{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:39Z","timestamp":1792786179969,"version":"4.5.0"},"posted":{"date-parts":[[2025,9,13]]},"group-title":"Biochemistry","reference-count":78,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T00:00:00Z","timestamp":1757721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["DP2GM137419"],"award-info":[{"award-number":["DP2GM137419"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01DK137976"],"award-info":[{"award-number":["R01DK137976"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R35GM158265"],"award-info":[{"award-number":["R35GM158265"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01DK133479"],"award-info":[{"award-number":["R01DK133479"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Welch Foundation","award":["I-1911"],"award-info":[{"award-number":["I-1911"]}],"id":[{"id":"https:\/\/ror.org\/00np6vq88","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/006w34k90","id-type":"ROR","asserted-by":"publisher"}]},{"award":["A22068"],"award-info":[{"award-number":["A22068"]}],"id":[{"id":"https:\/\/ror.org\/02ebg5q27","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Hatch Grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Ether lipids play critical roles in membrane dynamics, antioxidant defense, and signaling. They comprise \u223c20% of mammalian phospholipids, and disruptions in their metabolism cause severe genetic disorders and are associated with neurodegenerative and metabolic diseases. Ether lipids are synthesized\n                  <jats:italic>de novo<\/jats:italic>\n                  from glycolytic intermediates or salvaged from the diet. While the products of these pathways are known, several key enzymes remain unidentified, including the 1-O-alkylglycerol kinase and the 1-O-alkyl-2-acetyl-\n                  <jats:italic>sn<\/jats:italic>\n                  -glycero-3-phosphate phosphatase. Here, we show that acyl-CoA dehydrogenase member 10 (ACAD10) catalyzes the phosphorylation of 1-O-alkylglycerols and the dephosphorylation of 1-O-alkyl-2-acetyl-\n                  <jats:italic>sn<\/jats:italic>\n                  -glycero-3-phosphate. Worms and mice lacking ACAD10 have reduced ether lipid levels and cannot salvage dietary alkylglycerols. Furthermore, individuals from the Akimel O\u2019odham (Pima) tribe carrying\n                  <jats:italic>ACAD10<\/jats:italic>\n                  polymorphisms also show decreased plasma ether lipid levels. Collectively, our findings resolve two long-standing gaps in ether lipid biochemistry and reveal a mechanistic link between ether lipid metabolism and a population-associated risk factor for type 2 diabetes.\n                <\/jats:p>","DOI":"10.1101\/2025.09.10.675409","type":"posted-content","created":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T08:00:12Z","timestamp":1757836812000},"source":"Crossref","is-referenced-by-count":0,"title":["ACAD10 encodes two orphan enzymes in the ether lipid biosynthetic and salvage pathways"],"prefix":"10.64898","author":[{"given":"James S.","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Purlyte","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor A.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jericha","family":"Mill","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lexus","family":"Tatge","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kizzar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Baldwin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edrees","family":"Rashan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0001-0087","authenticated-orcid":false,"given":"David J.","family":"Pagliarini","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri, 63110, USA."},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD, 20815, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana R.","family":"Tomchick","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."},{"name":"Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Paw\u0142owski","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD, 20815, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Douglas","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7350-6342","authenticated-orcid":false,"given":"Judith","family":"Simcox","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA."},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD, 20815, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9735-4678","authenticated-orcid":false,"given":"Vincent S.","family":"Tagliabracci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD, 20815, USA."},{"name":"Harold C. 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