{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:20:48Z","timestamp":1788510048774,"version":"build-2803163510"},"posted":{"date-parts":[[2025,9,1]]},"group-title":"Neuroscience","reference-count":53,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01DK128475"],"award-info":[{"award-number":["R01DK128475"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01DK139386"],"award-info":[{"award-number":["R01DK139386"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01DK140445"],"award-info":[{"award-number":["R01DK140445"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["K01 DK115634"],"award-info":[{"award-number":["K01 DK115634"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  End-organs such as the bladder rely on a delicate balance between internal urgency and voluntary restraint. However, the specific spinal circuits coordinating these functions remain poorly defined. Here, we identify a genetically defined population of lumbosacral spinal interneurons marked by\n                  <jats:italic>Trpc4<\/jats:italic>\n                  expression that gate bladder sensory input and regulate micturition reflexes. Single-cell transcriptomics and in situ physiology reveal molecularly distinct\n                  <jats:italic>Trpc4<\/jats:italic>\n                  \u207a subtypes. Functional manipulation reveals that\n                  <jats:italic>Trpc4<\/jats:italic>\n                  \u207a neurons are essential for coordinating bladder-sphincter activity and gating visceral pain. Their ablation leads to bladder hypersensitivity and voiding dysfunction, while targeted activation reverses these maladaptive states. Circuit tracing reveals convergence of primary afferent and descending brainstem inputs onto\n                  <jats:italic>Trpc4<\/jats:italic>\n                  \u207a neurons. These findings establish\n                  <jats:italic>Trpc4<\/jats:italic>\n                  \u207a interneurons are critical for bladder sensory-motor integration and extend classical spinal gating models to encompass visceral pain and organ reflex control.\n                <\/jats:p>","DOI":"10.1101\/2025.08.27.672386","type":"posted-content","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T15:50:12Z","timestamp":1756741812000},"source":"Crossref","is-referenced-by-count":1,"title":["A Spinal Circuit for Modular Gating of Organ Somatosensation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3306-4193","authenticated-orcid":false,"given":"Yufen","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2310-9008","authenticated-orcid":false,"given":"Fang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3647-6073","authenticated-orcid":false,"given":"Lite","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1851-7426","authenticated-orcid":false,"given":"Hannah J","family":"Hahm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique R","family":"Heitmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Okuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0303-5650","authenticated-orcid":false,"given":"Masahiro","family":"Kawatani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jahnia T","family":"Harrigan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica C","family":"Morrison-Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Petukhova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaotong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry A","family":"Heitmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9491-2793","authenticated-orcid":false,"given":"Vijay 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