{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T02:32:20Z","timestamp":1768530740672,"version":"3.49.0"},"posted":{"date-parts":[[2025,10,28]]},"group-title":"Immunology","reference-count":33,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The growing food allergy epidemic is thought to be related to changing environmental factors, particularly changes in the gut microbiome. While prior work has demonstrated that food allergy can be modulated by gut microbes, little is known about how food allergen-specific CD4\n                  <jats:sup>+<\/jats:sup>\n                  T cells are affected by gut microbial composition. Here, we report that food allergy severity differs between mice obtained from two different specific pathogen-free mouse vendors (Jackson Labs [Jax] and Taconic Biosciences [Tac]). Mice from Tac develop diarrhea and anaphylaxis after fewer allergen exposures than mice from Jax. Using food allergen peptide:MHCII tetramers, we also find that Tac mice have fewer allergen-specific regulatory T cells in the small intestine compared to mice from Jax. In addition, Tac mice have a greater abundance of small intestinal mucosal mast cells and increased intestinal permeability. The increased food allergy severity phenotype is transferable via co-housing, which corresponds to a shift in Jax microbial communities towards those found in Tac mice. Our findings demonstrate for the first time that food allergen-specific Treg cells can be modulated by gut microbial community composition, which in turn is correlated to food allergy severity.\n                <\/jats:p>","DOI":"10.1101\/2025.10.27.684919","type":"posted-content","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T21:00:12Z","timestamp":1761685212000},"source":"Crossref","is-referenced-by-count":0,"title":["Gut microbial composition modulates food-specific CD4\n                  <sup>+<\/sup>\n                  T cells in food allergy"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1567-1320","authenticated-orcid":false,"given":"Alexa R","family":"Weingarden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flannery S","family":"Dahlberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline N","family":"Broude","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiandong","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5888-3702","authenticated-orcid":false,"given":"Sunit","family":"Jain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6694-6964","authenticated-orcid":false,"given":"Allison M","family":"Weakley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley V","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2810-6996","authenticated-orcid":false,"given":"Thamotharampillai","family":"Dileepan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3079-8247","authenticated-orcid":false,"given":"Michael A","family":"Fischbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8009-7655","authenticated-orcid":false,"given":"Marc K","family":"Jenkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025103020000851000_2025.10.27.684919v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.03.048"},{"key":"2025103020000851000_2025.10.27.684919v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.10.019"},{"key":"2025103020000851000_2025.10.27.684919v1.3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1412008111"},{"key":"2025103020000851000_2025.10.27.684919v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0461-z"},{"key":"2025103020000851000_2025.10.27.684919v1.5","doi-asserted-by":"crossref","unstructured":"Schwarzer M , Schabussova I. 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