{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:18:13Z","timestamp":1785403093280,"version":"3.56.0"},"posted":{"date-parts":[[2025,9,29]]},"group-title":"Bioengineering","reference-count":72,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R35GM128648"],"award-info":[{"award-number":["R35GM128648"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"award":["5TL1TR002318-08"],"award-info":[{"award-number":["5TL1TR002318-08"]}],"id":[{"id":"https:\/\/ror.org\/04pw6fb54","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UL1TR002319"],"award-info":[{"award-number":["UL1TR002319"]}],"id":[{"id":"https:\/\/ror.org\/04pw6fb54","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/00cvxb145","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Schmidt Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Remote blood sampling offers multiple advantages over traditional clinic-based blood sampling studies, including greater patient inclusion, more frequent sampling, and broader geographical reach. Combining remote blood sampling with transcriptomic analysis opens potential in translational applications for capturing acute and dynamic immune responses to various exposures. In this study, we establish the feasibility of homeRNA, a capillary blood collection and RNA\n                  <jats:italic>later<\/jats:italic>\n                  -based stabilization kit, for use in downstream bulk RNA-sequencing applications via capturing a lipopolysaccharide (LPS)-induced inflammatory response. We also compared the baseline gene expression profiles and induced inflammatory response following LPS stimulation between homeRNA-stabilized samples and venous blood stabilized with RNA\n                  <jats:italic>later<\/jats:italic>\n                  or PAXgene. We found that homeRNA was successfully able to capture an inflammatory response to LPS, specifically targeting various cytokines (e.g.,\n                  <jats:italic>IL6, IL12B, IL1B<\/jats:italic>\n                  ), chemokines (e.g.,\n                  <jats:italic>CCL3, CXCL10, CCL4<\/jats:italic>\n                  ), and other transcriptional factors in the toll-like receptor pathway, the primary pathway activated during LPS stimulation. Importantly, we also found that homeRNA captured a LPS-induced inflammatory response comparable to that of venous blood samples stabilized with either RNA\n                  <jats:italic>later<\/jats:italic>\n                  or PAXgene. Overall, this work demonstrates that the homeRNA platform is compatible with downstream bulk RNA-sequencing analysis and can capture transcriptomic immune responses to a known stimulus which are analogous to results in traditional stabilized venous blood samples.\n                <\/jats:p>","DOI":"10.1101\/2025.09.27.678977","type":"posted-content","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T23:20:20Z","timestamp":1759188020000},"source":"Crossref","is-referenced-by-count":0,"title":["From Home to Transcriptome: Comparing the transcriptomic profile of induced immune response via lipopolysaccharide stimulation in homeRNA and venous blood"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5932-2691","authenticated-orcid":false,"given":"Lauren G.","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofu","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8643-5686","authenticated-orcid":false,"given":"Laura A.","family":"Milton","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"},{"name":"School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, Australia"},{"name":"Centre for Biomedical Technologies, Queensland University of Technology, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Yunos","family":"Alizai","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo K.","family":"Bammler","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3751-2013","authenticated-orcid":false,"given":"Yuting","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4217-4777","authenticated-orcid":false,"given":"Ingrid H.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen N.","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4105-4852","authenticated-orcid":false,"given":"Yi-Chin","family":"Toh","sequence":"additional","affiliation":[{"name":"School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, Australia"},{"name":"Centre for Biomedical Technologies, Queensland University of Technology, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Chaussabel","sequence":"additional","affiliation":[{"name":"Computer Sciences Department, The Jackson Laboratory, Farmington, CT, 06032, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Berthier","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0066-7556","authenticated-orcid":false,"given":"Amanda J.","family":"Haack","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington 98195, United States"},{"name":"School of Medicine, University of Washington, Seattle, Washington 98195, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashleigh 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