":"2025080117350694000_2025.07.30.635135v1.35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0828-08.2008"},{"key":"2025080117350694000_2025.07.30.635135v1.36","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1749-8104-4-15","article-title":"Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells","volume":"4","year":"2009","journal-title":"Neural Dev"},{"key":"2025080117350694000_2025.07.30.635135v1.37","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23782"},{"key":"2025080117350694000_2025.07.30.635135v1.38","doi-asserted-by":"publisher","DOI":"10.1242\/dev.053637"},{"key":"2025080117350694000_2025.07.30.635135v1.39","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4815-07.2008"},{"key":"2025080117350694000_2025.07.30.635135v1.40","doi-asserted-by":"publisher","DOI":"10.1242\/dev.025080"},{"key":"2025080117350694000_2025.07.30.635135v1.41","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.082646sd"},{"key":"2025080117350694000_2025.07.30.635135v1.42","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0428"},{"key":"2025080117350694000_2025.07.30.635135v1.43","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2025080117350694000_2025.07.30.635135v1.44","doi-asserted-by":"crossref","unstructured":"Dupacova, N. , Antosova, B. , Paces, J. & Kozmik, Z . 2021. Meis homeobox genes control progenitor competence in the retina. Proc Natl Acad Sci U S A, 118.","DOI":"10.1073\/pnas.2013136118"},{"key":"2025080117350694000_2025.07.30.635135v1.45","doi-asserted-by":"publisher","DOI":"10.1038\/78774"},{"key":"2025080117350694000_2025.07.30.635135v1.46","doi-asserted-by":"publisher","DOI":"10.1242\/DEV.127.16.3593"},{"key":"2025080117350694000_2025.07.30.635135v1.47","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-12-04259.2001"},{"key":"2025080117350694000_2025.07.30.635135v1.48","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20010122)429:4<601::AID-CNE7>3.0.CO;2-V"},{"key":"2025080117350694000_2025.07.30.635135v1.49","doi-asserted-by":"publisher","DOI":"10.1038\/ng1144"},{"key":"2025080117350694000_2025.07.30.635135v1.50","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2520"},{"key":"2025080117350694000_2025.07.30.635135v1.51","doi-asserted-by":"publisher","DOI":"10.1007\/BF01203279"},{"key":"2025080117350694000_2025.07.30.635135v1.52","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-51703-x"},{"key":"2025080117350694000_2025.07.30.635135v1.53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111203"},{"key":"2025080117350694000_2025.07.30.635135v1.54","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202916"},{"key":"2025080117350694000_2025.07.30.635135v1.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.11.016"},{"key":"2025080117350694000_2025.07.30.635135v1.56","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02598"},{"key":"2025080117350694000_2025.07.30.635135v1.57","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81171-X"},{"key":"2025080117350694000_2025.07.30.635135v1.58","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22370"},{"key":"2025080117350694000_2025.07.30.635135v1.59","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr148"},{"key":"2025080117350694000_2025.07.30.635135v1.60","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21144"},{"key":"2025080117350694000_2025.07.30.635135v1.61","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-172"},{"key":"2025080117350694000_2025.07.30.635135v1.62","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4982-09.2010"},{"key":"2025080117350694000_2025.07.30.635135v1.63","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.133.4.1710"},{"key":"2025080117350694000_2025.07.30.635135v1.64","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx601"},{"key":"2025080117350694000_2025.07.30.635135v1.65","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3723"},{"key":"2025080117350694000_2025.07.30.635135v1.66","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4557"},{"key":"2025080117350694000_2025.07.30.635135v1.67","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00275"},{"key":"2025080117350694000_2025.07.30.635135v1.68","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903010308"},{"key":"2025080117350694000_2025.07.30.635135v1.69","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-7823-3_12"},{"key":"2025080117350694000_2025.07.30.635135v1.70","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90262-3"},{"key":"2025080117350694000_2025.07.30.635135v1.71","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.03.007"},{"key":"2025080117350694000_2025.07.30.635135v1.72","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-4657"},{"key":"2025080117350694000_2025.07.30.635135v1.73","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06638-9"},{"key":"2025080117350694000_2025.07.30.635135v1.74","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504834102"},{"key":"2025080117350694000_2025.07.30.635135v1.75","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1379"},{"key":"2025080117350694000_2025.07.30.635135v1.76","doi-asserted-by":"crossref","unstructured":"Hoang, T. , Wang, J. , Boyd, P. , Wang, F. , Santiago, C. , Jiang, L. , Yoo, S. , Lahne, M. , Todd, L. J. , Jia, M. , Saez, C. , Keuthan, C. , Palazzo, I. , Squires, N. , Campbell, W. A. , Rajaii, F. , Parayil, T. , Trinh, V. , Kim, D. W. , Wang, G. , Campbell, L. J. , Ash, J. , Fischer, A. J. , Hyde, D. R. , Qian, J. & Blackshaw, S . 2020. Gene regulatory networks controlling vertebrate retinal regeneration. Science, 370.","DOI":"10.1126\/science.abb8598"},{"key":"2025080117350694000_2025.07.30.635135v1.77","doi-asserted-by":"publisher","DOI":"10.1242\/DEV.127.12.2515"},{"key":"2025080117350694000_2025.07.30.635135v1.78","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6428"},{"key":"2025080117350694000_2025.07.30.635135v1.79","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2009.05.002"},{"key":"2025080117350694000_2025.07.30.635135v1.80","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-015-0118-x"},{"key":"2025080117350694000_2025.07.30.635135v1.81","doi-asserted-by":"crossref","first-page":"3565","DOI":"10.1007\/s12035-016-9899-x","article-title":"Transitional Progenitors during Vertebrate Retinogenesis","volume":"54","year":"2017","journal-title":"Mol Neurobiol"},{"key":"2025080117350694000_2025.07.30.635135v1.82","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21174"},{"key":"2025080117350694000_2025.07.30.635135v1.83","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(97)89968-2"},{"key":"2025080117350694000_2025.07.30.635135v1.84","doi-asserted-by":"crossref","unstructured":"Kang, S. & Wohl, S. G . 2022. Primary Cell Cultures to Study the Regeneration Potential of Murine Muller Glia after Microrna Treatment. J Vis Exp.","DOI":"10.3791\/63651"},{"key":"2025080117350694000_2025.07.30.635135v1.85","unstructured":"Karampelas, M. , Sim, D. A. & Keane, P. A . 2014. Spectral-domain Oct of the Rpe. Retinal Physician."},{"key":"2025080117350694000_2025.07.30.635135v1.86","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-23855-w"},{"key":"2025080117350694000_2025.07.30.635135v1.87","doi-asserted-by":"crossref","unstructured":"Kaufman, M. L. , Goodson, N. B. , Park, K. U. , Schwanke, M. , Office, E. , Schneider, S. R. , Abraham, J. , Hensley, A. , Jones, K. L. & Brzezinski, J. A . 2021. Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 activity. Development, 148.","DOI":"10.1242\/dev.199399"},{"key":"2025080117350694000_2025.07.30.635135v1.88","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.059659"},{"key":"2025080117350694000_2025.07.30.635135v1.89","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018472"},{"key":"2025080117350694000_2025.07.30.635135v1.90","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3178-07.2007"},{"key":"2025080117350694000_2025.07.30.635135v1.91","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.10.074"},{"key":"2025080117350694000_2025.07.30.635135v1.92","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1602532113"},{"key":"2025080117350694000_2025.07.30.635135v1.93","doi-asserted-by":"publisher","DOI":"10.1101\/gr.251421.119"},{"key":"2025080117350694000_2025.07.30.635135v1.94","first-page":"632632","article-title":"miRNAs and Muller Glia Reprogramming During Retina Regeneration","volume":"8","year":"2020","journal-title":"Front Cell Dev Biol"},{"key":"2025080117350694000_2025.07.30.635135v1.95","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(95)80063-8"},{"key":"2025080117350694000_2025.07.30.635135v1.96","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.cels.2018.08.011","article-title":"Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of mir-124 during Human Neurogenesis","volume":"7","year":"2018","journal-title":"Cell Syst"},{"key":"2025080117350694000_2025.07.30.635135v1.97","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1301837110"},{"key":"2025080117350694000_2025.07.30.635135v1.98","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70385-9"},{"key":"2025080117350694000_2025.07.30.635135v1.99","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00809-6"},{"key":"2025080117350694000_2025.07.30.635135v1.100","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1167\/iovs.66.3.7","article-title":"Dicer Loss in Muller Glia Leads to a Defined Sequence of Pathological Events Beginning With Cone Dysfunction","volume":"66","year":"2025","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2025080117350694000_2025.07.30.635135v1.101","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.23.11249"},{"key":"2025080117350694000_2025.07.30.635135v1.102","doi-asserted-by":"crossref","unstructured":"Le, N. , Vu, T. D. , Palazzo, I. , Pulya, R. , Kim, Y. , Blackshaw, S. & Hoang, T. 2023. Robust reprogramming of glia into neurons by inhibition of Notch signaling and Nfi factors in adult mammalian retina. bioRxiv.","DOI":"10.1101\/2023.10.29.560483"},{"key":"2025080117350694000_2025.07.30.635135v1.103","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90529-Y"},{"key":"2025080117350694000_2025.07.30.635135v1.104","doi-asserted-by":"crossref","unstructured":"Lee, S. H. , Won, Y. , Gibbs, D. , Caldwell, B. , Goldstein, A. , Choi, E. & Goldenring, J. R . 2024. Amphiregulin Switches Progenitor Cell Fate for Lineage Commitment During Gastric Mucosal Regeneration. Gastroenterology.","DOI":"10.1053\/j.gastro.2024.03.009"},{"key":"2025080117350694000_2025.07.30.635135v1.105","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.007"},{"key":"2025080117350694000_2025.07.30.635135v1.106","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.08.041"},{"key":"2025080117350694000_2025.07.30.635135v1.107","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2020.147265"},{"key":"2025080117350694000_2025.07.30.635135v1.108","doi-asserted-by":"crossref","unstructured":"Liao, F. , Liu, H. , Milla-Navarro, S. , Villa, P. & Germain, F . 2023. Origin of Retinal Oscillatory Potentials in the Mouse, a Tool to Specifically Locate Retinal Damage. Int J Mol Sci, 24.","DOI":"10.3390\/ijms24043126"},{"key":"2025080117350694000_2025.07.30.635135v1.109","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005435"},{"key":"2025080117350694000_2025.07.30.635135v1.110","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2025080117350694000_2025.07.30.635135v1.111","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040938"},{"key":"2025080117350694000_2025.07.30.635135v1.112","doi-asserted-by":"crossref","unstructured":"Lu, Y. L. , Liu, Y. , Mccoy, M. J. & Yoo, A. S . 2021. Mir-124 synergism with ELAVL3 enhances target gene expression to promote neuronal maturity. Proc Natl Acad Sci U S A, 118.","DOI":"10.1073\/pnas.2015454118"},{"key":"2025080117350694000_2025.07.30.635135v1.113","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.002"},{"key":"2025080117350694000_2025.07.30.635135v1.114","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00295-1"},{"key":"2025080117350694000_2025.07.30.635135v1.115","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)02028-2"},{"key":"2025080117350694000_2025.07.30.635135v1.116","doi-asserted-by":"crossref","unstructured":"Martin, M . 2011. Cutadapt removes adapter sequences from high-throughput sequencing reads. Emb.net.journal Bioinformatics in Action, 17.","DOI":"10.14806\/ej.17.1.200"},{"key":"2025080117350694000_2025.07.30.635135v1.117","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20064"},{"key":"2025080117350694000_2025.07.30.635135v1.118","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480250206"},{"key":"2025080117350694000_2025.07.30.635135v1.119","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEURON.2014.12.052"},{"key":"2025080117350694000_2025.07.30.635135v1.120","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-83395-7"},{"key":"2025080117350694000_2025.07.30.635135v1.121","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1803069115"},{"key":"2025080117350694000_2025.07.30.635135v1.122","unstructured":"Mcinnes, L. , Healy, J. & Melville, J. 2020.Umap: Uniform Manifold Approximation and Projection for Dimension Reduction. arXiv."},{"key":"2025080117350694000_2025.07.30.635135v1.123","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2012.08.009"},{"key":"2025080117350694000_2025.07.30.635135v1.124","doi-asserted-by":"publisher","DOI":"10.1038\/s41431-023-01456-z"},{"key":"2025080117350694000_2025.07.30.635135v1.125","first-page":"973140","article-title":"Bac-Dkk3-Egfp transgenic mouse: an in vivo analytical tool for Dkk3 expression","volume":"2012","year":"2012","journal-title":"J Biomed Biotechnol"},{"key":"2025080117350694000_2025.07.30.635135v1.126","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6989(91)90068-G"},{"key":"2025080117350694000_2025.07.30.635135v1.127","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022817"},{"key":"2025080117350694000_2025.07.30.635135v1.128","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121533"},{"key":"2025080117350694000_2025.07.30.635135v1.129","doi-asserted-by":"publisher","DOI":"10.1242\/dev.028308"},{"key":"2025080117350694000_2025.07.30.635135v1.130","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1097\/IAE.0000000000003974","article-title":"Retinal Optical Coherence Tomography Imaging Biomarkers: A Review of the Literature","volume":"44","year":"2024","journal-title":"Retina"},{"key":"2025080117350694000_2025.07.30.635135v1.131","doi-asserted-by":"crossref","unstructured":"Park, S. J. , Lei, W. , Pisano, J. , Orpia, A. , Minehart, J. , Pottackal, J. , Hanke-Gogokhia, C. , Zapadka, T. E. , Clarkson-Paredes, C. , Popratiloff, A. , Ross, S. E. , Singer, J. H. & Demb, J. B. 2023. Molecular identification of wide-field amacrine cells in mouse retina that encode stimulus orientation. bioRxiv.","DOI":"10.1101\/2023.12.28.573580"},{"key":"2025080117350694000_2025.07.30.635135v1.132","doi-asserted-by":"crossref","unstructured":"Perez-Leon, J. A. , Espinal-Centeno, A. , Mendoza-Gonzalez, Z. , Camacho, A. C. , Lopez, A. B. , Perez, R. A. & Miranda, M . 2022. Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina. Med Res Arch, 10.","DOI":"10.18103\/mra.v10i1.2624"},{"key":"2025080117350694000_2025.07.30.635135v1.133","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.02.029"},{"key":"2025080117350694000_2025.07.30.635135v1.134","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00056-8"},{"key":"2025080117350694000_2025.07.30.635135v1.135","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10175"},{"key":"2025080117350694000_2025.07.30.635135v1.136","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010021"},{"key":"2025080117350694000_2025.07.30.635135v1.137","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21746"},{"key":"2025080117350694000_2025.07.30.635135v1.138","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1991.tb00053.x"},{"key":"2025080117350694000_2025.07.30.635135v1.139","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0541"},{"key":"2025080117350694000_2025.07.30.635135v1.140","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"2025080117350694000_2025.07.30.635135v1.141","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq033"},{"key":"2025080117350694000_2025.07.30.635135v1.142","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24188"},{"key":"2025080117350694000_2025.07.30.635135v1.143","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2115"},{"key":"2025080117350694000_2025.07.30.635135v1.144","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107220108"},{"key":"2025080117350694000_2025.07.30.635135v1.145","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20134"},{"key":"2025080117350694000_2025.07.30.635135v1.146","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.09.030"},{"key":"2025080117350694000_2025.07.30.635135v1.147","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku376"},{"key":"2025080117350694000_2025.07.30.635135v1.148","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21730"},{"key":"2025080117350694000_2025.07.30.635135v1.149","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.03.039"},{"key":"2025080117350694000_2025.07.30.635135v1.150","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0736-5748(01)00051-X","article-title":"Constant dark-rearing effects on visual adaptation of the zebrafish Erg","volume":"19","year":"2001","journal-title":"Int J Dev Neurosci"},{"key":"2025080117350694000_2025.07.30.635135v1.151","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20318"},{"key":"2025080117350694000_2025.07.30.635135v1.152","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00159-8"},{"key":"2025080117350694000_2025.07.30.635135v1.153","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2001.1040"},{"key":"2025080117350694000_2025.07.30.635135v1.154","doi-asserted-by":"publisher","DOI":"10.1007\/BF01371129"},{"key":"2025080117350694000_2025.07.30.635135v1.155","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.07.054"},{"key":"2025080117350694000_2025.07.30.635135v1.156","doi-asserted-by":"publisher","DOI":"10.4161\/rna.21998"},{"key":"2025080117350694000_2025.07.30.635135v1.157","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1152\/jn.1986.56.4.1049","article-title":"Scotopic threshold response of proximal retina in cat","volume":"56","year":"1986","journal-title":"J Neurophysiol"},{"key":"2025080117350694000_2025.07.30.635135v1.158","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.02.055"},{"key":"2025080117350694000_2025.07.30.635135v1.159","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.12.041"},{"key":"2025080117350694000_2025.07.30.635135v1.160","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.omtn.2020.03.014","article-title":". circhmcu Promotes Proliferation and Metastasis of Breast Cancer by Sponging the let-7 Family","volume":"20","year":"2020","journal-title":"Mol Ther Nucleic Acids"},{"key":"2025080117350694000_2025.07.30.635135v1.161","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0206239"},{"key":"2025080117350694000_2025.07.30.635135v1.162","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.12.007"},{"key":"2025080117350694000_2025.07.30.635135v1.163","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-0976-7"},{"key":"2025080117350694000_2025.07.30.635135v1.164","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.770024"},{"key":"2025080117350694000_2025.07.30.635135v1.165","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-254292"},{"key":"2025080117350694000_2025.07.30.635135v1.166","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2004.01.045"},{"key":"2025080117350694000_2025.07.30.635135v1.167","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad283"},{"key":"2025080117350694000_2025.07.30.635135v1.168","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80093-8"},{"key":"2025080117350694000_2025.07.30.635135v1.169","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"},{"key":"2025080117350694000_2025.07.30.635135v1.170","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001588"},{"key":"2025080117350694000_2025.07.30.635135v1.171","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1515\/med-2022-0499","article-title":"Let-7i-3p inhibits the cell cycle, proliferation, invasion, and migration of colorectal cancer cells via downregulating CCND1","volume":"17","year":"2022","journal-title":"Open Med (Wars"},{"key":"2025080117350694000_2025.07.30.635135v1.172","doi-asserted-by":"publisher","DOI":"10.1038\/328131a0"},{"key":"2025080117350694000_2025.07.30.635135v1.173","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1510595112"},{"key":"2025080117350694000_2025.07.30.635135v1.174","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2010.04.002"},{"key":"2025080117350694000_2025.07.30.635135v1.175","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(98)00006-8"},{"key":"2025080117350694000_2025.07.30.635135v1.176","first-page":"1176","article-title":"The oscillatory potentials of the mudpuppy retina","volume":"17","year":"1978","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2025080117350694000_2025.07.30.635135v1.177","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1002\/kjm2.12236","article-title":"Let-7b downgrades CCND1 to repress osteogenic proliferation and differentiation of MC3T3-E1 cells: An implication in osteoporosis","volume":"36","year":"2020","journal-title":"Kaohsiung J Med Sci"},{"key":"2025080117350694000_2025.07.30.635135v1.178","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051052"},{"key":"2025080117350694000_2025.07.30.635135v1.179","doi-asserted-by":"publisher","DOI":"10.1017\/S095252380001097X"},{"key":"2025080117350694000_2025.07.30.635135v1.180","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19980511)394:3<374::AID-CNE8>3.0.CO;2-Z"},{"key":"2025080117350694000_2025.07.30.635135v1.181","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0415-14.2014"},{"key":"2025080117350694000_2025.07.30.635135v1.182","doi-asserted-by":"publisher","DOI":"10.4161\/rna.8.6.17665"},{"key":"2025080117350694000_2025.07.30.635135v1.183","unstructured":"Wohl, S. G. 2024. M\u00fcller Glia Development. Reference Module in Neuroscience and Biobehavioral Psychology"},{"key":"2025080117350694000_2025.07.30.635135v1.184","doi-asserted-by":"crossref","unstructured":"Wohl, S. G. , Hooper, M. J. & Reh, T. A . 2019. MicroRNAs mir-25, let-7 and mir-124 regulate the neurogenic potential of Muller glia in mice. Development, 146.","DOI":"10.1242\/dev.179556"},{"key":"2025080117350694000_2025.07.30.635135v1.185","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01624-y"},{"key":"2025080117350694000_2025.07.30.635135v1.186","doi-asserted-by":"publisher","DOI":"10.1038\/srep35423"},{"key":"2025080117350694000_2025.07.30.635135v1.187","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.62.4.1"},{"key":"2025080117350694000_2025.07.30.635135v1.188","doi-asserted-by":"crossref","first-page":"167518","DOI":"10.1016\/j.bbadis.2024.167518","article-title":"Role of Elavl-like Rna-binding protein in retinal development and signal transduction","volume":"1871","year":"2025","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"2025080117350694000_2025.07.30.635135v1.189","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1183-2"},{"key":"2025080117350694000_2025.07.30.635135v1.190","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0471-20.2020"},{"key":"2025080117350694000_2025.07.30.635135v1.191","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.07.024"},{"key":"2025080117350694000_2025.07.30.635135v1.192","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.08.078"},{"key":"2025080117350694000_2025.07.30.635135v1.193","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.08.100"},{"key":"2025080117350694000_2025.07.30.635135v1.194","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092120215"},{"key":"2025080117350694000_2025.07.30.635135v1.195","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(85)90211-1"},{"key":"2025080117350694000_2025.07.30.635135v1.196","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.exer.2018.06.016","article-title":"Characterization of canonical Wnt signalling changes after induced disruption of Muller cell in murine retina","volume":"175","year":"2018","journal-title":"Exp Eye Res"},{"key":"2025080117350694000_2025.07.30.635135v1.197","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.gep.2012.12.001","article-title":"The expression of NOTCH2, HES1 and SOX9 during mouse retinal development","volume":"13","year":"2013","journal-title":"Gene Expr Patterns"},{"key":"2025080117350694000_2025.07.30.635135v1.198","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129642"},{"key":"2025080117350694000_2025.07.30.635135v1.199","doi-asserted-by":"crossref","unstructured":"Zuzic, M. , Rojo Arias, J. E. , Wohl, S. G. & Busskamp, V . 2019. Retinal mirna Functions in Health and Disease. Genes (Basel), 10.","DOI":"10.3390\/genes10050377"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.07.30.635135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:01:57Z","timestamp":1782277317000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.07.30.635135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,31]]},"references-count":199,"URL":"https:\/\/doi.org\/10.1101\/2025.07.30.635135","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1016\/j.isci.2025.113794","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2025,7,31]]},"subtype":"preprint"}}