{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:41:29Z","timestamp":1788932489674,"version":"build-2803163510"},"posted":{"date-parts":[[2025,8,20]]},"group-title":"Cell Biology","reference-count":70,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["1ZIADK075158"],"award-info":[{"award-number":["1ZIADK075158"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DAF2022-250616"],"award-info":[{"award-number":["DAF2022-250616"]}],"id":[{"id":"https:\/\/ror.org\/02qenvm24","id-type":"ROR","asserted-by":"publisher"}]},{"award":["09150172110086"],"award-info":[{"award-number":["09150172110086"]}],"id":[{"id":"https:\/\/ror.org\/04jsz6e67","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,8,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The lipids and proteins that comprise lipid droplets regulate several cellular functions including lipid storage, stress responses, and inflammation. Glial lipid droplets have been implicated in the pathogenesis and progression of Alzheimer\u2019s disease (AD), yet the mechanisms linking genetic risk to lipid droplet biology remain unclear. Here we examined how\n                  <jats:italic>APOE<\/jats:italic>\n                  , the strongest genetic modulator of late-onset AD, impacts lipid droplet composition and dynamics. We defined the lipid droplet-associated proteome and lipidome in human induced pluripotent stem cell-derived astrocytes harboring the three common\n                  <jats:italic>APOE<\/jats:italic>\n                  genotypes:\n                  <jats:italic>APOE2<\/jats:italic>\n                  (protective),\n                  <jats:italic>APOE3<\/jats:italic>\n                  (neutral), and\n                  <jats:italic>APOE4<\/jats:italic>\n                  (risk). Each\n                  <jats:italic>APOE<\/jats:italic>\n                  variant displays distinct lipid droplet-associated proteins and lipids. These molecular changes yield differences in lipophagy; lipid droplets in\n                  <jats:italic>APOE2<\/jats:italic>\n                  astrocytes undergo autophagic turnover, whereas those in\n                  <jats:italic>APOE4<\/jats:italic>\n                  astrocytes are resistant to degradation. These findings suggest that impaired lipid droplet clearance, rather than accumulation, distinguishes\n                  <jats:italic>APOE4<\/jats:italic>\n                  -associated AD risk, and may present a new metabolic node for modulating risk.\n                <\/jats:p>","DOI":"10.1101\/2025.08.19.669163","type":"posted-content","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T10:00:15Z","timestamp":1755684015000},"source":"Crossref","is-referenced-by-count":8,"title":["<i>APOE<\/i>\n                  genotypes differentially remodel the astrocytic lipid droplet-associated proteome to shape lipid droplet dynamics"],"prefix":"10.64898","author":[{"given":"Carla","family":"Cun\u00ed-L\u00f3pez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica T.","family":"Root","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Kowal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niek","family":"Blomberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Ghirlando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda G.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha J.","family":"Koppes-den Hertog","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Cookson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rik","family":"van der Kant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Giera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue Andy","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka S.","family":"Narayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025082112451209000_2025.08.19.669163v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0085-z"},{"key":"2025082112451209000_2025.08.19.669163v1.2","doi-asserted-by":"crossref","unstructured":"Welte, M. 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