{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T17:50:23Z","timestamp":1774979423569,"version":"3.50.1"},"posted":{"date-parts":[[2025,10,23]]},"group-title":"Developmental Biology","reference-count":25,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"Fondation Marcel & Rolande Gosselin, NSERC","award":["RGPIN-2019-06863"],"award-info":[{"award-number":["RGPIN-2019-06863"]}]},{"name":"Fondation Marcel & Rolande Gosselin, NSERC","award":["RGPAS-2019-00017"],"award-info":[{"award-number":["RGPAS-2019-00017"]}]},{"name":"Fondation Marcel & Rolande Gosselin, NSERC","award":["DGECR-2019-00326"],"award-info":[{"award-number":["DGECR-2019-00326"]}]},{"DOI":"10.13039\/501100000024","name":"CIHR","doi-asserted-by":"crossref","award":["PJT-169138"],"award-info":[{"award-number":["PJT-169138"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  In\n                  <jats:italic>C. elegans hermaphrodites<\/jats:italic>\n                  , the distal tip cells (DTCs) capping the distal gonad arms provide the niche signal, a Notch ligand, to maintain germline stem cell pools. Using fixed germlines, it was recently shown that the transcription of a Notch target gene decreased relatively early-on during adulthood. Here, we used the genetically encoded Notch Sensor Able to detect Lateral Signaling Activity (SALSA) to examine the pattern of GLP-1\/Notch activity across the aging distal gonad\n                  <jats:italic>in vivo<\/jats:italic>\n                  . Interestingly, we find that the robust and progressively decreasing distal-proximal Notch activation gradient that is observed in young adults gets entirely lost during aging.\n                <\/jats:p>","DOI":"10.1101\/2025.10.22.683991","type":"posted-content","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T22:50:14Z","timestamp":1761259814000},"source":"Crossref","is-referenced-by-count":0,"title":["Loss of the distal-proximal GLP-1\/Notch activation gradient in the aging C. elegans germline"],"prefix":"10.64898","author":[{"given":"Rustelle Janse","family":"van Vuuren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pier-Olivier","family":"Martel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Narbonne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025102703000568000_2025.10.22.683991v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90128-0"},{"key":"2025102703000568000_2025.10.22.683991v1.2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.mad.2015.09.006","article-title":"Cytoplasmic mRNA turnover and ageing","volume":"152","year":"2015","journal-title":"Mechanisms of ageing and development"},{"key":"2025102703000568000_2025.10.22.683991v1.3","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/20031967725"},{"key":"2025102703000568000_2025.10.22.683991v1.4","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-03-0170"},{"key":"2025102703000568000_2025.10.22.683991v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05922-y"},{"key":"2025102703000568000_2025.10.22.683991v1.6","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01034-07"},{"key":"2025102703000568000_2025.10.22.683991v1.7","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.9.1751"},{"key":"2025102703000568000_2025.10.22.683991v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nature01135"},{"key":"2025102703000568000_2025.10.22.683991v1.9","doi-asserted-by":"crossref","first-page":"e25.","DOI":"10.1371\/journal.pgen.0030025","article-title":"Genetic and pharmacological factors that influence reproductive aging in nematodes","volume":"3","year":"2007","journal-title":"PLoS genetics"},{"key":"2025102703000568000_2025.10.22.683991v1.10","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.130450"},{"key":"2025102703000568000_2025.10.22.683991v1.11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1401861111"},{"key":"2025102703000568000_2025.10.22.683991v1.12","first-page":"173195","article-title":"Rapid population-wide declines in stem cell number and activity duringreproductive aging in C. elegans","volume":"146","year":"2019","journal-title":"Development"},{"key":"2025102703000568000_2025.10.22.683991v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.03.045"},{"key":"2025102703000568000_2025.10.22.683991v1.14","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03781-z"},{"key":"2025102703000568000_2025.10.22.683991v1.15","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.18370"},{"key":"2025102703000568000_2025.10.22.683991v1.16","doi-asserted-by":"crossref","first-page":"e1000789.","DOI":"10.1371\/journal.pgen.1000789","article-title":"TGF-\u00df Sma\/Mab signaling mutations uncouple reproductive aging from somatic aging","volume":"5","year":"2009","journal-title":"PLoS genetics"},{"key":"2025102703000568000_2025.10.22.683991v1.17","unstructured":"Martel PO , Janse van Vuuren R , Degr\u00e9mont J , Turmel-Couture S , Chaudhari AM , Dologuele E , Beaulieu L , Clouet A. Narbonne P. Niche-associated Type IV collagen promotes GLP-1\/Notch receptor activation in the C. elegans germline. Nat. Commun. In press."},{"key":"2025102703000568000_2025.10.22.683991v1.18","doi-asserted-by":"publisher","DOI":"10.1242\/dev.042523"},{"key":"2025102703000568000_2025.10.22.683991v1.19","doi-asserted-by":"publisher","DOI":"10.1242\/dev.130252"},{"key":"2025102703000568000_2025.10.22.683991v1.20","doi-asserted-by":"crossref","first-page":"e1006738.","DOI":"10.1371\/journal.pgen.1006738","article-title":"DAF-18\/PTEN signals through AAK-1\/AMPK to inhibit MPK- 1\/MAPK in feedback control of germline stem cell proliferation","volume":"13","year":"2017","journal-title":"PLoS genetics"},{"key":"2025102703000568000_2025.10.22.683991v1.21","first-page":"19","article-title":"Non-autonomous regulation of germline stem cell proliferation by somatic MPK-1\/MAPK activity in C. elegans","volume":"35","year":"2021","journal-title":"Cell reports"},{"key":"2025102703000568000_2025.10.22.683991v1.22","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1146"},{"key":"2025102703000568000_2025.10.22.683991v1.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.03.008"},{"key":"2025102703000568000_2025.10.22.683991v1.24","doi-asserted-by":"crossref","first-page":"bio060261.","DOI":"10.1242\/bio.060261","article-title":"Aging disrupts spatiotemporal regulation of germline stem cells and niche integrity","volume":"13","year":"2024","journal-title":"Biology Open"},{"key":"2025102703000568000_2025.10.22.683991v1.25","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-11-0716"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.10.22.683991","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:52:37Z","timestamp":1768470757000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.10.22.683991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,23]]},"references-count":25,"URL":"https:\/\/doi.org\/10.1101\/2025.10.22.683991","relation":{},"subject":[],"published":{"date-parts":[[2025,10,23]]},"subtype":"preprint"}}