{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T10:04:57Z","timestamp":1789293897149,"version":"build-2803163510"},"posted":{"date-parts":[[2025,10,1]]},"group-title":"Neuroscience","reference-count":29,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["1F32AA031395"],"award-info":[{"award-number":["1F32AA031395"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Adolescent binge drinking can lead to a myriad of issues in adulthood, including psychiatric comorbidities that worsen clinical prognosis. Efforts to understand the effects of adolescent alcohol exposure on later health outcomes have unveiled lasting effects on physiology of brain regions critical for associative learning processes, including the hippocampus and prefrontal cortex (PFC). Here, we tested the hypothesis that adolescent alcohol exposure would produce lasting alterations in physiology within the retrosplenial cortex (RSC), another region critical for associative learning that is reciprocally connected to the hippocampus and PFC. In support of this hypothesis, we found that adolescent intermittent ethanol (AIE) vapor exposure resulted in lasting reductions in intrinsic excitability in RSC pyramidal cells of adult mice. Importantly, these changes were sex-specific, occurring in males but not females. This work suggests that the RSC may be a key, vulnerable locus to adolescent alcohol\u2019s detrimental effects.<\/jats:p>\n                <jats:sec>\n                  <jats:title>NEW &amp; NOTEWORTHY<\/jats:title>\n                  <jats:p>This study is the first to demonstrate that adolescent alcohol exposure produces lasting alterations in adult retrosplenial cortex physiology, providing novel insight into how adolescent alcohol exerts detrimental effects on adult brain function.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.09.29.679287","type":"posted-content","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T18:30:23Z","timestamp":1759343423000},"source":"Crossref","is-referenced-by-count":0,"title":["Adolescent alcohol exposure disrupts retrosplenial cortex physiology in adult males"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9722-3811","authenticated-orcid":false,"given":"Lisa R","family":"Taxier","sequence":"first","affiliation":[{"name":"Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili S","family":"Kooyman","sequence":"additional","affiliation":[{"name":"Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sloan Y","family":"Markowitz","sequence":"additional","affiliation":[{"name":"Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0530-5482","authenticated-orcid":false,"given":"Jessica A","family":"Wojick","sequence":"additional","affiliation":[{"name":"Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4747-4495","authenticated-orcid":false,"given":"Thomas L","family":"Kash","sequence":"additional","affiliation":[{"name":"Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091302400664000_2025.09.29.679287v2.1","doi-asserted-by":"publisher","DOI":"10.1097\/01.alc.0000179368.87886.76"},{"key":"2026091302400664000_2025.09.29.679287v2.2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0550-18.2018"},{"key":"2026091302400664000_2025.09.29.679287v2.3","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.896880"},{"key":"2026091302400664000_2025.09.29.679287v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2023.109561"},{"key":"2026091302400664000_2025.09.29.679287v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/bs.irn.2021.08.005"},{"key":"2026091302400664000_2025.09.29.679287v2.6","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.157.5.737"},{"key":"2026091302400664000_2025.09.29.679287v2.7","doi-asserted-by":"publisher","DOI":"10.1124\/pr.115.012138"},{"key":"2026091302400664000_2025.09.29.679287v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2006.04.021"},{"key":"2026091302400664000_2025.09.29.679287v2.9","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.837657"},{"key":"2026091302400664000_2025.09.29.679287v2.10","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12725"},{"key":"2026091302400664000_2025.09.29.679287v2.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-12956-6"},{"key":"2026091302400664000_2025.09.29.679287v2.12","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0550-18.2018"},{"key":"2026091302400664000_2025.09.29.679287v2.13","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2733"},{"key":"2026091302400664000_2025.09.29.679287v2.14","doi-asserted-by":"publisher","DOI":"10.1177\/0284185120923270"},{"key":"2026091302400664000_2025.09.29.679287v2.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2022.02.013"},{"key":"2026091302400664000_2025.09.29.679287v2.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpsc.2018.11.012"},{"key":"2026091302400664000_2025.09.29.679287v2.17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2008.00693.x"},{"key":"2026091302400664000_2025.09.29.679287v2.18","doi-asserted-by":"publisher","DOI":"10.1001\/archpsyc.60.7.727"},{"key":"2026091302400664000_2025.09.29.679287v2.19","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12364"},{"key":"2026091302400664000_2025.09.29.679287v2.20","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00577.2019"},{"key":"2026091302400664000_2025.09.29.679287v2.21","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0525-24.2024"},{"key":"2026091302400664000_2025.09.29.679287v2.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2020.107266"},{"key":"2026091302400664000_2025.09.29.679287v2.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.12.093"},{"key":"2026091302400664000_2025.09.29.679287v2.24","doi-asserted-by":"publisher","DOI":"10.1111\/1467-8721.00072"},{"key":"2026091302400664000_2025.09.29.679287v2.25","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-020-0670-7"},{"key":"2026091302400664000_2025.09.29.679287v2.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2020.108309"},{"key":"2026091302400664000_2025.09.29.679287v2.27","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12725"},{"key":"2026091302400664000_2025.09.29.679287v2.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2014.04.012"},{"key":"2026091302400664000_2025.09.29.679287v2.29","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-024-01647-x"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.09.29.679287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T09:40:12Z","timestamp":1789292412000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.09.29.679287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,1]]},"references-count":29,"URL":"https:\/\/doi.org\/10.1101\/2025.09.29.679287","relation":{},"subject":[],"published":{"date-parts":[[2025,10,1]]},"subtype":"preprint"}}