{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:34:17Z","timestamp":1790667257226,"version":"4.1.0"},"posted":{"date-parts":[[2025,9,17]]},"group-title":"Developmental Biology","reference-count":93,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R00HD103778"],"award-info":[{"award-number":["R00HD103778"]}],"id":[{"id":"https:\/\/ror.org\/04byxyr05","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Boettcher Foundation Webb Waring Early Career Investigator"],"award-info":[{"award-number":["Boettcher Foundation Webb Waring Early Career Investigator"]}],"id":[{"id":"https:\/\/ror.org\/04byxyr05","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Mammalian ovary development is essential for female fertility, involving the complex spatial patterning of diverse cell types to establish the finite reserve of ovarian follicles. To uncover molecular mechanisms driving regionalization of the ovary while maintaining its native cellular architecture, we used Visium HD spatial transcriptomics. Our study captures all ovarian cell types at eight timepoints, generating a near single cell resolution library of spatial gene expression across development. Our analysis identified a previously uncharacterized \"medullary core\" domain that emerges by E16.5. Pre-granulosa cells (PGs) within this core express genes typically restricted to growing follicles in the postnatal ovary (\n                  <jats:italic>Nr5a2<\/jats:italic>\n                  ,\n                  <jats:italic>Slc18a2<\/jats:italic>\n                  ,\n                  <jats:italic>Ephx2)<\/jats:italic>\n                  suggesting core PGs are poised for activation while still in the fetal environment. This study provides a comprehensive architectural and molecular blueprint of the ovary, representing a fundamental resource for the investigation of regulatory mechanisms driving spatial patterning of the ovary and opens new avenues to explore the spatial determinants of female fertility and reproductive longevity.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Teaser<\/jats:title>\n                  <jats:p>Visium HD provides a comprehensive blueprint of the mouse ovary, revealing a medullary core of activation-poised pregranulosa cells.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.09.16.676655","type":"posted-content","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T22:40:11Z","timestamp":1758148811000},"source":"Crossref","is-referenced-by-count":3,"title":["Single-cell-scale spatial transcriptome reveals early regional priming of the developing mouse ovary"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6281-2632","authenticated-orcid":false,"given":"Anthony S.","family":"Martinez","sequence":"first","affiliation":[{"name":"Section of Developmental Biology, Department of Pediatrics, School of Medicine, University of Colorado Anschutz (Aurora, CO, USA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2796-2176","authenticated-orcid":false,"given":"Tyler J.","family":"Gibson","sequence":"additional","affiliation":[{"name":"Section of Developmental Biology, Department of Pediatrics, School of Medicine, University of Colorado Anschutz (Aurora, CO, USA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1141-3494","authenticated-orcid":false,"given":"Courtney","family":"Diamond","sequence":"additional","affiliation":[{"name":"Section of Developmental Biology, Department of Pediatrics, School of Medicine, University of Colorado Anschutz (Aurora, CO, USA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0870-2149","authenticated-orcid":false,"given":"Jennifer","family":"Jaime","sequence":"additional","affiliation":[{"name":"Section of Developmental Biology, Department of Pediatrics, School of Medicine, University of Colorado Anschutz (Aurora, CO, USA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2640-1502","authenticated-orcid":false,"given":"Jennifer","family":"McKey","sequence":"additional","affiliation":[{"name":"Section of Developmental Biology, Department of Pediatrics, School of Medicine, University of Colorado Anschutz (Aurora, CO, USA)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026030210350798000_2025.09.16.676655v2.1","doi-asserted-by":"publisher","DOI":"10.1159\/000358072"},{"key":"2026030210350798000_2025.09.16.676655v2.2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1159\/000526426","article-title":"Guided by Single-Cell Sequencing, of the Establishment and Maintenance of the Ovarian Reserve in Mammals","volume":"17","year":"2023","journal-title":"Sex Dev"},{"key":"2026030210350798000_2025.09.16.676655v2.3","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1056\/NEJMcp2116488","article-title":"Primary Ovarian Insufficiency","volume":"388","year":"2023","journal-title":"N Engl J Med"},{"key":"2026030210350798000_2025.09.16.676655v2.4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003629"},{"key":"2026030210350798000_2025.09.16.676655v2.5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1159\/000342072","article-title":"Precious cargo: regulation of sex-specific germ cell development in mice","volume":"7","year":"2013","journal-title":"Sex Dev"},{"key":"2026030210350798000_2025.09.16.676655v2.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2019.02.004"},{"key":"2026030210350798000_2025.09.16.676655v2.7","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9068"},{"key":"2026030210350798000_2025.09.16.676655v2.8","doi-asserted-by":"publisher","DOI":"10.1530\/JME-17-0314"},{"key":"2026030210350798000_2025.09.16.676655v2.9","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1986.66.1.71"},{"key":"2026030210350798000_2025.09.16.676655v2.10","doi-asserted-by":"crossref","unstructured":"K. Imaimatsu , A. Uchida , R. Hiramatsu , Y. Kanai , Gonadal Sex Differentiation and Ovarian Organogenesis along the Cortical-Medullary Axis in Mammals. Int J Mol Sci 23, 13373 (2022).","DOI":"10.3390\/ijms232113373"},{"key":"2026030210350798000_2025.09.16.676655v2.11","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.6.1603"},{"key":"2026030210350798000_2025.09.16.676655v2.12","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.17.3323"},{"key":"2026030210350798000_2025.09.16.676655v2.13","doi-asserted-by":"publisher","DOI":"10.1242\/dev.093864"},{"key":"2026030210350798000_2025.09.16.676655v2.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.05.001"},{"key":"2026030210350798000_2025.09.16.676655v2.15","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.1016\/j.celrep.2019.02.069","article-title":"Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics","volume":"26","year":"2019","journal-title":"Cell Reports"},{"key":"2026030210350798000_2025.09.16.676655v2.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00327-5"},{"key":"2026030210350798000_2025.09.16.676655v2.17","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.gde.2015.03.003","article-title":"Cell fate commitment during mammalian sex determination","volume":"32","year":"2015","journal-title":"Current Opinion in Genetics & Development"},{"key":"2026030210350798000_2025.09.16.676655v2.18","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.096651"},{"key":"2026030210350798000_2025.09.16.676655v2.19","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1471-213X-10-2","article-title":"Intact fetal ovarian cord formation promotes mouse oocyte survival and development","volume":"10","year":"2010","journal-title":"BMC Developmental Biology"},{"key":"2026030210350798000_2025.09.16.676655v2.20","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-14-0062"},{"key":"2026030210350798000_2025.09.16.676655v2.21","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2480-6"},{"key":"2026030210350798000_2025.09.16.676655v2.22","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0269"},{"key":"2026030210350798000_2025.09.16.676655v2.23","doi-asserted-by":"publisher","DOI":"10.1530\/REP-11-0299"},{"key":"2026030210350798000_2025.09.16.676655v2.24","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095208"},{"key":"2026030210350798000_2025.09.16.676655v2.25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2005570117"},{"key":"2026030210350798000_2025.09.16.676655v2.26","doi-asserted-by":"crossref","unstructured":"Q. Yin , A. C. Spradling , Distinct waves of ovarian follicles contribute to mouse oocyte production. eLife 14 (2025).","DOI":"10.7554\/eLife.107352.2"},{"key":"2026030210350798000_2025.09.16.676655v2.27","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3000"},{"key":"2026030210350798000_2025.09.16.676655v2.28","first-page":"291","article-title":"Ovarian Organogenesis in Mammals: Mice Cannot Tell Us Everything","volume":"3","year":"2010","journal-title":"Sex Dev"},{"key":"2026030210350798000_2025.09.16.676655v2.29","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81088"},{"key":"2026030210350798000_2025.09.16.676655v2.30","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod47.3.466"},{"key":"2026030210350798000_2025.09.16.676655v2.31","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt486"},{"key":"2026030210350798000_2025.09.16.676655v2.32","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.131227"},{"key":"2026030210350798000_2025.09.16.676655v2.33","doi-asserted-by":"publisher","DOI":"10.1101\/2024.08.27.609995"},{"key":"2026030210350798000_2025.09.16.676655v2.34","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm0972"},{"key":"2026030210350798000_2025.09.16.676655v2.35","doi-asserted-by":"publisher","DOI":"10.1101\/2025.09.24.678234"},{"key":"2026030210350798000_2025.09.16.676655v2.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.009"},{"key":"2026030210350798000_2025.09.16.676655v2.37","doi-asserted-by":"publisher","DOI":"10.1101\/2022.10.24.513438"},{"key":"2026030210350798000_2025.09.16.676655v2.38","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/978-1-4939-4017-2_10","article-title":"Single-Cell Lineage Analysis of Oogenesis in Mice","volume":"1463","year":"2017","journal-title":"Methods Mol Biol"},{"key":"2026030210350798000_2025.09.16.676655v2.39","doi-asserted-by":"crossref","first-page":"eaea7403","DOI":"10.1126\/sciadv.aea7403","article-title":"Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation","volume":"12","year":"2026","journal-title":"Sci Adv"},{"key":"2026030210350798000_2025.09.16.676655v2.40","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adl0788"},{"key":"2026030210350798000_2025.09.16.676655v2.41","first-page":"1","article-title":"Dissecting the fate of Foxl2-expressing cells in fetal ovary using lineage tracing and single-cell transcriptomics","volume":"8","year":"2022","journal-title":"Cell Discov"},{"key":"2026030210350798000_2025.09.16.676655v2.42","doi-asserted-by":"crossref","unstructured":"B. Soygur , D. J. Laird , Ovary Development: Insights From a Three-Dimensional Imaging Revolution. Frontiers in Cell and Developmental Biology 9 (2021).","DOI":"10.3389\/fcell.2021.698315"},{"key":"2026030210350798000_2025.09.16.676655v2.43","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc6747"},{"key":"2026030210350798000_2025.09.16.676655v2.44","doi-asserted-by":"crossref","first-page":"dev199208","DOI":"10.1242\/dev.199208","article-title":"Concerted morphogenesis of genital ridges and nephric ducts in the mouse captured through whole-embryo imaging","volume":"148","year":"2021","journal-title":"Development"},{"key":"2026030210350798000_2025.09.16.676655v2.45","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23872"},{"key":"2026030210350798000_2025.09.16.676655v2.46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.18.7215"},{"key":"2026030210350798000_2025.09.16.676655v2.47","doi-asserted-by":"crossref","unstructured":"C. Bunce , J. McKey , B. Capel , Concerted morphogenesis of genital ridges and nephric ducts in the mouse captured through whole-embryo imaging. Development 148 (2021).","DOI":"10.1242\/dev.199208"},{"key":"2026030210350798000_2025.09.16.676655v2.48","doi-asserted-by":"crossref","first-page":"e2401123","DOI":"10.1002\/smtd.202401123","article-title":"Sainsc: A Computational Tool for Segmentation-Free Analysis of In Situ Capture Data","volume":"9","year":"2025","journal-title":"Small Methods"},{"key":"2026030210350798000_2025.09.16.676655v2.49","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.96662.3"},{"key":"2026030210350798000_2025.09.16.676655v2.50","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026030210350798000_2025.09.16.676655v2.51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100920118"},{"key":"2026030210350798000_2025.09.16.676655v2.52","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.09.016"},{"key":"2026030210350798000_2025.09.16.676655v2.53","doi-asserted-by":"publisher","DOI":"10.1210\/en.140.12.5789"},{"key":"2026030210350798000_2025.09.16.676655v2.54","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240601"},{"key":"2026030210350798000_2025.09.16.676655v2.55","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0119"},{"key":"2026030210350798000_2025.09.16.676655v2.56","doi-asserted-by":"publisher","DOI":"10.1016\/b978-0-12-571148-7.50005-1"},{"key":"2026030210350798000_2025.09.16.676655v2.57","doi-asserted-by":"publisher","DOI":"10.1210\/endo-114-4-1315"},{"key":"2026030210350798000_2025.09.16.676655v2.58","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)60550-5"},{"key":"2026030210350798000_2025.09.16.676655v2.59","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-14-2-152"},{"key":"2026030210350798000_2025.09.16.676655v2.60","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1242\/dev.113.2.613","article-title":"Expression of the mouse anti-m\u00fcllerian hormone gene suggests a role in both male and female sexual differentiation","volume":"113","year":"1991","journal-title":"Development"},{"key":"2026030210350798000_2025.09.16.676655v2.61","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1038\/s41588-025-02193-3","article-title":"Visium HD Development Team, S. E. B. Taylor, High-definition spatial transcriptomic profiling of immune cell populations in colorectal cancer","volume":"57","year":"2025","journal-title":"Nat Genet"},{"key":"2026030210350798000_2025.09.16.676655v2.62","doi-asserted-by":"publisher","DOI":"10.1101\/2025.09.22.677812"},{"key":"2026030210350798000_2025.09.16.676655v2.63","doi-asserted-by":"crossref","first-page":"7539","DOI":"10.1038\/s41467-025-62702-0","article-title":"Defining the cell and molecular origins of the primate ovarian reserve","volume":"16","year":"2025","journal-title":"Nat Commun"},{"key":"2026030210350798000_2025.09.16.676655v2.64","doi-asserted-by":"crossref","unstructured":"M. Luo , X. Yang , M. Zhou , J. Zhang , B. Yu , H. Lian , J. Ye , Integrated single-cell and spatial transcriptomics reveal microenvironment disruptions by androgen in mouse ovary. iScience 27 (2024).","DOI":"10.1016\/j.isci.2024.111028"},{"key":"2026030210350798000_2025.09.16.676655v2.65","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2317418121"},{"key":"2026030210350798000_2025.09.16.676655v2.66","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adm7506"},{"key":"2026030210350798000_2025.09.16.676655v2.67","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1038\/s43587-024-00607-1","article-title":"Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1","volume":"4","year":"2024","journal-title":"Nat Aging"},{"key":"2026030210350798000_2025.09.16.676655v2.68","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/s42003-025-08277-4","article-title":"Spatiotemporal and single-cell atlases to dissect regional specific cell types of the developing ovary","volume":"8","year":"2025","journal-title":"Commun Biol"},{"key":"2026030210350798000_2025.09.16.676655v2.69","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1093\/jmcb\/mjz059","article-title":"SP1 governs primordial folliculogenesis by regulating pregranulosa cell development in mice","volume":"12","year":"2020","journal-title":"J Mol Cell Biol"},{"key":"2026030210350798000_2025.09.16.676655v2.70","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm0972"},{"key":"2026030210350798000_2025.09.16.676655v2.71","doi-asserted-by":"publisher","DOI":"10.1530\/REP-09-0392"},{"key":"2026030210350798000_2025.09.16.676655v2.72","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.04.006"},{"key":"2026030210350798000_2025.09.16.676655v2.73","doi-asserted-by":"publisher","DOI":"10.1071\/RD12134"},{"key":"2026030210350798000_2025.09.16.676655v2.74","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814930116"},{"key":"2026030210350798000_2025.09.16.676655v2.75","doi-asserted-by":"crossref","first-page":"eadx0659","DOI":"10.1126\/science.adx0659","article-title":"Comparative analysis of human and mouse ovaries across age","volume":"390","year":"2025","journal-title":"Science"},{"key":"2026030210350798000_2025.09.16.676655v2.76","first-page":"1732988","article-title":"Type VIII collagen: advances in matrix biology and translational promise","volume":"13","year":"2025","journal-title":"Front Bioeng Biotechnol"},{"key":"2026030210350798000_2025.09.16.676655v2.77","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0450201"},{"key":"2026030210350798000_2025.09.16.676655v2.78","doi-asserted-by":"crossref","first-page":"eadv1885","DOI":"10.1126\/sciadv.adv1885","article-title":"The gene regulatory landscape driving mouse gonadal supporting cell differentiation","volume":"11","year":"2025","journal-title":"Sci Adv"},{"key":"2026030210350798000_2025.09.16.676655v2.79","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gau007"},{"key":"2026030210350798000_2025.09.16.676655v2.80","doi-asserted-by":"publisher","DOI":"10.1097\/MED.0000000000000442"},{"key":"2026030210350798000_2025.09.16.676655v2.81","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-16-0522"},{"key":"2026030210350798000_2025.09.16.676655v2.82","doi-asserted-by":"crossref","first-page":"626924","DOI":"10.3389\/fendo.2021.626924","article-title":"Current Understanding of the Etiology, Symptomatology, and Treatment Options in Premature Ovarian Insufficiency (POI)","volume":"12","year":"2021","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2026030210350798000_2025.09.16.676655v2.83","doi-asserted-by":"crossref","unstructured":"S. Federici , R. Rossetti , S. Moleri , E. V. Munari , M. Frixou , M. Bonomi , L. Persani , Primary ovarian insufficiency: update on clinical and genetic findings. Front. Endocrinol. 15 (2024).","DOI":"10.3389\/fendo.2024.1464803"},{"key":"2026030210350798000_2025.09.16.676655v2.84","doi-asserted-by":"publisher","DOI":"10.1080\/13697137.2019.1574738"},{"key":"2026030210350798000_2025.09.16.676655v2.85","doi-asserted-by":"crossref","first-page":"giaf076","DOI":"10.1093\/gigascience\/giaf076","article-title":"SeuratExtend: streamlining single-cell RNA-seq analysis through an integrated and intuitive framework","volume":"14","year":"2025","journal-title":"Gigascience"},{"key":"2026030210350798000_2025.09.16.676655v2.86","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0113-3"},{"key":"2026030210350798000_2025.09.16.676655v2.87","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02212-x"},{"key":"2026030210350798000_2025.09.16.676655v2.88","doi-asserted-by":"publisher","DOI":"10.1109\/mcse.2007.55"},{"key":"2026030210350798000_2025.09.16.676655v2.89","unstructured":"H. Wickham , Ggplot2 (Springer International Publishing, Cham, 2016; http:\/\/link.springer.com\/10.1007\/978-3-319-24277-4)Use R!"},{"key":"2026030210350798000_2025.09.16.676655v2.90","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac057"},{"key":"2026030210350798000_2025.09.16.676655v2.91","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-1950-6"},{"key":"2026030210350798000_2025.09.16.676655v2.92","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.73600.2"},{"key":"2026030210350798000_2025.09.16.676655v2.93","doi-asserted-by":"crossref","unstructured":"L. Folts , A. S. Martinez , J. A. Williams , C. Bunce , B. Capel , J. McKey , OoCount: A Machine-Learning Based Approach to Mouse Ovarian Follicle Counting and Classification. Biology of Reproduction, ioaf023 (2025).","DOI":"10.1101\/2024.05.13.593993"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.09.16.676655","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T18:35:17Z","timestamp":1772476517000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.09.16.676655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,17]]},"references-count":93,"URL":"https:\/\/doi.org\/10.1101\/2025.09.16.676655","relation":{},"subject":[],"published":{"date-parts":[[2025,9,17]]},"subtype":"preprint"}}