{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:33:51Z","timestamp":1785407631335,"version":"3.56.0"},"posted":{"date-parts":[[2025,10,8]]},"group-title":"Developmental Biology","reference-count":0,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Kansas Idea Network of Biomedical Research Excellence","award":["P20 GM103418"],"award-info":[{"award-number":["P20 GM103418"]}]},{"name":"Program of the Joint Usage\/Research Center for Developmental Medicine and High Depth Omics, IMEG, Kumamoto University"},{"id":[{"id":"https:\/\/ror.org\/03vpdge44","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NIH","award":["HD115834"],"award-info":[{"award-number":["HD115834"]}]},{"name":"NIH","award":["HD104071"],"award-info":[{"award-number":["HD104071"]}]},{"name":"NIH","award":["HD020676"],"award-info":[{"award-number":["HD020676"]}]},{"name":"NIH","award":["HD105734"],"award-info":[{"award-number":["HD105734"]}]},{"name":"NIH","award":["HD112559"],"award-info":[{"award-number":["HD112559"]}]},{"id":[{"id":"https:\/\/ror.org\/01thz9g52","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,11]]},"abstract":"<jats:p>The expansion and differentiation of trophoblast stem (TS) cells are critical for defining fundamental properties of the placenta. Specialized trophoblast cells exit the placenta and enter and transform the uterus, including restructuring uterine spiral arteries. In the human these cells are named extravillous trophoblast (EVT) cells, whereas in the rat they are termed invasive trophoblast cells. Mechanisms governing invasive trophoblast cell differentiation remain poorly understood. We investigated peroxisome proliferator-activated receptor gamma (PPARG) as a potential regulator of EVT\/invasive trophoblast cell development. In first trimester human placentas, PPARG was expressed in the EVT cell column and increased in amount as human TS cells differentiated into EVT cells. PPARG disruption impaired EVT cell differentiation. Rat invasive trophoblast cells similarly expressed PPARG. Conditional inactivation of PPARG within rat invasive trophoblast cells was used to assess the in vivo role of PPARG on the uterine-placental interface. PPARG was established as an essential cell-autonomous regulator of the invasive trophoblast cells. In conclusion, PPARG is a conserved regulator of placentation and is essential for directing trophoblast cell-guided uterine transformation.<\/jats:p>","DOI":"10.1101\/2025.10.08.681177","type":"posted-content","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T03:45:10Z","timestamp":1759981510000},"source":"Crossref","is-referenced-by-count":2,"title":["PPARG directs trophoblast cell fate and establishment of the uterine-placental interface"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3540-4411","authenticated-orcid":false,"given":"Esteban M","family":"Dominguez","sequence":"first","affiliation":[{"name":"University of Kansas Medical Center;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6810-6475","authenticated-orcid":false,"given":"Ayelen Moreno","family":"Irusta","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-4591","authenticated-orcid":false,"given":"KHURSHEED","family":"IQBAL","sequence":"additional","affiliation":[{"name":"UNIVERSITY OF KANSAS MEDICAL CENTER;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keting","family":"Chen","sequence":"additional","affiliation":[{"name":"Iowa State University;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Finlinson","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Parrish","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Okae","sequence":"additional","affiliation":[{"name":"Kumamoto University;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Arima","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geetu","family":"Tuteja","sequence":"additional","affiliation":[{"name":"Iowa State University;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J","family":"Soares","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.10.08.681177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:30:18Z","timestamp":1773286218000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.10.08.681177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,8]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1101\/2025.10.08.681177","relation":{},"subject":[],"published":{"date-parts":[[2025,10,8]]},"subtype":"preprint"}}