{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:18:05Z","timestamp":1785417485777,"version":"3.56.0"},"posted":{"date-parts":[[2025,9,25]]},"group-title":"Neuroscience","reference-count":101,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["U19NS130608"],"award-info":[{"award-number":["U19NS130608"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]},{"award":["NS078226"],"award-info":[{"award-number":["NS078226"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Guillain-Barr\u00e9 syndrome (GBS) is an autoimmune disorder that causes weakness, sensory loss, autonomic dysfunction, and chronic neuropathic pain. The mediators responsible for driving early autoimmune injury in the most common GBS variant, acute inflammatory demyelinating polyradiculoneuropathy (AIDP), remain incompletely understood. We performed single-cell and bulk RNA sequencing on peripheral blood mononuclear cells collected from early untreated AIDP-variant GBS patients and healthy controls to comprehensively deduce leukocyte transcriptome alterations and predict disease- and pain-driving interactions between pathogenic leukocytes and peripheral nervous system cells. We found that classical, intermediate, and non-classical monocytes were expanded and upregulated genes associated with type I and II interferons, JAK\/STAT signaling, and NLRP3 inflammasome engagement. CD8+ T cells were highly proliferative and likewise upregulated JAK\/STAT signaling.\n                  <jats:italic>CD4+FOXP3+<\/jats:italic>\n                  regulatory T cells upregulated\n                  <jats:italic>PRDM1<\/jats:italic>\n                  and\n                  <jats:italic>CD74<\/jats:italic>\n                  in a signature that may indicate functional exhaustion. A subpopulation of highly activated intermediate monocytes upregulated genes related to angiogenesis and oncostatin M. Differential expression-based cell-cell interaction analysis between GBS leukocytes, Schwann cells, and sensory neurons predicted engagement of ligand-receptor pairs with nerve integrity and pain functions, including epiregulin, interferon-beta, adrenomedullin, clusterin, IL-6, IL-15, and CCL4. Functional validation demonstrated that CCL4 sensitizes human sensory neurons\n                  <jats:italic>in vitro<\/jats:italic>\n                  . These results unearth molecular interactions by which specific leukocyte populations in AIDP-variant GBS may participate in peripheral nerve injury and drive neuropathic pain. Many of these targets may be amenable to therapeutic modulation using available approved and investigational drugs, potentially providing drug repurposing opportunities.\n                <\/jats:p>","DOI":"10.1101\/2025.09.24.678413","type":"posted-content","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T03:30:21Z","timestamp":1758857421000},"source":"Crossref","is-referenced-by-count":2,"title":["Nerve-targeting and pain-promoting transcriptomic signatures in early Guillain-Barr\u00e9 syndrome"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5305-4350","authenticated-orcid":false,"given":"Jayden A.","family":"O\u2019Brien","sequence":"first","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph B.","family":"Lesnak","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishwarya","family":"Sankaranarayanan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asta","family":"Arendt-Tranholm","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Inturi","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spoorthi","family":"Sadasivuni","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemanth","family":"Mydugolam","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2078-3527","authenticated-orcid":false,"given":"Katelyn E.","family":"Sadler","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6971-6221","authenticated-orcid":false,"given":"Theodore J.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eroboghene E.","family":"Ubogu","sequence":"additional","affiliation":[{"name":"Division of Neuromuscular Disease, Department of Neurology, University of Alabama at Birmingham."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"8","key":"2026062709101428000_2025.09.24.678413v2.1","doi-asserted-by":"crossref","first-page":"e16365","DOI":"10.1111\/ene.16365","article-title":"Guillain-Barre syndrome: a comprehensive review","volume":"31","year":"2024","journal-title":"Eur J Neurol"},{"issue":"1","key":"2026062709101428000_2025.09.24.678413v2.2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1186\/s12974-021-02319-4","article-title":"Global, regional, and national burden of Guillain-Barre syndrome and its underlying causes from 1990 to 2019","volume":"18","year":"2021","journal-title":"J Neuroinflammation"},{"issue":"11","key":"2026062709101428000_2025.09.24.678413v2.3","first-page":"2568","article-title":"Immunotherapy of Guillain-Barre syndrome","volume":"14","year":"2018","journal-title":"Hum Vaccin Immunother"},{"key":"2026062709101428000_2025.09.24.678413v2.4","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2014.121"},{"key":"2026062709101428000_2025.09.24.678413v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00339-1"},{"key":"2026062709101428000_2025.09.24.678413v2.6","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aww247"},{"key":"2026062709101428000_2025.09.24.678413v2.7","first-page":"740947","article-title":"Circulating Th17, Th22, and Th1 cells are elevated in the Guillain-Barre syndrome and downregulated by IVIg treatments","volume":"2014","year":"2014","journal-title":"Mediators Inflamm"},{"key":"2026062709101428000_2025.09.24.678413v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2013.05.005"},{"key":"2026062709101428000_2025.09.24.678413v2.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06916-6"},{"key":"2026062709101428000_2025.09.24.678413v2.10","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880150506"},{"key":"2026062709101428000_2025.09.24.678413v2.11","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/ana.410090713","article-title":"Immunological characterization of sural nerve biopsies from patients with Guillain-Barre syndrome","volume":"9","year":"1981","journal-title":"Ann Neurol"},{"key":"2026062709101428000_2025.09.24.678413v2.12","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(99)00328-7"},{"key":"2026062709101428000_2025.09.24.678413v2.13","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199604)19:4<474::AID-MUS8>3.0.CO;2-9"},{"key":"2026062709101428000_2025.09.24.678413v2.14","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1599-0"},{"key":"2026062709101428000_2025.09.24.678413v2.15","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1466-4"},{"issue":"1","key":"2026062709101428000_2025.09.24.678413v2.16","first-page":"19","article-title":"Guillain-Barre syndrome: an ultrastructural study of peripheral nerve in 65 patients","volume":"6","year":"1987","journal-title":"Clin Neuropathol"},{"issue":"2","key":"2026062709101428000_2025.09.24.678413v2.17","first-page":"133","article-title":"An electron microscope study","volume":"26","author":"Acute idiopathic polyneuritis","year":"1972","journal-title":"Lab Invest"},{"key":"2026062709101428000_2025.09.24.678413v2.18","doi-asserted-by":"crossref","unstructured":"Prineas JW . Pathology of the Guillain-Barre syndrome. Ann Neurol. 1981;9 Suppl:6-19.","DOI":"10.1002\/ana.410090704"},{"key":"2026062709101428000_2025.09.24.678413v2.19","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20076"},{"issue":"4","key":"2026062709101428000_2025.09.24.678413v2.20","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.jocn.2009.07.096","article-title":"T cells from patients with Guillain-Barre syndrome produce interferon-gamma in response to stimulation with the ganglioside GM1","volume":"17","year":"2010","journal-title":"J Clin Neurosci"},{"key":"2026062709101428000_2025.09.24.678413v2.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2007.09.034"},{"key":"2026062709101428000_2025.09.24.678413v2.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocn.2007.09.016"},{"key":"2026062709101428000_2025.09.24.678413v2.23","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz063"},{"key":"2026062709101428000_2025.09.24.678413v2.24","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abe1648"},{"key":"2026062709101428000_2025.09.24.678413v2.25","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abj8186"},{"key":"2026062709101428000_2025.09.24.678413v2.26","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adj9173)"},{"key":"2026062709101428000_2025.09.24.678413v2.27","doi-asserted-by":"crossref","unstructured":"Ray PR , Shiers S , Caruso JP , Tavares-Ferreira D , Sankaranarayanan I , Uhelski ML , et al. RNA profiling of human dorsal root ganglia reveals sex-differences in mechanisms promoting neuropathic pain. Brain: A Journal of Neurology. 2022:awac266.","DOI":"10.1093\/brain\/awac266"},{"key":"2026062709101428000_2025.09.24.678413v2.28","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00561-x"},{"key":"2026062709101428000_2025.09.24.678413v2.29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb03325.x"},{"key":"2026062709101428000_2025.09.24.678413v2.30","doi-asserted-by":"publisher","DOI":"10.1038\/ni1073"},{"key":"2026062709101428000_2025.09.24.678413v2.31","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2019.01.070"},{"key":"2026062709101428000_2025.09.24.678413v2.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2018.04.003"},{"key":"2026062709101428000_2025.09.24.678413v2.33","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2006"},{"issue":"5","key":"2026062709101428000_2025.09.24.678413v2.34","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1016\/j.immuni.2026.03.005","article-title":"Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation","volume":"59","year":"2026","journal-title":"Immunity"},{"issue":"28","key":"2026062709101428000_2025.09.24.678413v2.35","first-page":"e2206692","article-title":"BATF is Required for Treg Homeostasis and Stability to Prevent Autoimmune Pathology","volume":"10","year":"2023","journal-title":"Adv Sci (Weinh"},{"key":"2026062709101428000_2025.09.24.678413v2.36","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abk0957"},{"key":"2026062709101428000_2025.09.24.678413v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.01.022"},{"issue":"6","key":"2026062709101428000_2025.09.24.678413v2.38","doi-asserted-by":"crossref","first-page":"111606","DOI":"10.1016\/j.celrep.2022.111606","article-title":"Single-cell transcriptome profiling and chromatin accessibility reveal an exhausted regulatory CD4+ T cell subset in systemic lupus erythematosus","volume":"41","year":"2022","journal-title":"Cell Reports"},{"key":"2026062709101428000_2025.09.24.678413v2.39","doi-asserted-by":"crossref","unstructured":"Franco-Enz\u00e1stiga \u00da , Natarajan K , David ET , Patel K , Ravirala A , and Price TJ . Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction. iScience. 2024;0(0).","DOI":"10.1101\/2023.06.03.543579"},{"key":"2026062709101428000_2025.09.24.678413v2.40","doi-asserted-by":"crossref","unstructured":"Franco-Enzastiga U , Natarajan K , Espinosa F , Granja-Vazquez R , Mydugolam H , and Price TJ . Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization. JCI Insight. 2025.","DOI":"10.1101\/2025.04.22.650068"},{"key":"2026062709101428000_2025.09.24.678413v2.41","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3822-13.2014"},{"key":"2026062709101428000_2025.09.24.678413v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04183-x"},{"key":"2026062709101428000_2025.09.24.678413v2.43","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-32427-5"},{"key":"2026062709101428000_2025.09.24.678413v2.44","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2006.12.002"},{"issue":"10","key":"2026062709101428000_2025.09.24.678413v2.45","first-page":"19126","article-title":"Expression and significance of CD4(+)CD25(+)CD127(-) regulatory T cells in peripheral blood of patients with different phenotypes of Guillain-Barre syndrome","volume":"8","year":"2015","journal-title":"Int J Clin Exp Med"},{"issue":"2-3","key":"2026062709101428000_2025.09.24.678413v2.46","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1007\/s12026-014-8580-6","article-title":"Intravenous immunoglobulin exerts reciprocal regulation of Th1\/Th17 cells and regulatory T cells in Guillain-Barre syndrome patients","volume":"60","year":"2014","journal-title":"Immunol Res"},{"key":"2026062709101428000_2025.09.24.678413v2.47","doi-asserted-by":"crossref","unstructured":"Zhang G , Wang Q , Song Y , Cheng P , Xu R , Feng X , et al. Intravenous immunoglobulin promotes the proliferation of CD4(+)CD25(+) Foxp3(+) regulatory T cells and the cytokines secretion in patients with Guillain-Barre syndrome in vitro. J Neuroimmunol. 2019;336:577042.","DOI":"10.1016\/j.jneuroim.2019.577042"},{"issue":"3","key":"2026062709101428000_2025.09.24.678413v2.48","doi-asserted-by":"crossref","first-page":"e70039","DOI":"10.1111\/jns.70039","article-title":"Intravenous Immunoglobulin Elevates Regulatory T Cells in Guillain-Barre Syndrome: A Potential Biomarker of Therapeutic Response","volume":"30","year":"2025","journal-title":"J Peripher Nerv Syst"},{"key":"2026062709101428000_2025.09.24.678413v2.49","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-016-0995-1"},{"key":"2026062709101428000_2025.09.24.678413v2.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2008.01.005"},{"key":"2026062709101428000_2025.09.24.678413v2.51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1710566114"},{"key":"2026062709101428000_2025.09.24.678413v2.52","doi-asserted-by":"crossref","unstructured":"Bedoui Y , De Larichaudy D , Daniel M , Ah-Pine F , Selambarom J , Guiraud P , et al. Deciphering the Role of Schwann Cells in Inflammatory Peripheral Neuropathies Post Alphavirus Infection. Cells. 2022;12(1).","DOI":"10.3390\/cells12010100"},{"key":"2026062709101428000_2025.09.24.678413v2.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2016.06.001"},{"key":"2026062709101428000_2025.09.24.678413v2.54","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adh9603"},{"key":"2026062709101428000_2025.09.24.678413v2.55","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-03151-1"},{"key":"2026062709101428000_2025.09.24.678413v2.56","doi-asserted-by":"crossref","unstructured":"Tan PH , Ji J , Yeh CC , and Ji RR . Interferons in Pain and Infections: Emerging Roles in Neuro-Immune and Neuro-Glial Interactions. Front Immunol. 2021;12:783725.","DOI":"10.3389\/fimmu.2021.783725"},{"key":"2026062709101428000_2025.09.24.678413v2.57","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3055-19.2020"},{"key":"2026062709101428000_2025.09.24.678413v2.58","unstructured":"Bhuiyan SA , Nagi SS , Sankaranarayanan I , Semizoglou E , Usoskin D , Yang L , et al. A Reference Atlas of the Human Dorsal Root Ganglion. bioRxiv. 2025:2025.11.05.686654."},{"key":"2026062709101428000_2025.09.24.678413v2.59","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abq6691"},{"key":"2026062709101428000_2025.09.24.678413v2.60","doi-asserted-by":"publisher","DOI":"10.1172\/JCI87406"},{"issue":"5","key":"2026062709101428000_2025.09.24.678413v2.61","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s40262-025-01506-3","article-title":"Population Pharmacokinetics and Pharmacodynamics of Fepixnebart (LY3016859) and Epiregulin in Patients with Chronic Pain","volume":"64","year":"2025","journal-title":"Clin Pharmacokinet"},{"key":"2026062709101428000_2025.09.24.678413v2.62","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.113.210765"},{"key":"2026062709101428000_2025.09.24.678413v2.63","first-page":"1744-8069","article-title":"Oncostatin M Induces Heat Hypersensitivity by gp130-Dependent Sensitization of TRPV1 in Sensory Neurons","volume":"7","year":"2011","journal-title":"Molecular Pain"},{"key":"2026062709101428000_2025.09.24.678413v2.64","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4975-03.2004"},{"key":"2026062709101428000_2025.09.24.678413v2.65","doi-asserted-by":"crossref","unstructured":"Mwirigi JM , Sankaranarayanan I , Tavares-Ferreira D , Gabriel KA , Palomino S , Li Y , et al. Expansion of OSMR expression and signaling in the human dorsal root ganglion links OSM to neuropathic pain. bioRxiv. 2025:2025.03.26.645611.","DOI":"10.1101\/2025.03.26.645611"},{"key":"2026062709101428000_2025.09.24.678413v2.66","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keac300"},{"issue":"2","key":"2026062709101428000_2025.09.24.678413v2.67","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1001\/jamadermatol.2025.4950","article-title":"Vixarelimab in Patients With Prurigo Nodularis: A Randomized Clinical Trial","volume":"162","year":"2026","journal-title":"JAMA Dermatol"},{"key":"2026062709101428000_2025.09.24.678413v2.68","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c03233"},{"key":"2026062709101428000_2025.09.24.678413v2.69","doi-asserted-by":"crossref","unstructured":"Ciechanowska A , Pawlik K , Ciapala K , and Mika J . Pharmacological Modulation of the MIP-1 Family and Their Receptors Reduces Neuropathic Pain Symptoms and Influences Morphine Analgesia: Evidence from a Mouse Model. Brain Sci. 2023;13(4).","DOI":"10.3390\/brainsci13040579"},{"key":"2026062709101428000_2025.09.24.678413v2.70","doi-asserted-by":"publisher","DOI":"10.1002\/j.1532-2149.2012.00146.x"},{"key":"2026062709101428000_2025.09.24.678413v2.71","doi-asserted-by":"crossref","unstructured":"O\u2019Brien JA , Kuttanna N , Mazhar K , Mancilla Moreno M , Arendt-Tranholm A , Lesnak JB , et al. T-cell distribution in the dorsal root ganglion across species, sex, and age. bioRxiv. 2026;2026.04.30.722027.","DOI":"10.64898\/2026.04.30.722027"},{"key":"2026062709101428000_2025.09.24.678413v2.72","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000244420.68037.86"},{"key":"2026062709101428000_2025.09.24.678413v2.73","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2734"},{"issue":"5","key":"2026062709101428000_2025.09.24.678413v2.74","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1097\/j.pain.0000000000003904","article-title":"A double-blind, placebo-controlled, multi-crossover trial of treatment with a chemokine antagonist for knee osteoarthritis pain","volume":"167","year":"2026","journal-title":"Pain"},{"key":"2026062709101428000_2025.09.24.678413v2.75","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410270707"},{"key":"2026062709101428000_2025.09.24.678413v2.76","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440512"},{"key":"2026062709101428000_2025.09.24.678413v2.77","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh655"},{"key":"2026062709101428000_2025.09.24.678413v2.78","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2006.06.004"},{"issue":"12","key":"2026062709101428000_2025.09.24.678413v2.79","doi-asserted-by":"crossref","first-page":"3857","DOI":"10.1002\/jcp.24100","article-title":"and Ubogu EE. alpha(M)beta(2)-integrin-intercellular adhesion molecule-1 interactions drive the flow-dependent trafficking of Guillain-Barre syndrome patient derived mononuclear leukocytes at the blood-nerve barrier in vitro","volume":"227","year":"2012","journal-title":"J Cell Physiol"},{"key":"2026062709101428000_2025.09.24.678413v2.80","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2017.02.012"},{"key":"2026062709101428000_2025.09.24.678413v2.81","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1114s51"},{"key":"2026062709101428000_2025.09.24.678413v2.82","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv098"},{"key":"2026062709101428000_2025.09.24.678413v2.83","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1112s47"},{"key":"2026062709101428000_2025.09.24.678413v2.84","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.095"},{"key":"2026062709101428000_2025.09.24.678413v2.85","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45839-z"},{"key":"2026062709101428000_2025.09.24.678413v2.86","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026062709101428000_2025.09.24.678413v2.87","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKZ114"},{"key":"2026062709101428000_2025.09.24.678413v2.88","doi-asserted-by":"publisher","DOI":"10.1038\/S41587-023-01767-Y"},{"key":"2026062709101428000_2025.09.24.678413v2.89","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-20-3562"},{"key":"2026062709101428000_2025.09.24.678413v2.90","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty895"},{"key":"2026062709101428000_2025.09.24.678413v2.91","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw377"},{"key":"2026062709101428000_2025.09.24.678413v2.92","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6202607"},{"key":"2026062709101428000_2025.09.24.678413v2.93","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti565"},{"key":"2026062709101428000_2025.09.24.678413v2.94","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1790-4"},{"key":"2026062709101428000_2025.09.24.678413v2.95","doi-asserted-by":"crossref","unstructured":"Lee JS , Park S , Jeong HW , Ahn JY , Choi SJ , Lee H , et al. Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19. Sci Immunol. 2020;5(49).","DOI":"10.1126\/sciimmunol.abd1554"},{"key":"2026062709101428000_2025.09.24.678413v2.96","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085422"},{"key":"2026062709101428000_2025.09.24.678413v2.97","doi-asserted-by":"crossref","unstructured":"Shiers S , Yousuf MS , Mwirigi J , Cervantes A , and Price T. Human Ganglia and Spinal Cord Tissue Procurement from Organ Donors and Tissue Quality Assessment. protocolsio. 2024.","DOI":"10.17504\/protocols.io.kqdg32qr1v25\/v1"},{"issue":"10","key":"2026062709101428000_2025.09.24.678413v2.98","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1038\/nprot.2016.111","article-title":"Surgical extraction of human dorsal root ganglia from organ donors and preparation of primary sensory neuron cultures","volume":"11","year":"2016","journal-title":"Nature Protocols"},{"key":"2026062709101428000_2025.09.24.678413v2.99","doi-asserted-by":"crossref","unstructured":"Lesnak JB , Schaub MK , Gomez K , Calderon-Rivera A , Loya-Lopez S , Stewart R , et al. Enabling wider access to human molecular neuroscience research in pain: A simple preservation method for human dorsal root ganglion neurons in Hibernate A media. bioRxiv. 2025:2025.09.06.674648.","DOI":"10.2139\/ssrn.5499728"},{"key":"2026062709101428000_2025.09.24.678413v2.100","unstructured":"Yousuf MS , Mwirigi JM , Mancilla Moreno M , Schackmuth MK , and Price TJ. Dissociating and culturing human dorsal root ganglia neurons. protocolsio. 2025."},{"key":"2026062709101428000_2025.09.24.678413v2.101","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.09.24.678413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:10:36Z","timestamp":1782576636000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.09.24.678413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,25]]},"references-count":101,"URL":"https:\/\/doi.org\/10.1101\/2025.09.24.678413","relation":{},"subject":[],"published":{"date-parts":[[2025,9,25]]},"subtype":"preprint"}}