{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:48:05Z","timestamp":1789606085831,"version":"build-2803163510"},"posted":{"date-parts":[[2025,10,19]]},"group-title":"Developmental Biology","reference-count":55,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:00:00Z","timestamp":1760832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["R35 NS122191"],"award-info":[{"award-number":["R35 NS122191"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 NS124166"],"award-info":[{"award-number":["R01 NS124166"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]},{"award":["F32 NS134612"],"award-info":[{"award-number":["F32 NS134612"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Nervous system development relies on sequential and coordinated formation of diverse neurons and glia from neural progenitor cells (NPCs). In the spinal cord, NPCs of the pMN domain produce neurons early in development followed by oligodendrocyte precursor cells (OPCs), which subsequently differentiate as oligodendrocytes (OLs), the myelinating glia of the central nervous system, later in development. The mechanisms that specify neural progenitor cells to the OL lineage are not yet well understood. Using zebrafish as an experimental model system, we generated single-cell RNA sequencing and single-nuclei ATAC sequencing data that identified a subpopulation of NPCs, called pre-OPCs, that appeared fated to produce OPCs. pre-OPCs uniquely express several genes that encode transcription factors specific to the OL lineage, including Gsx2, which regulates OPC formation in the mouse forebrain. To investigate Gsx2 function in zebrafish OPC specification, we used CRISPR\/Cas9 genome editing to create\n                  <jats:italic>gsx2<\/jats:italic>\n                  loss-of-function alleles.\n                  <jats:italic>gsx2<\/jats:italic>\n                  homozygous mutant embryos initiated OPC formation prematurely and produced excess OPCs without altering OL differentiation. Using our single-nuclei multi-omics dataset, we predicted a gene regulatory network centered around\n                  <jats:italic>gsx2<\/jats:italic>\n                  and identified genes that might be transcriptionally regulated by Gsx2. Taken together, our studies suggest that Gsx2 expression in pre-OPCs contributes to the timing of OPC specification.\n                <\/jats:p>","DOI":"10.1101\/2025.10.18.683250","type":"posted-content","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T03:55:12Z","timestamp":1760932512000},"source":"Crossref","is-referenced-by-count":1,"title":["Gsx2 Regulates Oligodendrocyte Precursor Formation in the Zebrafish Spinal Cord"],"prefix":"10.64898","author":[{"given":"Kimberly A.","family":"Arena","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Kearns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamoud","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"O\u2019Rourke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles G.","family":"Sagerstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4478-5634","authenticated-orcid":false,"given":"Santos J.","family":"Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4500-0672","authenticated-orcid":false,"given":"Bruce","family":"Appel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025102302152427000_2025.10.18.683250v1.1","doi-asserted-by":"crossref","unstructured":"Buckley, C.E. , Marguerie, A. , Alderton, W.K. , Franklin, R.J ., 2010. Temporal dynamics of myelination in the zebrafish spinal cord, Glia, pp. 802\u2013812.","DOI":"10.1002\/glia.20964"},{"key":"2025102302152427000_2025.10.18.683250v1.2","doi-asserted-by":"crossref","unstructured":"Chapman, H. , Riesenberg, A. , Ehrman, L.A. , Kohli, V. , Nardini, D. , Nakafuku, M. , Campbell, K. , Waclaw, R.R ., 2018. Gsx transcription factors control neuronal versus glial specification in ventricular zone progenitors of the mouse lateral ganglionic eminence, Developmental Biology. Elsevier Inc., pp. 115\u2013126.","DOI":"10.1016\/j.ydbio.2018.07.005"},{"key":"2025102302152427000_2025.10.18.683250v1.3","doi-asserted-by":"crossref","unstructured":"Chapman, H. , Waclaw, R.R. , Pei, Z. , Nakafuku, M. , Campbell, K ., 2012. The homeobox gene Gsx2 controls the timing of oligodendroglial fate specification in mouse lateral ganglionic eminence progenitors, Development (Cambridge), pp. 2289\u20132298.","DOI":"10.1242\/dev.091090"},{"key":"2025102302152427000_2025.10.18.683250v1.4","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.23.5007"},{"key":"2025102302152427000_2025.10.18.683250v1.5","doi-asserted-by":"crossref","unstructured":"Danesin, C. , Agius, E. , Escalas, N. , Ai, X. , Emerson, C. , Cochard, P. , Soula, C ., 2006. Ventral neural progenitors switch toward an oligodendroglial fate in response to increased Sonic hedgehog (Shh) activity: involvement of Sulfatase 1 in modulating Shh signaling in the ventral spinal cord, J Neurosci, pp. 5037\u20135048.","DOI":"10.1523\/JNEUROSCI.0715-06.2006"},{"key":"2025102302152427000_2025.10.18.683250v1.6","doi-asserted-by":"crossref","unstructured":"Danesin, C. , Soula, C ., 2017. Moving the Shh Source over Time: What Impact on Neural Cell Diversification in the Developing Spinal Cord?, Journal of Developmental Biology, p. 4.","DOI":"10.3390\/jdb5020004"},{"key":"2025102302152427000_2025.10.18.683250v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.11.019"},{"key":"2025102302152427000_2025.10.18.683250v1.8","doi-asserted-by":"crossref","unstructured":"Dessaud, E. , McMahon, A.P. , Briscoe, J ., 2008. Pattern formation in the vertebrate neural tube: a sonic hedgehog morphogen-regulated transcriptional network., Development (Cambridge, England), pp. 2489\u20132503.","DOI":"10.1242\/dev.009324"},{"key":"2025102302152427000_2025.10.18.683250v1.9","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041358"},{"key":"2025102302152427000_2025.10.18.683250v1.10","doi-asserted-by":"crossref","unstructured":"Farreny, M.-A. , Agius, E. , Bel-Vialar, S. , Escalas, N. , Khouri-Farah, N. , Soukkarieh, C. , Danesin, C. , Pituello, F. , Cochard, P. , Soula, C ., 2018. FGF signaling controls Shh-dependent oligodendroglial fate specification in the ventral spinal cord, Neural Development. Neural Development, p. 3.","DOI":"10.1101\/233775"},{"key":"2025102302152427000_2025.10.18.683250v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05279-8"},{"key":"2025102302152427000_2025.10.18.683250v1.12","doi-asserted-by":"crossref","first-page":"108788","DOI":"10.1016\/j.celrep.2021.108788","article-title":"Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex","volume":"34","year":"2021","journal-title":"Cell Reports"},{"key":"2025102302152427000_2025.10.18.683250v1.13","doi-asserted-by":"crossref","unstructured":"Gao, Y. , Sun, M. , Fu, T. , Wang, Z. , Jiang, X. , Yang, L. , Liang, X.G. , Liu, G. , Tian, Y. , Yang, F. , Li, J. , Li, Z. , Li, X. , You, Y. , Ding, C. , Wang, Y. , Ma, T. , Zhang, Z. , Xu, Z. , Chen, B. , Yang, Z ., 2025. NOTCH, ERK, and SHH signaling respectively control the fate determination of cortical glia and olfactory bulb interneurons. Proceedings of the National Academy of Sciences 122.","DOI":"10.1073\/pnas.2416757122"},{"key":"2025102302152427000_2025.10.18.683250v1.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4787-14.2015"},{"key":"2025102302152427000_2025.10.18.683250v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020s.06.027"},{"key":"2025102302152427000_2025.10.18.683250v1.16","doi-asserted-by":"crossref","unstructured":"Jiang, W. , Ishino, Y. , Hashimoto, H. , Keino-Masu, K. , Masu, M. , Uchimura, K. , Kadomatsu, K. , Yoshimura, T. , Ikenaka, K ., 2017. Sulfatase 2 Modulates Fate Change from Motor Neurons to Oligodendrocyte Precursor Cells through Coordinated Regulation of Shh Signaling with Sulfatase 1, Developmental Neuroscience, pp. 361\u2013374.","DOI":"10.1159\/000464284"},{"key":"2025102302152427000_2025.10.18.683250v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/nn1620"},{"key":"2025102302152427000_2025.10.18.683250v1.18","doi-asserted-by":"crossref","unstructured":"Kimmel, C.B. , Ballard, W.W. , Kimmel, S.R. , Ullmann, B. , Schilling, T.F ., 1995. Stages of embryonic development of the zebrafish, Dev Dyn, pp. 253\u2013310.","DOI":"10.1002\/aja.1002030302"},{"key":"2025102302152427000_2025.10.18.683250v1.19","doi-asserted-by":"crossref","unstructured":"Lu, Q.R. , Sun, T. , Zhu, Z. , Ma, N. , Garcia, M. , Stiles, C.D. , Rowitch, D.H ., 2002. Common Developmental Requirement for Olig Function Indicates a Motor Neuron \/ Oligodendrocyte Connection, pp. 75\u201386.","DOI":"10.1016\/S0092-8674(02)00678-5"},{"key":"2025102302152427000_2025.10.18.683250v1.20","doi-asserted-by":"crossref","unstructured":"Lu, Q.R. , Yuk, D. , Alberta, J.A. , Zhu, Z. , Pawlitzky, I. , Chan, J. , McMahon, A.P. , Stiles, C.D. , Rowitch, D.H ., 2000. Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system, Neuron, pp. 317\u2013329.","DOI":"10.1016\/S0896-6273(00)80897-1"},{"key":"2025102302152427000_2025.10.18.683250v1.21","doi-asserted-by":"crossref","unstructured":"Marques, S. , van Bruggen, D. , Vanichkina, D.P. , Floriddia, E.M. , Munguba, H. , V\u00e4remo, L. , Giacomello, S. , Falc\u00e3o, A.M. , Meijer, M. , Bj\u00f6rklund, \u00c5.K. , Hjerling-Leffler, J. , Taft, R.J. , Castelo-Branco, G. , 2018. Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development, Developmental Cell, pp. 504\u2013517.e507.","DOI":"10.1016\/j.devcel.2018.07.005"},{"key":"2025102302152427000_2025.10.18.683250v1.22","doi-asserted-by":"crossref","unstructured":"Masahira, N. , Takebayashi, H. , Ono, K. , Watanabe, K. , Ding, L. , Furusho, M. , Ogawa, Y. , Nabeshima, Y. , Alvarez-Buylla, A. , Shimizu, K. , Ikenaka, K ., 2006. Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells, Dev Biol, pp. 358\u2013369.","DOI":"10.1016\/j.ydbio.2006.02.029"},{"key":"2025102302152427000_2025.10.18.683250v1.23","doi-asserted-by":"crossref","unstructured":"Myers, P.Z. , Eisen, J.S. , Westerfield, M ., 1986. Development and axonal outgrowth of identified motoneurons in the zebrafish, J Neurosci, pp. 2278\u20132289.","DOI":"10.1523\/JNEUROSCI.06-08-02278.1986"},{"key":"2025102302152427000_2025.10.18.683250v1.24","doi-asserted-by":"crossref","unstructured":"Oustah, A.A. , Danesin, C. , Khouri-Farah, N. , Farreny, M.-A. , Escalas, N. , Cochard, P. , Glise, B. , Soula, C ., 2014. Dynamics of Sonic hedgehog signaling in the ventral spinal cord are controlled by intrinsic changes in source cells requiring Sulfatase 1., Development (Cambridge, England), pp. 1392\u20131403.","DOI":"10.1242\/dev.101717"},{"key":"2025102302152427000_2025.10.18.683250v1.25","doi-asserted-by":"crossref","unstructured":"Park, H. , Mehta, A. , Richardson, J.S. , Appel, B ., 2002. olig2 is required for zebrafish primary motor neuron and oligodendrocyte development, Developmental Biology, pp. 356\u2013368.","DOI":"10.1006\/dbio.2002.0738"},{"key":"2025102302152427000_2025.10.18.683250v1.26","doi-asserted-by":"crossref","unstructured":"Park, H. , Shin, J. , Appel, B ., 2004. Spatial and temporal regulation of ventral spinal cord precursor specification by Hedgehog signaling, Development, pp. 5959\u20135969.","DOI":"10.1242\/dev.01456"},{"key":"2025102302152427000_2025.10.18.683250v1.27","doi-asserted-by":"crossref","unstructured":"Park, H.-C.C. , Boyce, J. , Shin, J. , Appel, B ., 2005. Oligodendrocyte specification in zebrafish requires notch-regulated cyclin-dependent kinase inhibitor function, J Neurosci, pp. 6836\u20136844.","DOI":"10.1523\/JNEUROSCI.0981-05.2005"},{"key":"2025102302152427000_2025.10.18.683250v1.28","doi-asserted-by":"crossref","unstructured":"Parras, C.M. , Hunt, C. , Sugimori, M. , Nakafuku, M. , Rowitch, D. , Guillemot, F ., 2007. The Proneural Gene Mash1 Specifies an Early Population of Telencephalic Oligodendrocytes, Journal of Neuroscience, pp. 4233\u20134242.","DOI":"10.1523\/JNEUROSCI.0126-07.2007"},{"key":"2025102302152427000_2025.10.18.683250v1.29","doi-asserted-by":"crossref","unstructured":"Ravanelli, A.M. , Appel, B ., 2015. Motor neurons and oligodendrocytes arise from distinct cell lineages by progenitor recruitment, Genes & Development, pp. 2504\u20132515.","DOI":"10.1101\/gad.271312.115"},{"key":"2025102302152427000_2025.10.18.683250v1.30","doi-asserted-by":"crossref","unstructured":"Rowitch, D.H ., 2004. Glial specification in the vertebrate neural tube., Nature reviews. Neuroscience, pp. 409\u2013 419.","DOI":"10.1038\/nrn1389"},{"key":"2025102302152427000_2025.10.18.683250v1.31","doi-asserted-by":"crossref","unstructured":"Roychoudhury, K. , Salomone, J. , Qin, S. , Cain, B. , Adam, M. , Steven Potter, S. , Nakafuku, M. , Gebelein, B. , Campbell, K ., 2020. Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence, Development (Cambridge).","DOI":"10.1101\/794511"},{"key":"2025102302152427000_2025.10.18.683250v1.32","doi-asserted-by":"crossref","unstructured":"Scott, K. , O\u2019Rourke, R. , Gillen, A. , Appel, B ., 2020. Prdm8 regulates pMN progenitor specification for motor neuron and oligodendrocyte fates by modulating Shh signaling response., Development (Cambridge, England). Development.","DOI":"10.1242\/dev.191023"},{"key":"2025102302152427000_2025.10.18.683250v1.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2021.07.010"},{"key":"2025102302152427000_2025.10.18.683250v1.34","doi-asserted-by":"crossref","unstructured":"Shin, J. , Park, H.C. , Topczewska, J.M. , Mawdsley, D.J. , Appel, B ., 2003. Neural cell fate analysis in zebrafish using olig2 BAC transgenics, Methods Cell Sci, pp. 7\u201314.","DOI":"10.1023\/B:MICS.0000006847.09037.3a"},{"key":"2025102302152427000_2025.10.18.683250v1.35","doi-asserted-by":"crossref","unstructured":"Stolt, C.C. , Schlierf, A. , Lommes, P. , Hillgartner, S. , Werner, T. , Kosian, T. , Sock, E. , Kessaris, N. , Richardson, W.D. , Lefebvre, V. , Wegner, M ., 2006. SoxD proteins influence multiple stages of oligodendrocyte development and modulate SoxE protein function, Dev Cell, pp. 697\u2013709.","DOI":"10.1016\/j.devcel.2006.08.011"},{"key":"2025102302152427000_2025.10.18.683250v1.36","doi-asserted-by":"crossref","unstructured":"Street, K. , Risso, D. , Fletcher, R.B. , Das, D. , Ngai, J. , Yosef, N. , Purdom, E. , Dudoit, S ., 2018. Slingshot: cell lineage and pseudotime inference for single-cell transcriptomics., BMC genomics. BMC Genomics, p. 477.","DOI":"10.1101\/128843"},{"key":"2025102302152427000_2025.10.18.683250v1.37","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1038\/s41592-021-01282-5","article-title":"Single-cell chromatin state analysis with Signac","volume":"18","year":"2021","journal-title":"Nature Methods"},{"key":"2025102302152427000_2025.10.18.683250v1.38","doi-asserted-by":"crossref","unstructured":"Sugimori, M. , Nagao, M. , Bertrand, N. , Parras, C.M. , Guillemot, F. , Nakafuku, M ., 2007. Combinatorial actions of patterning and HLH transcription factors in the spatiotemporal control of neurogenesis and gliogenesis in the developing spinal cord, Development, pp. 1617\u20131629.","DOI":"10.1242\/dev.001255"},{"key":"2025102302152427000_2025.10.18.683250v1.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.11.001"},{"key":"2025102302152427000_2025.10.18.683250v1.40","first-page":"143","article-title":"Dynamic expression of basic helix-loop-helix Olig family members: implication of Olig2 in neuron and oligodendrocyte differentiation and identification of a new member","volume":"3","year":"2000","journal-title":"Olig"},{"key":"2025102302152427000_2025.10.18.683250v1.41","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.20.4361"},{"key":"2025102302152427000_2025.10.18.683250v1.42","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0144-23.2023"},{"key":"2025102302152427000_2025.10.18.683250v1.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.04.016"},{"key":"2025102302152427000_2025.10.18.683250v1.44","doi-asserted-by":"crossref","unstructured":"Vue, T.Y. , Kim, E.J. , Parras, C.M. , Guillemot, F. , Johnson, J.E ., 2014. Ascl1 controls the number and distribution of astrocytes and oligodendrocytes in the gray matter and white matter of the spinal cord, Development, pp. 3721\u20133731.","DOI":"10.1242\/dev.105270"},{"key":"2025102302152427000_2025.10.18.683250v1.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.07.015"},{"key":"2025102302152427000_2025.10.18.683250v1.46","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.3892\/mmr.2017.6843","article-title":"Transcription factor 7 like 2 promotes oligodendrocyte differentiation and remyelination","volume":"16","year":"2017","journal-title":"Molecular Medicine Reports"},{"key":"2025102302152427000_2025.10.18.683250v1.47","doi-asserted-by":"crossref","unstructured":"Weng, Q. , Wang, J. , Wang, J. , He, D. , Cheng, Z. , Zhang, F. , Verma, R. , Xu, L. , Dong, X. , Liao, Y. , He, X. , Potter, A. , Zhang, L. , Zhao, C. , Xin, M. , Zhou, Q. , Aronow, B.J. , Blackshear, P.J. , Rich, J.N. , He, Q. , Zhou, W. , Suv\u00e0, M.L. , Waclaw, R.R. , Potter, S.S. , Yu, G. , Lu, Q.R ., 2019. Single-Cell Transcriptomics Uncovers Glial Progenitor Diversity and Cell Fate Determinants during Development and Gliomagenesis, Cell Stem Cell, pp. 1\u201317.","DOI":"10.1016\/j.stem.2019.03.006"},{"key":"2025102302152427000_2025.10.18.683250v1.48","doi-asserted-by":"crossref","unstructured":"Winkler, C.C. , Tran, L.N. , Milan, E.P. , Garc\u00eda-Moreno, F. , Franco, S.J. , 2020. Unraveling the transcriptional networks that drive oligodendrocyte fate specification in Sonic hedgehog-responsive neocortical progenitors, bioRxiv. Cold Spring Harbor Laboratory, p. 2020.2010.2018.344515.","DOI":"10.1101\/2020.10.18.344515"},{"key":"2025102302152427000_2025.10.18.683250v1.49","doi-asserted-by":"crossref","unstructured":"Zaucker, A. , Mercurio, S. , Sternheim, N. , Talbot, W.S. , Marlow, F.L ., 2013. Notch3 Is Essential for Oligodendrocyte Development and Vascular Integrity in Zebrafish., Disease models & mechanisms, pp. 1246\u2013 1259.","DOI":"10.1242\/dmm.012005"},{"key":"2025102302152427000_2025.10.18.683250v1.50","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2386-20.2021"},{"key":"2025102302152427000_2025.10.18.683250v1.51","doi-asserted-by":"crossref","unstructured":"Zhang, Y. , Liu, G. , Guo, T. , Liang, X.G. , Du, H. , Yang, L. , Bhaduri, A. , Li, X. , Xu, Z. , Zhang, Z. , Li, Z. , He, M. , Tsyporin, J. , Kriegstein, A.R. , Rubenstein, J.L. , Yang, Z. , Chen, B ., 2020. Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog, Cell Reports. ElsevierCompany., pp. 4490\u20134504.e4494.","DOI":"10.1016\/j.celrep.2020.03.027"},{"key":"2025102302152427000_2025.10.18.683250v1.52","doi-asserted-by":"crossref","first-page":"10883","DOI":"10.1038\/ncomms10883","article-title":"Dual regulatory switch through interactions of Tcf7l2\/Tcf4 with stage-specific partners propels oligodendroglial maturation","volume":"7","year":"2016","journal-title":"Nature Communications"},{"key":"2025102302152427000_2025.10.18.683250v1.53","doi-asserted-by":"crossref","unstructured":"Zhou, Q. , Anderson, D.J ., 2002. The bHLH transcription factors OLIG2 and OLIG1 couple neuronal and glial subtype specification, Cell, pp. 61\u201373.","DOI":"10.1016\/S0092-8674(02)00677-3"},{"key":"2025102302152427000_2025.10.18.683250v1.54","doi-asserted-by":"crossref","unstructured":"Zhou, Q. , Wang, S. , Anderson, D.J ., 2000. Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors, Neuron, pp. 331\u2013343.","DOI":"10.1016\/S0896-6273(00)80898-3"},{"key":"2025102302152427000_2025.10.18.683250v1.55","doi-asserted-by":"publisher","DOI":"10.1242\/dev.095323"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.10.18.683250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:04:41Z","timestamp":1782277481000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.10.18.683250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,19]]},"references-count":55,"URL":"https:\/\/doi.org\/10.1101\/2025.10.18.683250","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1016\/j.ydbio.2026.01.001","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2025,10,19]]},"subtype":"preprint"}}