{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:17:52Z","timestamp":1785406672484,"version":"3.56.0"},"posted":{"date-parts":[[2025,10,14]]},"group-title":"Immunology","reference-count":105,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["AI110508"],"award-info":[{"award-number":["AI110508"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AI50740"],"award-info":[{"award-number":["AI50740"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AI53413"],"award-info":[{"award-number":["AI53413"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AI52476"],"award-info":[{"award-number":["AI52476"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CA013148"],"award-info":[{"award-number":["CA013148"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AI027767"],"award-info":[{"award-number":["AI027767"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Systemically administered influenza vaccines provide strain-limited protection, while influenza infection of the respiratory epithelium supports development of lung resident memory B and T cells and more broadly reactive antibody responses. To test whether local antigen delivery is critical for establishing broad immunity, we directly compared respiratory tract and systemic delivery of influenza antigens to mice and hamsters using a replication-deficient adenovirus serotype 5 vector (Ad5[E1-,E2b-,E3-]). Both immunization routes elicited antibody responses in the lower respiratory tract and antigen-specific B and T cells in the draining lymph node. However, only intranasal immunization established lung-resident memory B and T cells, induced IgA responses in the upper respiratory tract directly at the site of viral entry and supported generation of IgA and IgG antibodies that bound antigenically drifted and distantly related influenza strains, including those of avian origin. Intranasal immunization accelerated viral clearance following heterologous virus challenge and was associated with limited pulmonary inflammation and fibrosis. Thus, intranasal immunization with the immunologically stealthy Ad5[E1-,E2b-,E3-] platform supports respiratory and systemic immunity to divergent influenza strains in the absence of overt lung immunopathology, suggesting that local antigen delivery may be key to development of more broadly protective \u201cuniversal\u201d flu vaccines.<\/jats:p>","DOI":"10.1101\/2025.10.13.682194","type":"posted-content","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:10:48Z","timestamp":1760487048000},"source":"Crossref","is-referenced-by-count":1,"title":["Mucosal delivery of influenza antigens using a replication deficient adenovirus supports broadly reactive antibody responses and heterologous viral immunity in the respiratory tract of animals"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-4115","authenticated-orcid":false,"given":"Michael D.","family":"Schultz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-0662","authenticated-orcid":false,"given":"Davide","family":"Botta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1882-2534","authenticated-orcid":false,"given":"Aaron","family":"Silva-Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4271-2774","authenticated-orcid":false,"given":"Isabella R.","family":"Cheatwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6337-1599","authenticated-orcid":false,"given":"Davies","family":"Kalange","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0797-7442","authenticated-orcid":false,"given":"Sarah M.","family":"Terapane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron C. K.","family":"Lucander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3096-3663","authenticated-orcid":false,"given":"Jobaida","family":"Akther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Cockerham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William O.","family":"Stover","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1879-8776","authenticated-orcid":false,"given":"John A.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9260-4079","authenticated-orcid":false,"given":"Douglas M.","family":"Fox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8573-5668","authenticated-orcid":false,"given":"Francisco","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6939-9572","authenticated-orcid":false,"suffix":"Jr.","given":"Sixto M.","family":"Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6621-6096","authenticated-orcid":false,"given":"Jeremy B.","family":"Foote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0780-9470","authenticated-orcid":false,"given":"Kevin S.","family":"Harrod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0643-0311","authenticated-orcid":false,"given":"Troy D.","family":"Randall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian E.","family":"Rice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth S.","family":"Gabitzsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3083-1246","authenticated-orcid":false,"given":"Frances E.","family":"Lund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025101705301359000_2025.10.13.682194v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.03.046"},{"key":"2025101705301359000_2025.10.13.682194v1.2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwp173"},{"issue":"2","key":"2025101705301359000_2025.10.13.682194v1.3","doi-asserted-by":"crossref","first-page":"020421","DOI":"10.7189\/jogh.09.020421","article-title":"Global mortality associated with seasonal influenza epidemics: New burden estimates and predictors from the GLaMOR Project","volume":"9","year":"2019","journal-title":"J Glob Health"},{"key":"2025101705301359000_2025.10.13.682194v1.4","doi-asserted-by":"crossref","first-page":"A6","DOI":"10.1016\/j.vaccine.2020.09.048","article-title":"The disease burden of influenza beyond respiratory illness","volume":"39","year":"2021","journal-title":"Vaccine"},{"key":"2025101705301359000_2025.10.13.682194v1.5","doi-asserted-by":"crossref","unstructured":"Chen, J.R. , et al., Better influenza vaccines: an industry perspective. Journal of Biomedical Science, 2020. 27(1).","DOI":"10.1186\/s12929-020-0626-6"},{"key":"2025101705301359000_2025.10.13.682194v1.6","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1146\/annurev-virology-010320-044746","article-title":"Challenges of Making Effective Influenza Vaccines","volume":"7","year":"2020","journal-title":"Annual Review of Virology"},{"issue":"12 Suppl 2","key":"2025101705301359000_2025.10.13.682194v1.7","first-page":"S405","article-title":"Influenza Vaccines: Successes and Continuing Challenges","volume":"224","year":"2021","journal-title":"J Infect Dis"},{"issue":"2","key":"2025101705301359000_2025.10.13.682194v1.8","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1093\/infdis\/jiaa757","article-title":"Influenza Vaccine Failure Associated With Age and Immunosuppression","volume":"224","year":"2021","journal-title":"Journal of Infectious Diseases"},{"issue":"4","key":"2025101705301359000_2025.10.13.682194v1.9","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1093\/cid\/ciae491","article-title":"Influenza Vaccine Effectiveness Against Illness and Asymptomatic Infection in 2022-2023: A Prospective Cohort Study","volume":"80","year":"2025","journal-title":"Clin Infect Dis"},{"key":"2025101705301359000_2025.10.13.682194v1.10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1118979109"},{"key":"2025101705301359000_2025.10.13.682194v1.11","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03509-12"},{"key":"2025101705301359000_2025.10.13.682194v1.12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025797"},{"key":"2025101705301359000_2025.10.13.682194v1.13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200039109"},{"key":"2025101705301359000_2025.10.13.682194v1.14","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101352"},{"key":"2025101705301359000_2025.10.13.682194v1.15","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0143-6"},{"key":"2025101705301359000_2025.10.13.682194v1.16","first-page":"47","article-title":"Advances in influenza vaccination","volume":"6","year":"2014","journal-title":"F1000Prime Rep"},{"key":"2025101705301359000_2025.10.13.682194v1.17","doi-asserted-by":"crossref","unstructured":"McMillan, C.L.D. , et al., The Next Generation of Influenza Vaccines: Towards a Universal Solution. Vaccines (Basel), 2021. 9(1).","DOI":"10.3390\/vaccines9010026"},{"key":"2025101705301359000_2025.10.13.682194v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.04.013"},{"key":"2025101705301359000_2025.10.13.682194v1.19","first-page":"39","article-title":"Role of mucosal immunity in influenza","volume":"115","year":"2003","journal-title":"Dev Biol (Basel"},{"key":"2025101705301359000_2025.10.13.682194v1.20","doi-asserted-by":"crossref","unstructured":"Wang, T. , F. Wei , and J. Liu , Emerging Role of Mucosal Vaccine in Preventing Infection with Avian Influenza A Viruses. Viruses, 2020. 12(8).","DOI":"10.3390\/v12080862"},{"key":"2025101705301359000_2025.10.13.682194v1.21","doi-asserted-by":"crossref","unstructured":"de Fays, C. , et al., Secretory Immunoglobulin A Immunity in Chronic Obstructive Respiratory Diseases. Cells, 2022. 11(8).","DOI":"10.3390\/cells11081324"},{"key":"2025101705301359000_2025.10.13.682194v1.22","doi-asserted-by":"crossref","first-page":"105823","DOI":"10.1016\/j.antiviral.2024.105823","article-title":"Unmasking the potential of secretory IgA and its pivotal role in protection from respiratory viruses","volume":"223","year":"2024","journal-title":"Antiviral Res"},{"key":"2025101705301359000_2025.10.13.682194v1.23","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abj5129"},{"key":"2025101705301359000_2025.10.13.682194v1.24","doi-asserted-by":"crossref","unstructured":"Booth, J.S. and F.R. Toapanta , B and T Cell Immunity in Tissues and Across the Ages. Vaccines (Basel), 2021. 9(1).","DOI":"10.3390\/vaccines9010024"},{"key":"2025101705301359000_2025.10.13.682194v1.25","doi-asserted-by":"crossref","unstructured":"Nelson, S.A. and A.J. Sant , Potentiating Lung Mucosal Immunity Through Intranasal Vaccination. Frontiers in Immunology, 2021. 12.","DOI":"10.3389\/fimmu.2021.808527"},{"key":"2025101705301359000_2025.10.13.682194v1.26","doi-asserted-by":"crossref","first-page":"738955","DOI":"10.3389\/fimmu.2021.738955","article-title":"Pulmonary-Resident Memory Lymphocytes: Pivotal Orchestrators of Local Immunity Against Respiratory Infections","volume":"12","year":"2021","journal-title":"Front Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.27","doi-asserted-by":"crossref","first-page":"1433452","DOI":"10.3389\/fimmu.2024.1433452","article-title":"Mucosal immunity in COVID-19: a comprehensive review","volume":"15","year":"2024","journal-title":"Front Immunol"},{"issue":"12","key":"2025101705301359000_2025.10.13.682194v1.28","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1016\/j.immuni.2024.10.003","article-title":"Maintenance and functional regulation of immune memory to COVID-19 vaccines in tissues","volume":"57","year":"2024","journal-title":"Immunity"},{"key":"2025101705301359000_2025.10.13.682194v1.29","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42433-w"},{"key":"2025101705301359000_2025.10.13.682194v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.01.003"},{"key":"2025101705301359000_2025.10.13.682194v1.31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.ai.2022.08.001","article-title":"Development and function of tissue-resident memory B cells","volume":"155","year":"2022","journal-title":"Adv Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.32","doi-asserted-by":"crossref","first-page":"1216402","DOI":"10.3389\/fimmu.2023.1216402","article-title":"Pivotal role of tissue-resident memory lymphocytes in the control of mucosal infections: can mucosal vaccination induce protective tissue-resident memory T and B cells?","volume":"14","year":"2023","journal-title":"Front Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.33","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0260-6"},{"key":"2025101705301359000_2025.10.13.682194v1.34","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0260-6"},{"issue":"6","key":"2025101705301359000_2025.10.13.682194v1.35","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/s41590-025-02156-0","article-title":"Mucosal unadjuvanted booster vaccines elicit local IgA responses by conversion of pre-existing immunity in mice","volume":"26","year":"2025","journal-title":"Nat Immunol"},{"issue":"1","key":"2025101705301359000_2025.10.13.682194v1.36","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1186\/s12951-025-03582-w","article-title":"Targeting heptad repeats and fusion peptide: nanoparticle vaccine elicits mucosal immune response against SARS-CoV-2 variants","volume":"23","year":"2025","journal-title":"J Nanobiotechnology"},{"key":"2025101705301359000_2025.10.13.682194v1.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.08.015"},{"issue":"7","key":"2025101705301359000_2025.10.13.682194v1.38","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1016\/j.immuni.2025.05.021","article-title":"Transcription factor T-bet regulates the maintenance and differentiation potential of lymph node and lung effector memory B cell subsets","volume":"58","year":"2025","journal-title":"Immunity"},{"key":"2025101705301359000_2025.10.13.682194v1.39","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20142284"},{"issue":"1","key":"2025101705301359000_2025.10.13.682194v1.40","doi-asserted-by":"crossref","first-page":"2514356","DOI":"10.1080\/21645515.2025.2514356","article-title":"Developing the next-generation of adenoviral vector vaccines","volume":"21","year":"2025","journal-title":"Hum Vaccin Immunother"},{"issue":"10","key":"2025101705301359000_2025.10.13.682194v1.41","doi-asserted-by":"crossref","first-page":"e1345","DOI":"10.1002\/cti2.1345","article-title":"Adenoviral vector-based platforms for developing effective vaccines to combat respiratory viral infections","volume":"10","year":"2021","journal-title":"Clin Transl Immunology"},{"issue":"1","key":"2025101705301359000_2025.10.13.682194v1.42","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.4110\/in.2021.21.e6","article-title":"Adenovirus Vectors: Excellent Tools for Vaccine Development","volume":"21","year":"2021","journal-title":"Immune Netw"},{"issue":"1","key":"2025101705301359000_2025.10.13.682194v1.43","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/s41541-021-00356-x","article-title":"Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic","volume":"6","year":"2021","journal-title":"NPJ Vaccines"},{"issue":"4","key":"2025101705301359000_2025.10.13.682194v1.44","doi-asserted-by":"crossref","first-page":"100230","DOI":"10.1016\/j.xcrm.2021.100230","article-title":"A single intranasal dose of chimpanzee adenovirus-vectored vaccine protects against SARS-CoV-2 infection in rhesus macaques","volume":"2","year":"2021","journal-title":"Cell Rep Med"},{"key":"2025101705301359000_2025.10.13.682194v1.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.026"},{"key":"2025101705301359000_2025.10.13.682194v1.46","doi-asserted-by":"crossref","first-page":"729837","DOI":"10.3389\/fimmu.2021.729837","article-title":"Dual-Antigen COVID-19 Vaccine Subcutaneous Prime Delivery With Oral Boosts Protects NHP Against SARS-CoV-2 Challenge","volume":"12","year":"2021","journal-title":"Front Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030133"},{"key":"2025101705301359000_2025.10.13.682194v1.48","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.926-933.1998"},{"issue":"50","key":"2025101705301359000_2025.10.13.682194v1.49","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1016\/j.vaccine.2012.09.058","article-title":"Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform","volume":"30","year":"2012","journal-title":"Vaccine"},{"key":"2025101705301359000_2025.10.13.682194v1.50","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-013-1400-3"},{"issue":"40","key":"2025101705301359000_2025.10.13.682194v1.51","doi-asserted-by":"crossref","first-page":"7020","DOI":"10.1016\/j.vaccine.2011.07.073","article-title":"Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine","volume":"29","year":"2011","journal-title":"Vaccine"},{"key":"2025101705301359000_2025.10.13.682194v1.52","doi-asserted-by":"crossref","unstructured":"Risley, C.A. , et al., T-bet expression marks a transcriptionally and functionally distinct population of memory B cells. Journal of Immunology, 2021. 206.","DOI":"10.4049\/jimmunol.206.Supp.114.18"},{"key":"2025101705301359000_2025.10.13.682194v1.53","doi-asserted-by":"crossref","unstructured":"Zens, K.D. , J.K. Chen , and D.L. Farber , Vaccine-generated lung tissue-resident memory T cells provide heterosubtypic protection to influenza infection. JCI Insight, 2016. 1(10).","DOI":"10.1172\/jci.insight.85832"},{"key":"2025101705301359000_2025.10.13.682194v1.54","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-018-0003-x"},{"key":"2025101705301359000_2025.10.13.682194v1.55","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004273"},{"key":"2025101705301359000_2025.10.13.682194v1.56","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.02.111"},{"key":"2025101705301359000_2025.10.13.682194v1.57","doi-asserted-by":"crossref","first-page":"1445771","DOI":"10.3389\/fimmu.2024.1445771","article-title":"Ultrasensitive protein-level detection for respiratory infectious viruses","volume":"15","year":"2024","journal-title":"Front Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.58","doi-asserted-by":"crossref","unstructured":"Klinkhammer, J. , et al., IFN-lambda prevents influenza virus spread from the upper airways to the lungs and limits virus transmission. Elife, 2018. 7.","DOI":"10.7554\/eLife.33354"},{"key":"2025101705301359000_2025.10.13.682194v1.59","doi-asserted-by":"publisher","DOI":"10.1177\/0192623315598399"},{"issue":"4","key":"2025101705301359000_2025.10.13.682194v1.60","first-page":"287","article-title":"A Review of the Comparative Anatomy, Histology, Physiology and Pathology of the Nasal Cavity of Rats, Mice","volume":"153","year":"2015","journal-title":"Dogs and Non-human Primates. Relevance to Inhalation Toxicology and Human Health Risk Assessment. J Comp Pathol"},{"key":"2025101705301359000_2025.10.13.682194v1.61","doi-asserted-by":"publisher","DOI":"10.1080\/10635150490468701"},{"key":"2025101705301359000_2025.10.13.682194v1.62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2006.01.010"},{"key":"2025101705301359000_2025.10.13.682194v1.63","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2009799117"},{"issue":"5","key":"2025101705301359000_2025.10.13.682194v1.64","first-page":"1","article-title":"Hamsters as a Model of Severe Acute Respiratory Syndrome Coronavirus-2","volume":"71","year":"2021","journal-title":"Comparative Medicine"},{"key":"2025101705301359000_2025.10.13.682194v1.65","doi-asserted-by":"crossref","unstructured":"Iwatsuki-Horimoto, K. , et al., Syrian Hamster as an Animal Model for the Study of Human Influenza Virus Infection. Journal of Virology, 2018. 92(4).","DOI":"10.1128\/JVI.01693-17"},{"key":"2025101705301359000_2025.10.13.682194v1.66","doi-asserted-by":"publisher","DOI":"10.3390\/v20801530"},{"key":"2025101705301359000_2025.10.13.682194v1.67","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.13416"},{"key":"2025101705301359000_2025.10.13.682194v1.68","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183070"},{"key":"2025101705301359000_2025.10.13.682194v1.69","first-page":"21","article-title":"Role of iBALT in Respiratory Immunity","volume":"426","year":"2020","journal-title":"Curr Top Microbiol Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.70","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-010-0847-8"},{"key":"2025101705301359000_2025.10.13.682194v1.71","doi-asserted-by":"crossref","first-page":"953088","DOI":"10.3389\/fimmu.2022.953088","article-title":"Resident Memory B Cells in Barrier Tissues","volume":"13","year":"2022","journal-title":"Front Immunol"},{"issue":"14","key":"2025101705301359000_2025.10.13.682194v1.72","doi-asserted-by":"crossref","first-page":"e2416855122","DOI":"10.1073\/pnas.2416855122","article-title":"Lung B cells in ectopic germinal centers undergo affinity maturation","volume":"122","year":"2025","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"2025101705301359000_2025.10.13.682194v1.73","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1038\/s41423-025-01285-8","article-title":"Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph node","volume":"22","year":"2025","journal-title":"Cell Mol Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.74","doi-asserted-by":"crossref","unstructured":"Son, Y.M. , et al., Tissue-resident CD4(+) T helper cells assist the development of protective respiratory B and CD8(+) T cell memory responses. Sci Immunol, 2021. 6(55).","DOI":"10.1126\/sciimmunol.abb6852"},{"key":"2025101705301359000_2025.10.13.682194v1.75","doi-asserted-by":"crossref","unstructured":"Swarnalekha, N. , et al., T resident helper cells promote humoral responses in the lung. Sci Immunol, 2021. 6(55).","DOI":"10.1126\/sciimmunol.abb6808"},{"key":"2025101705301359000_2025.10.13.682194v1.76","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.113869"},{"key":"2025101705301359000_2025.10.13.682194v1.77","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12744"},{"issue":"4","key":"2025101705301359000_2025.10.13.682194v1.78","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1089\/vim.2019.0141","article-title":"Resident Memory B Cells","volume":"33","year":"2020","journal-title":"Viral Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.79","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.06.002"},{"key":"2025101705301359000_2025.10.13.682194v1.80","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109286"},{"key":"2025101705301359000_2025.10.13.682194v1.81","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07748-8"},{"key":"2025101705301359000_2025.10.13.682194v1.82","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abf5314"},{"key":"2025101705301359000_2025.10.13.682194v1.83","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2018.1516553"},{"key":"2025101705301359000_2025.10.13.682194v1.84","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17497-6"},{"key":"2025101705301359000_2025.10.13.682194v1.85","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800671"},{"issue":"1","key":"2025101705301359000_2025.10.13.682194v1.86","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1080\/13696998.2022.2098630","article-title":"Impact of influenza infection on the short- and long-term health of patients with chronic obstructive pulmonary disease","volume":"25","year":"2022","journal-title":"J Med Econ"},{"key":"2025101705301359000_2025.10.13.682194v1.87","doi-asserted-by":"crossref","unstructured":"Suri, C. , et al., Interplay between Lung Diseases and Viral Infections: A Comprehensive Review. Microorganisms, 2024. 12(10).","DOI":"10.3390\/microorganisms12102030"},{"key":"2025101705301359000_2025.10.13.682194v1.88","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2011.41"},{"key":"2025101705301359000_2025.10.13.682194v1.89","first-page":"222","article-title":"Secretory IgA: Designed for Anti-Microbial Defense","volume":"4","year":"2013","journal-title":"Front Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.90","doi-asserted-by":"crossref","unstructured":"Corth\u00e9sy, B ., Multi-faceted functions of secretory IgA at mucosal surfaces. Frontiers in Immunology, 2013. 4.","DOI":"10.3389\/fimmu.2013.00185"},{"key":"2025101705301359000_2025.10.13.682194v1.91","doi-asserted-by":"publisher","DOI":"10.1172\/JCI132005"},{"issue":"4","key":"2025101705301359000_2025.10.13.682194v1.92","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1016\/j.ymthe.2025.02.041","article-title":"Nasal delivery of secretory IgA confers enhanced neutralizing activity against Omicron variants compared to its IgG counterpart","volume":"33","year":"2025","journal-title":"Mol Ther"},{"issue":"2","key":"2025101705301359000_2025.10.13.682194v1.93","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.vaccine.2011.11.028","article-title":"Oral vaccination with inactivated influenza vaccine induces cross-protective immunity","volume":"30","year":"2012","journal-title":"Vaccine"},{"key":"2025101705301359000_2025.10.13.682194v1.94","doi-asserted-by":"crossref","unstructured":"Li, Z. , et al., Adjuvantation of Influenza Vaccines to Induce Cross-Protective Immunity. Vaccines (Basel), 2021. 9(2).","DOI":"10.3390\/vaccines9020075"},{"key":"2025101705301359000_2025.10.13.682194v1.95","doi-asserted-by":"crossref","unstructured":"Bissett, C. , et al., Systemic prime mucosal boost significantly increases protective efficacy of bivalent RSV influenza viral vectored vaccine. Npj Vaccines, 2024. 9(1).","DOI":"10.1038\/s41541-024-00912-1"},{"issue":"15","key":"2025101705301359000_2025.10.13.682194v1.96","doi-asserted-by":"crossref","first-page":"3505","DOI":"10.1016\/j.vaccine.2024.04.054","article-title":"Cross-protective efficacy and safety of an adenovirus-based universal influenza vaccine expressing nucleoprotein, hemagglutinin, and the ectodomain of matrix protein 2","volume":"42","year":"2024","journal-title":"Vaccine"},{"key":"2025101705301359000_2025.10.13.682194v1.97","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.09.007"},{"issue":"6","key":"2025101705301359000_2025.10.13.682194v1.98","doi-asserted-by":"crossref","first-page":"2127292","DOI":"10.1080\/21645515.2022.2127292","article-title":"A single intranasal administration of AdCOVID protects against SARS-CoV-2 infection in the upper and lower respiratory tracts","volume":"18","year":"2022","journal-title":"Hum Vaccin Immunother"},{"issue":"63","key":"2025101705301359000_2025.10.13.682194v1.99","doi-asserted-by":"crossref","first-page":"eabg6895","DOI":"10.1126\/sciimmunol.abg6895","article-title":"Lung dendritic cells migrate to the spleen to prime long-lived TCF1(hi) memory CD8(+) T cell precursors after influenza infection","volume":"6","year":"2021","journal-title":"Sci Immunol"},{"key":"2025101705301359000_2025.10.13.682194v1.100","doi-asserted-by":"publisher","DOI":"10.2144\/000112729"},{"issue":"7","key":"2025101705301359000_2025.10.13.682194v1.101","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.3201\/eid2707.210462","article-title":"Multiplex Real-Time Reverse Transcription PCR for Influenza A Virus, Influenza B Virus, and Severe Acute Respiratory Syndrome Coronavirus 2","volume":"27","year":"2021","journal-title":"Emerg Infect Dis"},{"key":"2025101705301359000_2025.10.13.682194v1.102","doi-asserted-by":"crossref","unstructured":"King, R.G. , et al., Single-Dose Intranasal Administration of AdCOVID Elicits Systemic and Mucosal Immunity against SARS-CoV-2 and Fully Protects Mice from Lethal Challenge. Vaccines (Basel), 2021. 9(8).","DOI":"10.3390\/vaccines9080881"},{"key":"2025101705301359000_2025.10.13.682194v1.103","doi-asserted-by":"crossref","unstructured":"Curtiss, M.L. , et al., CXCR5 Signals Fine-Tune Dendritic Cell Transcription and Regulate T(H)2 Development. Vaccines (Basel), 2025. 13(9).","DOI":"10.3390\/vaccines13090943"},{"key":"2025101705301359000_2025.10.13.682194v1.104","doi-asserted-by":"publisher","DOI":"10.1110\/ps.8.4.921"},{"key":"2025101705301359000_2025.10.13.682194v1.105","doi-asserted-by":"publisher","DOI":"10.1038\/nature07444"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.10.13.682194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:48:44Z","timestamp":1768470524000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.10.13.682194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,14]]},"references-count":105,"URL":"https:\/\/doi.org\/10.1101\/2025.10.13.682194","relation":{},"subject":[],"published":{"date-parts":[[2025,10,14]]},"subtype":"preprint"}}