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However, the requirements for canonical polarity factors vary between tissues and organisms. For example, the basolateral protein lethal giant larvae (Lgl) is essential for epithelial polarity in\n                  <jats:italic>Drosophila<\/jats:italic>\n                  but dispensable in\n                  <jats:italic>C. elegans<\/jats:italic>\n                  . To better define the epithelial polarity program in\n                  <jats:italic>C. elegans<\/jats:italic>\n                  , we performed a genome-wide RNAi screen for synthetic lethality with\n                  <jats:italic>lgl-1<\/jats:italic>\n                  . Combined loss of LGL-1 and the RhoGAP PAC-1 caused embryonic lethality due to elongation defects and epidermal rupture. Epidermal cells showed expansion of the apical domain and aPKC, accompanied by mislocalization of junctional proteins and LET-413\n                  <jats:sup>Scribble<\/jats:sup>\n                  . These defects indicate overactivity of apical polarity determinants. Consistently, partial inactivation of aPKC or CDC-42 suppressed lethality in\n                  <jats:italic>pac-1; lgl-1<\/jats:italic>\n                  animals. Together, our results identify PAC-1 and LGL-1 as redundant inhibitors of apical polarity in the\n                  <jats:italic>C. elegans<\/jats:italic>\n                  embryonic epidermis and provide new insights into how conserved mechanisms are adapted across species.\n                <\/jats:p>","DOI":"10.1101\/2025.09.05.674236","type":"posted-content","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T20:10:12Z","timestamp":1757103012000},"source":"Crossref","is-referenced-by-count":0,"title":["LGL-1 and the RhoGAP protein PAC-1 redundantly polarize the\n                  <i>C. elegans<\/i>\n                  embryonic epidermis"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2184-3993","authenticated-orcid":false,"given":"Olga D.","family":"Jarosi\u0144ska","sequence":"first","affiliation":[{"name":"Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The 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