{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:25:19Z","timestamp":1768523119311,"version":"3.49.0"},"posted":{"date-parts":[[2025,7,31]]},"group-title":"Developmental Biology","reference-count":55,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH\/NIGMS","award":["P20 GM121316"],"award-info":[{"award-number":["P20 GM121316"]}]},{"name":"UNMC","award":["startup grant"],"award-info":[{"award-number":["startup grant"]}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["J1-3009 and J1-60013"],"award-info":[{"award-number":["J1-3009 and J1-60013"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["development funding pillar RSF-0106, and research core funding P1-0055"],"award-info":[{"award-number":["development funding pillar RSF-0106, and research core funding P1-0055"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007795","name":"Stowers Institute for Medical Research.","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007795","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,7,31]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>\n                  Animal tissue morphology is determined by the shape, position, and proliferative capacity of individual epithelial cells. Nevertheless, it remains incompletely understood how the dynamic shape transformations implicit in mitotic proliferation influence tissue packing, particularly at the level of basal cell contacts. Here, we use an\n                  <jats:italic>in silico<\/jats:italic>\n                  vertex model to show that epithelial mitotic rounding necessitates a sequence of dynamic basal contact rearrangements, including basal diminution of the mitotic cell volume, transient multicellular rosette assembly, basal reinsertion of daughter cells, and neighbor reorganization. We then leverage a mammalian intestinal organoid model to confirm nearly identical basal cell-contact dynamics as those predicted\n                  <jats:italic>in silico<\/jats:italic>\n                  . Pharmacological inhibition of mitotic progression reveals that two events\u2014basal diminution of the cell body and daughter cell reinsertion\u2014independently drive distinct contact rearrangements. Together, our results uncover a previously underappreciated topological role for basal mitotic cell dynamics in shaping epithelial packing and morphogenesis.\n                <\/jats:p>","DOI":"10.1101\/2025.07.30.665610","type":"posted-content","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T16:50:12Z","timestamp":1753980612000},"source":"Crossref","is-referenced-by-count":0,"title":["Basal Cell-Contact Dynamics Influence Tissue Packing in a Proliferating Mammalian Epithelium"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2876-0322","authenticated-orcid":false,"given":"Subramanian 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