{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:59:31Z","timestamp":1782280771465,"version":"3.54.5"},"posted":{"date-parts":[[2025,9,2]]},"group-title":"Cell Biology","reference-count":48,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 HL082792"],"award-info":[{"award-number":["R01 HL082792"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 GM137605"],"award-info":[{"award-number":["R01 GM137605"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R35 GM153257"],"award-info":[{"award-number":["R35 GM153257"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 AR084664"],"award-info":[{"award-number":["R01 AR084664"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["URoL2022048"],"award-info":[{"award-number":["URoL2022048"]}]},{"DOI":"10.13039\/501100001674","name":"Fondation Leducq","doi-asserted-by":"crossref","award":["20CVD01"],"award-info":[{"award-number":["20CVD01"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001674","name":"Fondation Leducq","doi-asserted-by":"crossref","award":["24CVD03"],"award-info":[{"award-number":["24CVD03"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001663","name":"Volkswagen Foundation","doi-asserted-by":"crossref","award":["A130142"],"award-info":[{"award-number":["A130142"]}],"id":[{"id":"10.13039\/501100001663","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Lamins form a dense meshwork at the inner surface of the inner nuclear membrane (INM), where they interact with other nuclear envelope proteins such as emerin. Emerin is an integral membrane protein that is part of the LEM (LAP2\/emerin\/MAN1) domain family, and mutations in either emerin or lamin A\/C can result in Emery-Dreifuss muscular dystrophy (EDMD) and other striated muscle diseases. Emerin is retained at the INM through direct interaction with lamin A\/C, and emerin\u2019s proper subcellular localization is critical for its ability to influence the mechanical properties of the nucleus and participate in various signaling processes. Nonetheless, the requirements for interaction between emerin and lamin A\/C at the INM remain incompletely understood. Here, we report that two distinct regions of lamin A\/C are each sufficient to properly localize emerin to the INM and prevent emerin\u2019s lateral diffusion within the INM. In addition to a previously described region of the lamin A\/C tail domain able to bind emerin, we identify a novel emerin-interacting domain comprising the linker between the rod and Ig-like fold domains of lamin A\/C. We further demonstrate that stably anchoring emerin to the INM requires assembly of A-type lamins into a filamentous network. Collectively, our findings suggest a revised model for emerin retention at the INM, which predicts that two independent lamin A\/C domains are required to retain emerin at the nuclear envelope.<\/jats:p>","DOI":"10.1101\/2025.09.01.673543","type":"posted-content","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:11Z","timestamp":1756857611000},"source":"Crossref","is-referenced-by-count":0,"title":["A-type lamins anchor emerin at the inner nuclear membrane via two independent binding sites"],"prefix":"10.64898","author":[{"given":"Jacob","family":"Odell","sequence":"first","affiliation":[{"name":"Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA"},{"name":"Graduate Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"Nedza","sequence":"additional","affiliation":[{"name":"Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA"},{"name":"Graduate Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4335-8611","authenticated-orcid":false,"given":"Jan","family":"Lammerding","sequence":"additional","affiliation":[{"name":"Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA"},{"name":"Graduate Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA"},{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026020606371967000_2025.09.01.673543v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/323560a0"},{"key":"2026020606371967000_2025.09.01.673543v2.2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.148247"},{"key":"2026020606371967000_2025.09.01.673543v2.3","doi-asserted-by":"publisher","DOI":"10.4161\/nucl.25751"},{"key":"2026020606371967000_2025.09.01.673543v2.4","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040844"},{"key":"2026020606371967000_2025.09.01.673543v2.5","doi-asserted-by":"publisher","DOI":"10.1038\/ng1294-323"},{"key":"2026020606371967000_2025.09.01.673543v2.6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"2026020606371967000_2025.09.01.673543v2.7","doi-asserted-by":"publisher","DOI":"10.1039\/c5sm00521c"},{"key":"2026020606371967000_2025.09.01.673543v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2023.102220"},{"key":"2026020606371967000_2025.09.01.673543v2.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812622115"},{"key":"2026020606371967000_2025.09.01.673543v2.10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2107770118"},{"key":"2026020606371967000_2025.09.01.673543v2.11","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.2023"},{"key":"2026020606371967000_2025.09.01.673543v2.12","doi-asserted-by":"publisher","DOI":"10.1126\/science.1084125"},{"key":"2026020606371967000_2025.09.01.673543v2.13","doi-asserted-by":"publisher","DOI":"10.1038\/nature15548"},{"key":"2026020606371967000_2025.09.01.673543v2.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114284"},{"key":"2026020606371967000_2025.09.01.673543v2.15","doi-asserted-by":"publisher","DOI":"10.1038\/nature01629"},{"key":"2026020606371967000_2025.09.01.673543v2.16","doi-asserted-by":"crossref","unstructured":"Florwick, A , Dharmaraj, T , Jurgens, J , Valle, D , and Wilson, KL (2017). 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