{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:44:19Z","timestamp":1785437059370,"version":"3.56.0"},"posted":{"date-parts":[[2025,9,17]]},"group-title":"Developmental Biology","reference-count":46,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["5R01HD113569"],"award-info":[{"award-number":["5R01HD113569"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["HD30284"],"award-info":[{"award-number":["HD30284"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["T32HD098068"],"award-info":[{"award-number":["T32HD098068"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CA16672"],"award-info":[{"award-number":["CA16672"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["HD114881"],"award-info":[{"award-number":["HD114881"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2209150"],"award-info":[{"award-number":["2209150"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]},{"award":["1942250"],"award-info":[{"award-number":["1942250"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]},{"award":["695669"],"award-info":[{"award-number":["695669"]}],"id":[{"id":"https:\/\/ror.org\/0472cxd90","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/02gn6ta77","id-type":"ROR","asserted-by":"publisher"}]},{"name":"All Souls College, University of Oxford, GB"},{"award":["EP\/Y032756\/1"],"award-info":[{"award-number":["EP\/Y032756\/1"]}],"id":[{"id":"https:\/\/ror.org\/0439y7842","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/04twxam07","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>\n                    The brown anole is a model species of the genus\n                    <jats:italic>Anolis<\/jats:italic>\n                    , a squamate (encompassing lizards and snakes) group widely studied in evolutionary, behavioral, and developmental biology. Full genome annotation, the establishment of gene editing techniques, and comprehensive description of reproductive tract morphology and embryogenesis in this species, has laid the foundation for functional studies. However, analysis of brown anole oogenesis is still required and vital to optimize genome modification, mutant line establishment, and analyses of the evolution of reproductive developmental mechanisms.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    Here, we characterize ovary morphology and gametogenesis in the female brown anole,\n                    <jats:italic>A. sagrei<\/jats:italic>\n                    using brightfield imaging, microCT, histology staining, electron microscopy, and confocal imaging. We define 10 stages of oocyte maturation which commences inside the oogonial nest within the germinal bed and concludes with the mature follicle ready to ovulate based on follicle size, yolk-acquisition, and follicular, cellular, and basement membrane architecture.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We describe the complete oogenesis of the brown anole in 10 stages and report that oogenesis is highly conserved within iguanids, a suborder of lizards. With our staging framework, we lay the foundation for functional studies of oogenesis and optimized gene-editing.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.09.11.675729","type":"posted-content","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T17:25:29Z","timestamp":1758129929000},"source":"Crossref","is-referenced-by-count":0,"title":["Oogenesis and germinal bed morphology of the brown anole (\n                  <i>A. sagrei<\/i>\n                  )"],"prefix":"10.64898","author":[{"given":"Bonnie K.","family":"Kircher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8282-5695","authenticated-orcid":false,"given":"Antonia","family":"Weberling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin J.","family":"Vance","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia A.","family":"Shylo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Starr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7477-2384","authenticated-orcid":false,"given":"Zoe B.","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melainia","family":"McClain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1367-6312","authenticated-orcid":false,"given":"Florian","family":"Hollfelder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzannah A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Sanger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard R.","family":"Behringer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2774-3624","authenticated-orcid":false,"given":"Paul A.","family":"Trainor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"5","key":"2025091910050860000_2025.09.11.675729v1.1","doi-asserted-by":"crossref","first-page":"F783","DOI":"10.1152\/ajprenal.1987.253.5.F783","article-title":"Structure and development of the glomerular capillary wall and basement membrane","volume":"253","year":"1987","journal-title":"American Journal of Physiology-Renal Physiology"},{"key":"2025091910050860000_2025.09.11.675729v1.2","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20950"},{"key":"2025091910050860000_2025.09.11.675729v1.3","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1962.0019"},{"issue":"1","key":"2025091910050860000_2025.09.11.675729v1.4","doi-asserted-by":"crossref","first-page":"6763","DOI":"10.1038\/s41467-025-61441-6","article-title":"Basement membrane perforations guide anterior\u2013posterior axis formation","volume":"16","year":"2025","journal-title":"Nature Communications"},{"issue":"4","key":"2025091910050860000_2025.09.11.675729v1.5","first-page":"428","article-title":"The annotated anole: studies on control of lizard reproduction: anoline lizards make excellent experimental animals for analyzing reproduction at the physiological, behavioral, and species levels","volume":"65","year":"1977","journal-title":"American Scientist"},{"key":"2025091910050860000_2025.09.11.675729v1.6","first-page":"1","volume-title":"Advances in the Study of Behavior","volume":"11","year":"1980"},{"key":"2025091910050860000_2025.09.11.675729v1.7","doi-asserted-by":"publisher","DOI":"10.2108\/zs180030"},{"key":"2025091910050860000_2025.09.11.675729v1.8","doi-asserted-by":"crossref","unstructured":"Dekel, N . 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