{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T13:40:58Z","timestamp":1768570858724,"version":"3.49.0"},"posted":{"date-parts":[[2025,10,2]]},"group-title":"HIV\/AIDS","reference-count":15,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.medrxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>People living with HIV (PLWH) are at increased risk of cerebrovascular abnormalities, including aneurysmal subarachnoid hemorrhage (SAH). However, it is unclear whether HIV-associated inflammation contributes significantly to the inflammatory response observed in the cerebrospinal fluid (CSF) during aneurysm rupture. Here, we used high-throughput Olink proteomics to compare inflammatory marker profiles in CSF between participants with aneurysmal SAH and PLWH without aneurysms.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>This was a cross-sectional observational study which enrolled participants who were indicated for endovascular coil embolisation due to ruptured anterior communicating artery aneurysms (n=30) or undergoing clinically indicated lumbar puncture as part of workup for a non-neurovascular condition (n=9).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We performed lumbar puncture and analyzed CSF samples from individuals presenting with aneurysmal SAH (n=30) and PLWH without any known vascular pathology (n=9). Among aneurysm patients, 13 were PLWH and 17 were HIV-negative. An Olink Target 96 Inflammation panel was used to quantify inflammatory proteins.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We assessed inflammatory profiles in cerebrospinal fluid (CSF) using Olink proteomics in individuals with ruptured anterior communicating artery aneurysms, with and without HIV infection. Among 68 detectable inflammatory proteins, 43 were significantly upregulated in participants with aneurysms (n=30) compared to people living with HIV (PLWH) without aneurysm (n=9). A similar inflammatory signature was observed in HIV-negative aneurysm participants (n=17) and PLWH with aneurysm (n=13), with no significant differences between these two groups. Interleukin-6 (IL-6) was the most upregulated protein across all aneurysm to non-aneurysm comparisons. These findings suggest that aneurysm rupture is associated with a strong CSF inflammatory response, largely independent of HIV status.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Ruptured intracranial aneurysm is associated with strong upregulation of inflammatory proteins in the CSF. This inflammatory response appears largely independent of HIV infection.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.09.30.25337004","type":"posted-content","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T19:55:20Z","timestamp":1759434920000},"source":"Crossref","is-referenced-by-count":0,"title":["Cerebrospinal Fluid Proteomics Reveals Inflammatory Activation in Aneurysmal Subarachnoid Hemorrhage Irrespective of HIV Status"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0391-8496","authenticated-orcid":false,"given":"Rohen","family":"Harrichandparsad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8204-0182","authenticated-orcid":false,"given":"Gil","family":"Lustig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Sviridchik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zesuliwe","family":"Jule","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7498-2391","authenticated-orcid":false,"given":"Mallory","family":"Bernstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1148-2650","authenticated-orcid":false,"given":"Kajal","family":"Reedoy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8330-2853","authenticated-orcid":false,"given":"Yashica","family":"Ganga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afrah","family":"Khairallah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9698-016X","authenticated-orcid":false,"given":"Farina","family":"Karim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinod B.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3103-8488","authenticated-orcid":false,"given":"Ahmed Iqbal","family":"Bhigjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1976-6228","authenticated-orcid":false,"given":"Duncan","family":"Royston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khadija","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8571-2004","authenticated-orcid":false,"given":"Alex","family":"Sigal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"issue":"4","key":"2025100421500558000_2025.09.30.25337004v1.1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1177\/1591019915581789","article-title":"Aneurysmal vasculopathy in human-acquired immunodeficiency virus-infected adults: Imaging case series and review of the literature","volume":"21","year":"2015","journal-title":"Interventional Neuroradiology"},{"issue":"6","key":"2025100421500558000_2025.09.30.25337004v1.2","doi-asserted-by":"crossref","first-page":"107127","DOI":"10.1016\/j.jstrokecerebrovasdis.2023.107127","article-title":"Characteristics and evolution of cerebral aneurysms among adults living with HIV: A retrospective, longitudinal case series","volume":"32","year":"2023","journal-title":"Journal of Stroke and Cerebrovascular Diseases"},{"key":"2025100421500558000_2025.09.30.25337004v1.3","doi-asserted-by":"publisher","DOI":"10.1007\/s10143-004-0371-4"},{"key":"2025100421500558000_2025.09.30.25337004v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.84"},{"key":"2025100421500558000_2025.09.30.25337004v1.5","doi-asserted-by":"publisher","DOI":"10.3171\/2009.10.JNS09759"},{"key":"2025100421500558000_2025.09.30.25337004v1.6","doi-asserted-by":"crossref","unstructured":"Metaxa E , Tremmel M , Xiang J , Kolega J , Mandelbaum M , Siddiqui A , et al. High Wall Shear Stress and Positive Wall Shear Stress Gradient Trigger the Initiation of Intracranial Aneurysms. ASME 2009 Summer Bioengineering Conference, Parts A and B 2009. p. 523\u20134.","DOI":"10.1115\/SBC2009-206395"},{"issue":"6","key":"2025100421500558000_2025.09.30.25337004v1.7","doi-asserted-by":"crossref","first-page":"108304","DOI":"10.1016\/j.jstrokecerebrovasdis.2025.108304","article-title":"Early changes in inflammation-related proteins in the cerebrospinal fluid and plasma of patients with aneurysmal subarachnoid hemorrhage","volume":"34","year":"2025","journal-title":"J Stroke Cerebrovasc Dis"},{"key":"2025100421500558000_2025.09.30.25337004v1.8","doi-asserted-by":"crossref","first-page":"105374","DOI":"10.1016\/j.jprot.2025.105374","article-title":"A comprehensive proteomic analysis reveals novel inflammatory biomarkers in intracranial aneurysms","volume":"313","year":"2025","journal-title":"J Proteomics"},{"key":"2025100421500558000_2025.09.30.25337004v1.9","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis326"},{"issue":"3","key":"2025100421500558000_2025.09.30.25337004v1.10","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1254\/jjp.88.227","article-title":"Cerebrovascular Inflammation Following Subarachnoid Hemorrhage","volume":"88","year":"2002","journal-title":"The Japanese Journal of Pharmacology"},{"key":"2025100421500558000_2025.09.30.25337004v1.11","doi-asserted-by":"crossref","unstructured":"Chai C-Z , Ho U-C , Kuo L-T. Systemic Inflammation after Aneurysmal Subarachnoid Hemorrhage. International Journal of Molecular Sciences [Internet]. 2023; 24(13).","DOI":"10.3390\/ijms241310943"},{"issue":"12","key":"2025100421500558000_2025.09.30.25337004v1.12","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1007\/s00134-006-0408-y","article-title":"Sympathetic activation and inflammatory response in patients with subarachnoid haemorrhage","volume":"32","year":"2006","journal-title":"Intensive Care Medicine"},{"key":"2025100421500558000_2025.09.30.25337004v1.13","doi-asserted-by":"crossref","unstructured":"Karim F , Gazy I , Cele S , Zungu Y , Krause R , Bernstein M , et al. HIV status alters disease severity and immune cell responses in Beta variant SARS-CoV-2 infection wave. 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