{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:06:48Z","timestamp":1790014008561,"version":"4.0.1"},"posted":{"date-parts":[[2025,9,2]]},"group-title":"Developmental Biology","reference-count":54,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH","award":["R01GM150666"],"award-info":[{"award-number":["R01GM150666"]}]},{"name":"NIH","award":["R01HD119820"],"award-info":[{"award-number":["R01HD119820"]}]},{"name":"Shipley Foundation, Inc","award":["Stem Cell Award"],"award-info":[{"award-number":["Stem Cell Award"]}]},{"award":["Duke Science and Technology Spark Award"],"award-info":[{"award-number":["Duke Science and Technology Spark Award"]}],"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,17]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  The generation of a segmented body plan is a hallmark of vertebrate development. Biological oscillators provide a mechanism for timing the formation of repeated structures, yet to date only a few examples of signaling oscillators have been identified in development. Here, we show that the periodic addition of mineralizing segments in the zebrafish notochord is timed by tissue-wide oscillations of Erk activity. We report delayed oscillations in the transcription of\n                  <jats:italic>spry<\/jats:italic>\n                  and\n                  <jats:italic>dusp<\/jats:italic>\n                  genes, suggesting that they act as negative feedback. Erk activity is regulated by EGFR signaling and increased\n                  <jats:italic>egf<\/jats:italic>\n                  expression marks the emergence of oscillations via an infinite-period bifurcation, revealing the mechanism controlling the onset of notochord segmentation and the tunability of the oscillator. Our results show a remarkable degree of synchronization across the notochord and reveal an instance of biological clocks timing a patterning process, crucial for the development of the vertebral column from the notochord.\n                <\/jats:p>","DOI":"10.1101\/2025.08.28.672946","type":"posted-content","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T16:35:13Z","timestamp":1756830913000},"source":"Crossref","is-referenced-by-count":2,"title":["Tissue-wide Erk oscillations set the pace of zebrafish notochord segmentation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3067-7058","authenticated-orcid":false,"given":"Priyom","family":"Adhyapok","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 USA"},{"name":"Center for Quantitative Living Systems, Duke University Medical Center, Durham, NC 27710 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Norman","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Bagnat","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 USA"},{"name":"Center for Quantitative Living Systems, Duke University Medical Center, Durham, NC 27710 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9758-7925","authenticated-orcid":false,"given":"Stefano","family":"Di Talia","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 USA"},{"name":"Center for Quantitative Living Systems, Duke University Medical Center, Durham, NC 27710 USA"},{"name":"Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026092110100864000_2025.08.28.672946v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80451-1"},{"key":"2026092110100864000_2025.08.28.672946v2.2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3891"},{"key":"2026092110100864000_2025.08.28.672946v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(76)80131-2"},{"key":"2026092110100864000_2025.08.28.672946v2.4","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1038\/s41576-021-00355-7","article-title":"Towards a physical understanding of developmental patterning","volume":"22","year":"2021","journal-title":"Nat. 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