{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:18:21Z","timestamp":1785421101678,"version":"3.56.0"},"posted":{"date-parts":[[2025,10,21]]},"group-title":"Immunology","reference-count":40,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["R01 HL138931"],"award-info":[{"award-number":["R01 HL138931"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["RO1 HL153341"],"award-info":[{"award-number":["RO1 HL153341"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["T31 HL160491"],"award-info":[{"award-number":["T31 HL160491"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Abdominal aortic aneurysms (AAAs) are chronic inflammatory vascular disorders characterized by progressive aortic dilation and destruction of the vascular wall, often culminating in rupture. Current management is limited to surgical repair, with no approved targeted pharmacologic therapies. In this study, we investigated the immunomodulatory role of Protectin D1 (PD1), a specialized pro-resolving lipid mediator, through G-protein\u2013coupled receptor 37 (GPR37) signaling on macrophages in mitigating AAA progression and preventing aortic rupture. Single cell-RNA sequencing analysis of human tissue demonstrated significant differences in PD1\/GPR37 axis-related genes in macrophages in AAAs compared to control aortic tissue. Using an established murine AAA model, PD1 administration significantly attenuated aortic diameter, pro-inflammatory cytokine and matrix metalloproteinase (MMP2) expression, as well as maintained aortic morphology in a GPR37-dependent manner. Importantly, PD1 treatment prevented preformed AAA progression to aortic rupture in another preclinical elastase+BAPN model of aortic rupture, by attenuating aortic diameter, tissue inflammation as well as decreasing macrophage infiltration, preserving elastin integrity, and restoring smooth muscle \u03b1-actin expression in the aortic wall. Mechanistically, PD1 enhanced macrophage efferocytosis of apoptotic vascular smooth muscle cells in the murine aortic tissue as well as in isolated macrophages via GPR37-dependent manner and attenuated the inflammatory paracrine secretion of macrophage-specific paracrine release of TNF-\u03b1 and IL-\u03b2. These findings suggest that PD1\/GPR37 signaling on macrophages promotes inflammation-resolution by enhancing efferocytosis of apoptotic SMCs conferring protection against aortic inflammation and remodeling to mitigate AAA formation and rupture.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Significance Statement<\/jats:title>\n                  <jats:p>This study elucidates the protective role of specialized proresolving lipid mediator, Protectin D1, by activating macrophages via GPR37 receptors, to enhance the clearance of apoptotic smooth muscle cells to mitigate aortic inflammation and vascular remodeling during abdominal aortic aneurysm formation. We observed that several key inflammation related genes were associated with PD1\/GPR37-dependent signaling in macrophages of human AAAs. Detailed analysis in experimental models delineated the signaling pathway where upregulating macrophage-dependent efferocytosis via immunomodulation by Protectin D1 attenuated aortic inflammation and remodeling, indicating a potential mechanism for therapeutic intervention in the pathobiology of AAAs to prevent aortic rupture.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.10.20.683538","type":"posted-content","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T22:45:12Z","timestamp":1761086712000},"source":"Crossref","is-referenced-by-count":1,"title":["Protectin D1\/GPR37 signaling enhances macrophage-dependent efferocytosis to attenuate experimental abdominal aortic aneurysm formation"],"prefix":"10.64898","author":[{"given":"Aravinthan","family":"Adithan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Fassler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanyi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff Arni C.","family":"Valisno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiven","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walker","family":"Ueland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashish K.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Gilbert R.","family":"Upchurch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"1","key":"2025102313450752000_2025.10.20.683538v1.1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/s41572-018-0030-7","article-title":"Abdominal aortic aneurysms","volume":"4","year":"2018","journal-title":"Nat Rev Dis Primers"},{"key":"2025102313450752000_2025.10.20.683538v1.2","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2014-305648"},{"key":"2025102313450752000_2025.10.20.683538v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-023-01130-w"},{"key":"2025102313450752000_2025.10.20.683538v1.4","doi-asserted-by":"crossref","first-page":"989933","DOI":"10.3389\/fimmu.2022.989933","article-title":"Immune and inflammatory mechanisms of abdominal aortic aneurysm","volume":"13","year":"2022","journal-title":"Front Immunol"},{"key":"2025102313450752000_2025.10.20.683538v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2017.52"},{"key":"2025102313450752000_2025.10.20.683538v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0240-6"},{"key":"2025102313450752000_2025.10.20.683538v1.7","first-page":"86","article-title":"Mechanisms and Consequences of Defective Efferocytosis in Atherosclerosis","volume":"4","year":"2017","journal-title":"Front Cardiovasc Med"},{"key":"2025102313450752000_2025.10.20.683538v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.08.002"},{"key":"2025102313450752000_2025.10.20.683538v1.9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99888"},{"issue":"1","key":"2025102313450752000_2025.10.20.683538v1.10","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1038\/s41467-021-21940-8","article-title":"Activation of GPR37 in macrophages confers protection against infection-induced sepsis and pain-like behaviour in mice","volume":"12","year":"2021","journal-title":"Nat Commun"},{"key":"2025102313450752000_2025.10.20.683538v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/nature11042"},{"key":"2025102313450752000_2025.10.20.683538v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.10.006"},{"key":"2025102313450752000_2025.10.20.683538v1.13","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20210518"},{"key":"2025102313450752000_2025.10.20.683538v1.14","doi-asserted-by":"crossref","unstructured":"Franzen O , Gan LM , & Bjorkegren JLM (2019) PanglaoDB: a web server for exploration of mouse and human single-cell RNA sequencing data. 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