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PKD patients exhibit significant heterogeneity in disease progression, largely due to genetic and environmental modifiers. Like patients, mouse models of PKD also exhibit significant heterogeneity with regards to the gene mutated, age of disease onset, and rate of disease progression. To elucidate the cellular and molecular consequences of these variables, we constructed an integrated single-cell and spatial transcriptomics atlas across mouse models of PKD, mapping changes in cell type composition, gene expression, and intercellular signaling networks across the whole atlas and within individual models. Consistently across models, single cell RNA sequencing (scRNAseq) data revealed increased\n                  <jats:italic>Spp1<\/jats:italic>\n                  (osteopontin) expression and signaling from PKD-enriched clusters to Ly6c\n                  <jats:sup>lo<\/jats:sup>\n                  monocytes. Global deletion of\n                  <jats:italic>Spp1<\/jats:italic>\n                  in\n                  <jats:italic>Pkd1<\/jats:italic>\n                  <jats:sup>RC\/RC<\/jats:sup>\n                  mice resulted in reduced cyst severity, improved kidney function, and reduced Ly6c\n                  <jats:sup>lo<\/jats:sup>\n                  monocyte numbers, suggesting that SPP1 signaling to Ly6c\n                  <jats:sup>lo<\/jats:sup>\n                  monocytes promotes PKD progression. We also created a freely available, searchable website (\n                  <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/bmblx.bmi.osumc.edu\/scPKD\/\">https:\/\/bmblx.bmi.osumc.edu\/scPKD\/<\/jats:ext-link>\n                  ) that can be used to identify cross- and intra-model specific changes in gene expression, guiding researchers to new therapeutic targets for treating PKD.\n                <\/jats:p>","DOI":"10.1101\/2025.09.17.676846","type":"posted-content","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T16:50:17Z","timestamp":1758387017000},"source":"Crossref","is-referenced-by-count":0,"title":["A cross model spatial and single-cell atlas reveals the conserved involvement of osteopontin in polycystic kidney disease"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4888-5306","authenticated-orcid":false,"given":"Sarah 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