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FeoB is the main component of this system, and its function is regulated by the soluble cytosolic domain, termed NFeoB. We have recently begun to define the structure and the mechanism of the Feo system from the bacterium\n                  <jats:italic>Vibrio cholerae<\/jats:italic>\n                  , the causative agent of the disease cholera. However, major structural gaps in our understanding of the nucleotide-promiscuous\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  NFeoB still exist. In this work, we have determined several new X-ray crystal structures that reveal distinct snapshots of the\n                  <jats:italic>Vc<\/jats:italic>\n                  NFeoB domain in uncommon and unprecedented states, ultimately illuminating the full catalytic cycle of this NTPase. This work reveals important functional features of\n                  <jats:italic>Vc<\/jats:italic>\n                  NFeoB that may be leveraged and ultimately targeted to prevent the infectivity and the spread of cholera.\n                <\/jats:p>\n                <jats:sec id=\"s7f\">\n                  <jats:title>Graphical Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"680749v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                    <\/jats:fig>\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.10.06.680749","type":"posted-content","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T02:35:15Z","timestamp":1759804515000},"source":"Crossref","is-referenced-by-count":0,"title":["The structure of the full catalytic cycle of\n                  <i>Vibrio cholerae<\/i>\n                  NFeoB"],"prefix":"10.64898","author":[{"given":"Kate","family":"Magante","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candice M.","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9332-8683","authenticated-orcid":false,"given":"Aaron T.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"2","key":"2025100911401157000_2025.10.06.680749v1.1","first-page":"273","volume":"40","year":"2014","journal-title":"Front. 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