{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T01:56:04Z","timestamp":1768528564333,"version":"3.49.0"},"posted":{"date-parts":[[2025,9,17]]},"group-title":"Neuroscience","reference-count":4,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100021054","name":"Military Operational Medicine Research Program","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100021054","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Introduction<\/jats:title>\n                  <jats:p>The purpose of the present study was to determine whether mild traumatic brain injury (mTBI+) results in latent changes in microRNA expression profiles across an episode of total sleep deprivation (TSD).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Seven previously concussed (mTBI+) adults (24.5 \u00b1 5.3 y.o.) and 6 non-concussed control adults (mTBI\u2212; 24.8 \u00b1 1.6 y.o.) underwent 24 h TSD (T2) preceded by 8 h baseline sleep (T1; BSL) and followed by 8 h recovery (T3; REC) sleep. Salivary microRNA expression was assessed across the entire study.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Subjects (mTBI+ and mTBI\u2212) had differential expression of salivary microRNA targets across TSD. mTBI+ subjects had greater change to microRNA expression profiles compared to mTBI\u2212 subjects between T1 and T3.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Discussion<\/jats:title>\n                  <jats:p>Although there is some evidence that TSD may unmask latent changes in gene expression in mTBI+ subjects, a definitive conclusion was precluded by differences in baseline sleep in mTBI+ (vs. mTBI\u2212) subjects measured through polysomnography (not shown). However, this study is unique in that the mTBI+ subjects were exposed to a sleep deprivation challenge very shortly after medical clearance from mTBI demonstrating lingering neurobiological impacts of mTBI.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.09.11.675463","type":"posted-content","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:10:13Z","timestamp":1758125413000},"source":"Crossref","is-referenced-by-count":0,"title":["Impact of &gt; 24 h sustained wakefulness and subsequent recovery sleep on time-dependent changes in microRNA factors of individuals with post-acute phase mild traumatic brain injury"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0821-8461","authenticated-orcid":false,"given":"Allison","family":"Brager","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katie","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassie","family":"Pattinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Peyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2465-4079","authenticated-orcid":false,"given":"Jessica","family":"Gill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025091906050491000_2025.09.11.675463v1.1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.13-0088"},{"key":"2025091906050491000_2025.09.11.675463v1.2","first-page":"879","article-title":"Sleep Disruptions among Returning Combat Veterans from Iraq and Afghanistan","volume":"176","year":"2010","journal-title":"Mil Med"},{"key":"2025091906050491000_2025.09.11.675463v1.3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3389\/fncir.2018.00014","article-title":"Sleep Deprivation and the Epigenome","volume":"12","year":"2018","journal-title":"Front Neural Circuits"},{"key":"2025091906050491000_2025.09.11.675463v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2015.05.014"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.09.11.675463","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:35:02Z","timestamp":1768469702000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.09.11.675463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,17]]},"references-count":4,"URL":"https:\/\/doi.org\/10.1101\/2025.09.11.675463","relation":{},"subject":[],"published":{"date-parts":[[2025,9,17]]},"subtype":"preprint"}}