{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T01:58:26Z","timestamp":1768528706493,"version":"3.49.0"},"posted":{"date-parts":[[2025,10,25]]},"group-title":"Bioengineering","reference-count":44,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"crossref","award":["R01EB029418"],"award-info":[{"award-number":["R01EB029418"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,25]]},"abstract":"<jats:p>\n                  Fibrotic encapsulation of implanted biomedical devices represents the highest cause of device failure across numerous fields, including orthopedics, breast implants and neural electrodes. While most research has focused on the immune system for driving the foreign body response to devices, fibroblasts are emerging as another key regulator of tissue response. In the present study, we evaluated the effects of porous device design on the activation of primary human dermal fibroblasts into myofibroblasts, a contractile cell that is a hallmark of fibrosis, over four weeks in culture. In particular two factors were studied 1) the introduction of nanoparticles, as they are increasingly being used for device functionalization, and 2) the polymer matrix, which dictates chemistry, mechanics and degradation. The addition of tantalum oxide (TaOx) nanoparticles (0-20wt%) had a minimal effect on fibroblasts, significantly down-regulating expression of alpha smooth muscle actin (\u03b1SMA) (0.31 \u00b1 0.1), likely driven by the increase in nanoscale surface roughness. The polymer matrix of the device caused significant changes to myofibroblast activation, with the fast degrading polymer, poly(lactide co-glycolide) (PLGA) 50:50, significantly up-regulating multiple myofibroblast markers, including \u03b1SMA (5.16 \u00b1 2.5), vinculin, integrin \u03b2\n                  <jats:sub>1<\/jats:sub>\n                  , and integrin \u03b2\n                  <jats:sub>5<\/jats:sub>\n                  , compared to non-degrading polycaprolactone (PCL). The effect was due to the release of degradation products, namely lactic acid, which affects cellular metabolism. Together this highlights that device design affects biological response immediately post-implantation in ways that impact the ultimate success or failure of biomedical devices.\n                <\/jats:p>","DOI":"10.1101\/2025.10.24.684439","type":"posted-content","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T10:35:13Z","timestamp":1761388513000},"source":"Crossref","is-referenced-by-count":0,"title":["Distinct Roles of Surface Nanostructure and Polymer Degradation in Fibroblast Response to Device Design"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2606-4136","authenticated-orcid":false,"given":"Kendell M","family":"Pawelec","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0901-5781","authenticated-orcid":false,"given":"Erik M.","family":"Shapiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025102804201028000_2025.10.24.684439v1.1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2023.0300"},{"key":"2025102804201028000_2025.10.24.684439v1.2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202304478"},{"key":"2025102804201028000_2025.10.24.684439v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-024-00716-0"},{"issue":"2","key":"2025102804201028000_2025.10.24.684439v1.4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1089\/wound.2022.0066","article-title":"Exploring the overlooked roles and mechanisms of fibroblasts in the foreign body response","volume":"12","year":"2023","journal-title":"Adv. wound care"},{"key":"2025102804201028000_2025.10.24.684439v1.5","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/ncprheum0428","article-title":"Mechanisms of disease: molecular insights into aseptic loosening of orthopedic implants","volume":"3","year":"2007","journal-title":"Nat. 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