{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T13:36:09Z","timestamp":1768570569028,"version":"3.49.0"},"posted":{"date-parts":[[2025,8,28]]},"group-title":"Epidemiology","reference-count":48,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,8,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Blood biomarkers can characterize the atrial substrate, helping to elucidate mechanisms of atrial fibrillation (AF) development. Understanding whether sedentary behavior affects AF-related biomarkers is key for future prevention strategies.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We studied 252 participants in PREDIMED-Plus, a multicenter randomized trial in Spain for the primary prevention of cardiovascular disease. A wrist-worn accelerometer was used to measure physical activity (PA) in a week at baseline and at least once during follow-up at years 3 and 5. Blood samples were collected at each time point to measure selected cardiovascular biomarkers: propeptide of procollagen type I, high-sensitivity (hs) troponin T (hsTnT), hs C-reactive protein, 3-nitrotyrosine, and N-terminal propeptide of B-type natriuretic peptide (NT-proBNP). Sedentary time was assessed as inactive time (&lt; 1.5 METs in waking time). Using isotemporal substitution, we analyzed the impact of replacing 30-min sedentary time per day with low-intensity PA (LPA, 1.5\u20133 METs), moderate to vigorous PA (MVPA, &gt; 3 METs) and time in bed (time difference between going to bed and getting up) on log-transformed biomarkers cross-sectionally and longitudinally, using linear regression and mixed models.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>At baseline, 252 eligible participants averaged 65 years of age (SD 4.9), and BMI 32.2 kg\/m\u00b2 (SD 3.3), and 40% were female. Cross-sectionally, replacing 30-min sedentary time with LPA, MVPA, or time in bed had no significant association with biomarkers. After 5 years, replacing baseline 30-min of sedentary time per day with LPA or MVPA was associated with lower hs-TnT concentrations compared to baseline (\u22124%, 95% CI - 8%, \u22121%; \u22122%, 95% CI \u22127%, 4%, respectively). Substituting 30-min sedentary time with LPA or MVPA showed nonsignificant reductions in NT-proBNP (\u22123%, 95% CI \u221211%, 5%; - 8%, 95% CI -20%, 5%, respectively), but not a consistent association with other biomarkers.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>In overweight\/obese individuals, prolonged sedentary time, when compared to engaging in PA, was associated with unfavorable changes of hs-TnT over 5 years, but no significant impact on other AF-related biomarkers.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.08.25.25334401","type":"posted-content","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T23:45:14Z","timestamp":1756424714000},"source":"Crossref","is-referenced-by-count":0,"title":["Association of sedentary behavior with atrial fibrillation-related biomarkers in a population with overweight or 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