{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T01:46:30Z","timestamp":1768527990264,"version":"3.49.0"},"posted":{"date-parts":[[2025,9,4]]},"group-title":"Bioengineering","reference-count":56,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007927","name":"University of Oklahoma Health Sciences Center","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007927","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["OIA-2132161, 2238648, 2331409"],"award-info":[{"award-number":["OIA-2132161, 2238648, 2331409"]}]},{"name":"National Institute of Health","award":["R01DK133717"],"award-info":[{"award-number":["R01DK133717"]}]},{"DOI":"10.13039\/100008569","name":"Oklahoma Center for the Advancement of Science and Technology","doi-asserted-by":"crossref","award":["HR23-071"],"award-info":[{"award-number":["HR23-071"]}],"id":[{"id":"10.13039\/100008569","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Imaging COBRE","award":["P20 GM135009"],"award-info":[{"award-number":["P20 GM135009"]}]},{"DOI":"10.13039\/100001299","name":"the Prevent Cancer Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001299","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Data Institute for Societal Challenges and the Research Council funded by the Office of the Vice President for Research and Partnerships of the University of Oklahoma Norman Campus"},{"name":"Midwest Biomedical Accelerator Consortium"},{"name":"NIH Research Evaluation and Commercialization Hub"},{"name":"Financial support was provided by the OU LibrariesM-BM-^R Open Access Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Kidney transplantation remains the preferred treatment for patients with end-stage kidney disease. However, the ongoing shortage of donor organs continues to limit the availability of transplant treatments. Existing evaluation methods, such as the kidney donor profile index (KDPI) and pre-transplant donor biopsy (PTDB), have various limitations, including low discriminative power, invasiveness, and sampling errors, which reduce their effectiveness in organ quality assessment and contribute to the risk of unnecessary organ discard. In this study, we explored the dynamic optical coherence tomography (DOCT) as a real-time, non-invasive approach to monitor the viability of ex vivo mouse kidneys during static cold storage over 48 hours. The dynamic metrics logarithmic intensity variance (LIV), early OCT correlation decay speed (OCDS\n                  <jats:italic>\n                    <jats:sub>e<\/jats:sub>\n                  <\/jats:italic>\n                  ), and late OCT correlation decay speed (OCDS\n                  <jats:italic>\n                    <jats:sub>l<\/jats:sub>\n                  <\/jats:italic>\n                  ) were extracted from OCT signal fluctuations to quantify temporal and spatial tissue activity and deterioration. Our results demonstrate that DOCT provides complementary functional information to morphological assessment offered by conventional OCT imaging, showing potential to improve pre-transplant organ evaluation and clinic decision-making.\n                <\/jats:p>","DOI":"10.1101\/2025.08.30.673280","type":"posted-content","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T02:50:14Z","timestamp":1757040614000},"source":"Crossref","is-referenced-by-count":0,"title":["Real-Time Viability Assessment of Ex Vivo Mouse Kidneys for Transplant Applications Using Dynamic Optical Coherence Tomography"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3194-2546","authenticated-orcid":false,"given":"Ke","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trisha","family":"Valerio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cui","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronghao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tri","family":"Vu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bornface","family":"Mutembei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaustubh","family":"Pandit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio","family":"Silveyra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Duong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clint","family":"Hostetler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley","family":"Milam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradon","family":"Nave","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Squires","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongxin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kar-Ming","family":"Fung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narendra","family":"Battula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Potter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongle","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei R.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuye","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9499-5384","authenticated-orcid":false,"given":"Qinggong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025090815500818000_2025.08.30.673280v1.1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000006770"},{"key":"2025090815500818000_2025.08.30.673280v1.2","doi-asserted-by":"crossref","unstructured":"S. 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