{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:50:30Z","timestamp":1785412230186,"version":"3.56.0"},"posted":{"date-parts":[[2025,10,31]]},"group-title":"Cancer Biology","reference-count":42,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["R01HL170115"],"award-info":[{"award-number":["R01HL170115"]}],"id":[{"id":"https:\/\/ror.org\/012pb6c26","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AACR MPM oncology charitable foundation transformative cancer research award"],"award-info":[{"award-number":["AACR MPM oncology charitable foundation transformative cancer research award"]}],"id":[{"id":"https:\/\/ror.org\/02fnpv153","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Polycomb targeted loci form long-range chromatin interactions independent of CTCF-cohesion and are demarcated by low DNA methylation(1\u20133). Polycomb targets form extremely long-range loops (long Polycomb loops) that can separate anchors up to 60 Mb. These loops predominantly occur in cells of self-renewal status, such as human hematopoietic stem cells (HSC) and mouse embryonic stem cells (ESC), but rarely in cell lines.<\/jats:p>\n                <jats:p>To identify long Polycomb loops in cancer, we initiated a pan-cancer survey of long Polycomb loops in a collection of 264 tumor samples (33 acute myeloid leukemias, 17 T cell lymphoblastic leukemia, 29 Breast cancer samples, 63 pediatric brain tumors, 70 prostate cancers, and 42 colon cancers). We found most cancers, including all prostate cancers and colon cancers, lack long Polycomb loops except pediatric brain tumors and certain AMLs.<\/jats:p>\n                <jats:p>\n                  In pediatric brain tumors, we found 30% of the ependymoma PFA subtype (which shows globally depleted H3K27me3) notably displayed strong long Polycomb loop interactions. AML displayed more diverse levels of long Polycomb loop interactions. Most AMLs lost both long Polycomb loops with Polycomb binding loss at the loop anchors. Whereas 10% of AMLs retain long Polycomb loops as strong as in HSCs. These AMLs recurrently carry mutations in\n                  <jats:italic>CEBPA<\/jats:italic>\n                  and\n                  <jats:italic>STAG2<\/jats:italic>\n                  , which are not associated with the Polycomb complex or DNA methylation machinery. We found that long Polycomb loop strong AML is sensitive to EZH2 inhibition, which induces cell differentiation. Conversely, PRC1 component dependency can be used to predict long Polycomb loop formation in B-cell lymphoblastic leukemia cell lines.\n                <\/jats:p>\n                <jats:p>Our analysis suggests that the oncogenesis process antagonizes the long Polycomb loop maintenance in most cancers, yet certain cancers may still preserve strong long Polycomb loops from cell-of-origin. The maintenance of long Polycomb loops sensitizes cells to Polycomb inhibition, indicating that such loops could be an epigenomic biomarker for pharmacological or genetic Polycomb inhibition.<\/jats:p>","DOI":"10.1101\/2025.10.30.685561","type":"posted-content","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T20:45:11Z","timestamp":1761943511000},"source":"Crossref","is-referenced-by-count":0,"title":["Pan-cancer 3D genomic analysis revealed extremely long Polycomb loops as the biomarker for sensitivity to Polycomb inhibition"],"prefix":"10.64898","author":[{"given":"Sean","family":"Moran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Zanovello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4951-5788","authenticated-orcid":false,"given":"David Xue Qing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0982-8122","authenticated-orcid":false,"given":"Chao","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9533-4761","authenticated-orcid":false,"given":"Xiaotian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025120408000780000_2025.10.30.685561v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2020.04.018"},{"key":"2025120408000780000_2025.10.30.685561v2.2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2836"},{"key":"2025120408000780000_2025.10.30.685561v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.043"},{"key":"2025120408000780000_2025.10.30.685561v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.01.010"},{"key":"2025120408000780000_2025.10.30.685561v2.5","doi-asserted-by":"publisher","DOI":"10.1038\/nature11049"},{"key":"2025120408000780000_2025.10.30.685561v2.6","doi-asserted-by":"publisher","DOI":"10.1038\/nature11082"},{"key":"2025120408000780000_2025.10.30.685561v2.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.11.021"},{"key":"2025120408000780000_2025.10.30.685561v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.04.085"},{"key":"2025120408000780000_2025.10.30.685561v2.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2019.05.001"},{"key":"2025120408000780000_2025.10.30.685561v2.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.072"},{"key":"2025120408000780000_2025.10.30.685561v2.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2022.09.003"},{"key":"2025120408000780000_2025.10.30.685561v2.12","doi-asserted-by":"crossref","unstructured":"Galitsyna A , Ulianov S V , Bykov NS , Veil M , Gao M , Perevoschikova K , et al. 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