{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:33:40Z","timestamp":1789450420170,"version":"build-2803163510"},"posted":{"date-parts":[[2025,10,9]]},"group-title":"Developmental Biology","reference-count":80,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["DP2HD111708"],"award-info":[{"award-number":["DP2HD111708"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R00HD101021"],"award-info":[{"award-number":["R00HD101021"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Robertson Stem Cell Investigator"],"award-info":[{"award-number":["Robertson Stem Cell Investigator"]}],"id":[{"id":"https:\/\/ror.org\/03n2a3p06","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/006w34k90","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/0550wc655","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/032atxq54","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014185","name":"Searle Scholars Program","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014185","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01gd7b947","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/033km2469","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/05j95n956","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"U.S. National Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>During menstruation, an inner layer of the endometrium is selectively shed, while an outer, progenitor-containing layer is preserved to support repeated regeneration. Progress in understanding this compartmentalization has been hindered by the lack of suitable animal models, as mice and rats do not menstruate. Here, we present transgenic mouse models that recapitulate the key anatomical, functional, and transcriptional features of human menstruation through targeted chemogenetic activation of premenstrual differentiation. Using single-cell spatial transcriptomics, we define a new paradigm for spatially regulated fibroblast differentiation that drives pre-menstrual endometrial layering and ultimately determines the extent of tissue shedding. Our results revise a century-old view of endometrial shedding and regeneration and establish new transgenic mice as powerful tools to advance menstruation research.<\/jats:p>","DOI":"10.1101\/2025.10.08.681007","type":"posted-content","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:35:21Z","timestamp":1760049321000},"source":"Crossref","is-referenced-by-count":7,"title":["Induction of menstruation in mice reveals the regulation of menstrual shedding"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4720-7944","authenticated-orcid":false,"given":"\u00c7a\u011fr\u0131","family":"\u00c7evrim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3839-1962","authenticated-orcid":false,"given":"Nicholas J.","family":"Hilgert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5728-7409","authenticated-orcid":false,"given":"Aellah M.","family":"Kaage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5411-2807","authenticated-orcid":false,"given":"Andrew J.C.","family":"Russell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5800-5443","authenticated-orcid":false,"given":"Allison E.","family":"Goldstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3842-3431","authenticated-orcid":false,"given":"Claire J.","family":"Ang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6906-1571","authenticated-orcid":false,"given":"Jaina L.R.","family":"Gable","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9392-0062","authenticated-orcid":false,"given":"Laura E.","family":"Bagamery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8512-4620","authenticated-orcid":false,"given":"Ana","family":"Breznik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6141-3704","authenticated-orcid":false,"given":"Daniela J.","family":"Di Bella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-1366","authenticated-orcid":false,"given":"Mustafa","family":"Talay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7921-0155","authenticated-orcid":false,"given":"Jingyu","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1980-6840","authenticated-orcid":false,"given":"Kathleen E.","family":"O\u2019Neill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2308-3649","authenticated-orcid":false,"given":"Fei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6676-4706","authenticated-orcid":false,"given":"Sean R.","family":"Eddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6283-9168","authenticated-orcid":false,"given":"Kara L.","family":"McKinley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025101309000630000_2025.10.08.681007v1.1","doi-asserted-by":"crossref","unstructured":"Akhmedov, D. , Mendoza-Rodriguez, M. G. , Rajendran, K. , Rossi, M. , Wess, J. and Berdeaux, R. (2017). Gs-DREADD Knock-In Mice for Tissue-Specific, Temporal Stimulation of Cyclic AMP Signaling. Mol. Cell. Biol. 37.","DOI":"10.1128\/MCB.00584-16"},{"key":"2025101309000630000_2025.10.08.681007v1.2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1990.9999"},{"key":"2025101309000630000_2025.10.08.681007v1.3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-011723-021442"},{"key":"2025101309000630000_2025.10.08.681007v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17565-x"},{"key":"2025101309000630000_2025.10.08.681007v1.5","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/ar.1090930107","article-title":"The day to day level of estrogen and progestin throughout pregnancy and pseudopregnancy in the mouse","volume":"93","year":"1945","journal-title":"Anat. Rec"},{"key":"2025101309000630000_2025.10.08.681007v1.6","doi-asserted-by":"crossref","unstructured":"Baldarelli, R. M. , Smith, C. L. , Ringwald, M. , Richardson, J. E. , Bult, C. J. and Mouse Genome Informatics Group (2024). Mouse Genome Informatics: an integrated knowledgebase system for the laboratory mouse. Genetics 227.","DOI":"10.1093\/genetics\/iyae031"},{"key":"2025101309000630000_2025.10.08.681007v1.7","doi-asserted-by":"publisher","DOI":"10.1145\/235815.235821"},{"key":"2025101309000630000_2025.10.08.681007v1.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"2025101309000630000_2025.10.08.681007v1.9","first-page":"65","article-title":"Julia: A fresh approach to numerical computing","volume":"59","year":"2017","journal-title":"SIAM Rev. Soc. Ind. Appl. Math"},{"key":"2025101309000630000_2025.10.08.681007v1.10","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.016550"},{"key":"2025101309000630000_2025.10.08.681007v1.11","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1071\/BI9820063","article-title":"Lectins, calcium ionophore A23187 and peanut oil as deciduogenic agents in the uterus of pseudopregnant mice: Effects of tranylcypromine, indomethacin, iproniazid and propanolol","volume":"35","year":"1982","journal-title":"Aust. J. Biol. Sci"},{"key":"2025101309000630000_2025.10.08.681007v1.12","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"2025101309000630000_2025.10.08.681007v1.13","first-page":"811537","article-title":"Endometrial stem\/progenitor cells-their role in endome-trial repair and regeneration","volume":"3","year":"2021","journal-title":"Front. Reprod. Health"},{"key":"2025101309000630000_2025.10.08.681007v1.14","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00031.2019"},{"key":"2025101309000630000_2025.10.08.681007v1.15","doi-asserted-by":"publisher","DOI":"10.3724\/SP.J.1087.2009.02191"},{"key":"2025101309000630000_2025.10.08.681007v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2019.07.004"},{"key":"2025101309000630000_2025.10.08.681007v1.17","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1265"},{"key":"2025101309000630000_2025.10.08.681007v1.18","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1093\/biolre\/ioad012","article-title":"iCre recombinase expressed in the anti-M\u00fcllerian hormone receptor 2 gene causes global genetic modification in the mouse","volume":"108","year":"2023","journal-title":"Biol. Reprod"},{"key":"2025101309000630000_2025.10.08.681007v1.19","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201100099"},{"key":"2025101309000630000_2025.10.08.681007v1.20","doi-asserted-by":"publisher","DOI":"10.1210\/endo-83-5-933"},{"key":"2025101309000630000_2025.10.08.681007v1.21","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1000295"},{"key":"2025101309000630000_2025.10.08.681007v1.22","unstructured":"Gayoso, A. and Shor, J. (2020). DoubletDetection: doubletdetection v3.0. Zenodo."},{"key":"2025101309000630000_2025.10.08.681007v1.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.03.011"},{"key":"2025101309000630000_2025.10.08.681007v1.24","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3971"},{"key":"2025101309000630000_2025.10.08.681007v1.25","doi-asserted-by":"crossref","first-page":"346","DOI":"10.2527\/jas1952.112346x","article-title":"The causes of bovine metestrous bleeding","volume":"11","year":"1952","journal-title":"J. Anim. Sci"},{"key":"2025101309000630000_2025.10.08.681007v1.26","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2649-2"},{"key":"2025101309000630000_2025.10.08.681007v1.27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1049"},{"key":"2025101309000630000_2025.10.08.681007v1.28","doi-asserted-by":"publisher","DOI":"10.1038\/S41576-023-00586-W"},{"key":"2025101309000630000_2025.10.08.681007v1.29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044285"},{"key":"2025101309000630000_2025.10.08.681007v1.30","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.mce.2014.02.005","article-title":"Cyclic adenosine monophosphate-induced argininosuccinate synthase 1 expression is essential during mouse decidualization","volume":"388","year":"2014","journal-title":"Mol. Cell. Endocrinol"},{"key":"2025101309000630000_2025.10.08.681007v1.31","doi-asserted-by":"publisher","DOI":"10.1038\/ng1003"},{"key":"2025101309000630000_2025.10.08.681007v1.32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1440-169X.1989.00023.x","article-title":"Expression Pattern of E- and P-Cadherin in Mouse Embryos and Uteri during the Periimplantation Period: (implantation\/mouse embryo\/-cell adhesion molecules E-cadherin\/P-cadherin): (implantation\/mouse embryo\/cell adhesion molecules E-cadherin\/P-cadherin)","volume":"31","year":"1989","journal-title":"Dev. Growth Differ"},{"key":"2025101309000630000_2025.10.08.681007v1.33","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0358-2"},{"key":"2025101309000630000_2025.10.08.681007v1.34","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0716"},{"key":"2025101309000630000_2025.10.08.681007v1.35","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009-1334"},{"key":"2025101309000630000_2025.10.08.681007v1.36","doi-asserted-by":"crossref","unstructured":"Kirkwood, P. M. , Gibson, D. A. , Smith, J. R. , Wilson-Kanamori, J. R. , Kelepouri, O. , Esnal-Zufiaurre, A. , Dobie, R. , Henderson, N. C. and Saunders, P. T. K. (2021). Single\u2010cell RNA sequencing redefines the mesenchymal cell landscape of mouse endometrium. FASEB J. 35,.","DOI":"10.1096\/fj.202002123R"},{"key":"2025101309000630000_2025.10.08.681007v1.37","doi-asserted-by":"crossref","unstructured":"Kirkwood, P. M. , Gibson, D. A. , Shaw, I. , Dobie, R. , Kelepouri, O. , Henderson, N. C. and Saunders, P. T.K. (2022). Single-cell RNA sequencing and lineage tracing confirm mesenchyme to epithelial transformation (MET) contributes to repair of the endometrium at menstruation. Elife 11,.","DOI":"10.7554\/eLife.77663"},{"key":"2025101309000630000_2025.10.08.681007v1.38","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.69.066138"},{"key":"2025101309000630000_2025.10.08.681007v1.39","doi-asserted-by":"crossref","unstructured":"Leroy, F. and Lejeune, B. (1981). The uterine epithelium as a transducer for the triggering of decidualization in the rat. In Cellular and Molecular Aspects of Implantation, pp. 443\u2013445. Boston, MA: Springer US.","DOI":"10.1007\/978-1-4613-3180-3_47"},{"key":"2025101309000630000_2025.10.08.681007v1.40","doi-asserted-by":"crossref","first-page":"4463","DOI":"10.3892\/mmr.2020.11567","article-title":"Mouse model of menstruation: An indispensable tool to investigate the mechanisms of menstruation and gynaecological diseases (Review)","volume":"22","year":"2020","journal-title":"Mol. Med. Rep"},{"key":"2025101309000630000_2025.10.08.681007v1.41","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-5-60"},{"key":"2025101309000630000_2025.10.08.681007v1.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-018-0229-21"},{"key":"2025101309000630000_2025.10.08.681007v1.43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02375-6"},{"key":"2025101309000630000_2025.10.08.681007v1.44","doi-asserted-by":"publisher","DOI":"10.21105\/joss.00861"},{"key":"2025101309000630000_2025.10.08.681007v1.45","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10268"},{"key":"2025101309000630000_2025.10.08.681007v1.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2021.01.007"},{"key":"2025101309000630000_2025.10.08.681007v1.47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.15.6861"},{"key":"2025101309000630000_2025.10.08.681007v1.48","doi-asserted-by":"publisher","DOI":"10.1210\/en.138.12.5511"},{"key":"2025101309000630000_2025.10.08.681007v1.49","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0435"},{"key":"2025101309000630000_2025.10.08.681007v1.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpc.2010.04.018"},{"key":"2025101309000630000_2025.10.08.681007v1.51","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1242989"},{"key":"2025101309000630000_2025.10.08.681007v1.52","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod17.4.549"},{"key":"2025101309000630000_2025.10.08.681007v1.53","unstructured":"Rasmussen, C. E. and Williams, C. K. I. (2019). Gaussian processes for machine learning. London, England: MIT Press."},{"key":"2025101309000630000_2025.10.08.681007v1.54","doi-asserted-by":"crossref","unstructured":"Rogers, L. M. , Lash, G. E. , Anderson, G. M. and Girling, J. E. (2025). Modelling menstruation in the common mouse: a narrative review. Reprod. Fertil. Dev. 37,.","DOI":"10.1071\/RD25055"},{"key":"2025101309000630000_2025.10.08.681007v1.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.01.040"},{"key":"2025101309000630000_2025.10.08.681007v1.56","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032922"},{"key":"2025101309000630000_2025.10.08.681007v1.57","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06837-4"},{"key":"2025101309000630000_2025.10.08.681007v1.58","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1010097"},{"key":"2025101309000630000_2025.10.08.681007v1.59","doi-asserted-by":"publisher","DOI":"10.1006\/bmme.1996.0013"},{"key":"2025101309000630000_2025.10.08.681007v1.60","doi-asserted-by":"crossref","unstructured":"Salamonsen, L. A. , Hutchison, J. C. and Gargett, C. E. (2021). Cyclical endometrial repair and regeneration. Development 148,.","DOI":"10.1242\/dev.199577"},{"key":"2025101309000630000_2025.10.08.681007v1.61","doi-asserted-by":"publisher","DOI":"10.1293\/tox.2016-0021"},{"key":"2025101309000630000_2025.10.08.681007v1.62","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"2025101309000630000_2025.10.08.681007v1.63","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/BF01708334","article-title":"Zur Morphologie und Histologie des Menstruationszyklus","volume":"121","year":"1923","journal-title":"Arch. Gynak"},{"key":"2025101309000630000_2025.10.08.681007v1.64","unstructured":"Serv\u00e9n, D. and Brummitt, C. (2018). pyGAM: Generalized Additive Models in Python. Zenodo."},{"key":"2025101309000630000_2025.10.08.681007v1.65","doi-asserted-by":"crossref","first-page":"1027164","DOI":"10.3389\/fendo.2022.1027164","article-title":"A role for steroid 5 alpha-reductase 1 in vascular remodeling during endometrial decidualization","volume":"13","year":"2022","journal-title":"Front. Endocrinol. (Lausanne)"},{"key":"2025101309000630000_2025.10.08.681007v1.66","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-022-03711-z"},{"key":"2025101309000630000_2025.10.08.681007v1.67","doi-asserted-by":"crossref","unstructured":"Tarashansky, A. J. , Musser, J. M. , Khariton, M. , Li, P. , Arendt, D. , Quake, S. R. and Wang, B. (2021). Mapping single-cell atlases throughout Metazoa unravels cell type evolution. Elife 10,.","DOI":"10.7554\/eLife.66747"},{"key":"2025101309000630000_2025.10.08.681007v1.68","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41695-z"},{"key":"2025101309000630000_2025.10.08.681007v1.69","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3516"},{"key":"2025101309000630000_2025.10.08.681007v1.70","doi-asserted-by":"publisher","DOI":"10.21105\/joss.04371"},{"key":"2025101309000630000_2025.10.08.681007v1.71","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0686-2"},{"key":"2025101309000630000_2025.10.08.681007v1.72","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1040-z"},{"key":"2025101309000630000_2025.10.08.681007v1.73","doi-asserted-by":"crossref","first-page":"e2412854","DOI":"10.1001\/jamanetworkopen.2024.12854","article-title":"Menarche and time to cycle regularity among individuals born between 1950 and 2005 in the US","volume":"7","year":"2024","journal-title":"JAMA Netw. Open"},{"key":"2025101309000630000_2025.10.08.681007v1.74","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.01.021"},{"key":"2025101309000630000_2025.10.08.681007v1.75","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1382-0"},{"key":"2025101309000630000_2025.10.08.681007v1.76","first-page":"435","article-title":"Abnormal uterine bleeding in premenopausal women","volume":"99","year":"2019","journal-title":"Am. Fam. Physician"},{"key":"2025101309000630000_2025.10.08.681007v1.77","doi-asserted-by":"crossref","first-page":"7023","DOI":"10.1111\/jcmm.15372","article-title":"Bmp2 regulates Serpinb6b expression via cAMP\/P-KA\/Wnt4 pathway during uterine decidualization","volume":"24","year":"2020","journal-title":"J. Cell. Mol. Med"},{"key":"2025101309000630000_2025.10.08.681007v1.78","doi-asserted-by":"crossref","unstructured":"Zappia, L. and Oshlack, A. (2018). Clustering trees: a visualization for evaluating clusterings at multiple resolutions. Gigascience 7.","DOI":"10.1093\/gigascience\/giy083"},{"key":"2025101309000630000_2025.10.08.681007v1.79","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14049"},{"key":"2025101309000630000_2025.10.08.681007v1.80","doi-asserted-by":"publisher","DOI":"10.1002\/DVG.22949"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.10.08.681007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:46:18Z","timestamp":1768470378000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.10.08.681007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,9]]},"references-count":80,"URL":"https:\/\/doi.org\/10.1101\/2025.10.08.681007","relation":{},"subject":[],"published":{"date-parts":[[2025,10,9]]},"subtype":"preprint"}}