{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:57:25Z","timestamp":1782280645743,"version":"3.54.5"},"posted":{"date-parts":[[2025,9,15]]},"group-title":"Developmental Biology","reference-count":21,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Metazoan eggs are surrounded by a specialized coat of extracellular matrix that mediates sperm-egg interactions. This coat is rapidly remodeled after fertilization to form a barrier that prevents polyspermy, protects against environmental insults, and provides structural support to the developing embryo. In\n                  <jats:italic>C. elegans<\/jats:italic>\n                  several oocyte surface proteins have been identified that mediate these events. However, whether two of these proteins, EGG-1 and EGG-2, are required for fertilization or downstream events has been unclear. Here, we address this question using more recent advances in genome editing tools through the creation of\n                  <jats:italic>egg-1 egg-2<\/jats:italic>\n                  deletions of the endogenous loci. We found that\n                  <jats:italic>egg-1 egg-2<\/jats:italic>\n                  oocytes are fertilization competent and form rudimentary eggshells. While the integrity of the\n                  <jats:italic>egg-1 egg-2<\/jats:italic>\n                  eggshells are compromised and often rupture within the uterus, surprisingly, some embryos are capable of undergoing several rounds of cell division. Overall, our findings demonstrate that EGG-1 and EGG-2 are not required for fertilization but are involved in post-fertilization processes.\n                <\/jats:p>","DOI":"10.1101\/2025.09.12.675948","type":"posted-content","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T21:05:50Z","timestamp":1757970350000},"source":"Crossref","is-referenced-by-count":0,"title":["Oocyte surface proteins EGG-1 and EGG-2 are required for eggshell integrity in\n                  <i>Caenorhabditis elegans<\/i>"],"prefix":"10.64898","author":[{"given":"Ji Kent","family":"Kwah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Pfeiffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mst Gitika","family":"Khanom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7697-8580","authenticated-orcid":false,"given":"Aimee","family":"Jaramillo-Lambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025091714350612000_2025.09.12.675948v1.1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.169730"},{"key":"2025091714350612000_2025.09.12.675948v1.2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.192997"},{"key":"2025091714350612000_2025.09.12.675948v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a071019"},{"key":"2025091714350612000_2025.09.12.675948v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.08.005"},{"key":"2025091714350612000_2025.09.12.675948v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90138-3"},{"key":"2025091714350612000_2025.09.12.675948v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.09.059"},{"key":"2025091714350612000_2025.09.12.675948v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.10.043"},{"key":"2025091714350612000_2025.09.12.675948v1.8","doi-asserted-by":"publisher","DOI":"10.3791\/65064"},{"key":"2025091714350612000_2025.09.12.675948v1.9","first-page":"2408","article-title":"Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C","volume":"15","year":"2001","journal-title":"elegans germ cell development. 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