{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T20:27:00Z","timestamp":1788208020391,"version":"build-2803163510"},"posted":{"date-parts":[[2025,9,29]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":29,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,29]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  RH5-Interacting Protein (RIPR) is essential for the invasion of\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  into host red blood cells and is currently being studied as a novel malaria vaccine candidate in Phase 1a clinical trials. To study the genetic diversity of RIPR, deep amplicon sequencing was used to identify RIPR mutations in\n                  <jats:italic>Plasmodium falciparum<\/jats:italic>\n                  clinical isolates (n=89) collected in K\u00e9dougou, a high malaria transmission region of Senegal. We identified nonsynonymous single nucleotide polymorphisms (SNPs) in 64\/89 (71.9%) of the samples. In total, 26 non-synonymous SNPs were identified, of which 15 were novel. 16\/26 SNPs were able to be threaded onto existing RIPR crystal structures to predict the effects of SNPs on RIPR stability. 7\/16 mutations were predicted to destabilize RIPR while 2\/16 increased the stability of RIPR. Additionally, we identified 3 SNPs (Q737K, T738K, V840L) in the EGF5-8 domains of RIPR where neutralizing antibodies are known to bind.\n                <\/jats:p>","DOI":"10.1101\/2025.09.23.25335263","type":"posted-content","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T22:55:19Z","timestamp":1759186519000},"source":"Crossref","is-referenced-by-count":1,"title":["Genetic Diversity of the Malaria Vaccine Candidate PfRIPR in a High Transmission Region of 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