{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:30:44Z","timestamp":1788874244189,"version":"build-2803163510"},"posted":{"date-parts":[[2025,11,3]]},"group-title":"Genomics","reference-count":52,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"crossref","award":["U24 MH133204"],"award-info":[{"award-number":["U24 MH133204"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["U24 NS132103"],"award-info":[{"award-number":["U24 NS132103"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132024"],"award-info":[{"award-number":["UG3 NS132024"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132061"],"award-info":[{"award-number":["UG3 NS132061"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132084"],"award-info":[{"award-number":["UG3 NS132084"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132105"],"award-info":[{"award-number":["UG3 NS132105"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132127"],"award-info":[{"award-number":["UG3 NS132127"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132128"],"award-info":[{"award-number":["UG3 NS132128"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132132"],"award-info":[{"award-number":["UG3 NS132132"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132134"],"award-info":[{"award-number":["UG3 NS132134"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132135"],"award-info":[{"award-number":["UG3 NS132135"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132136"],"award-info":[{"award-number":["UG3 NS132136"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132138"],"award-info":[{"award-number":["UG3 NS132138"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132139"],"award-info":[{"award-number":["UG3 NS132139"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132144"],"award-info":[{"award-number":["UG3 NS132144"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["UG3 NS132146"],"award-info":[{"award-number":["UG3 NS132146"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"crossref","award":["UM1 DA058219"],"award-info":[{"award-number":["UM1 DA058219"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"crossref","award":["UM1 DA058220"],"award-info":[{"award-number":["UM1 DA058220"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"crossref","award":["UM1 DA058229"],"award-info":[{"award-number":["UM1 DA058229"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"crossref","award":["UM1 DA058230"],"award-info":[{"award-number":["UM1 DA058230"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"crossref","award":["UM1 DA058235"],"award-info":[{"award-number":["UM1 DA058235"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"crossref","award":["UM1 DA058236"],"award-info":[{"award-number":["UM1 DA058236"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Single-cell DNA sequencing offers a powerful means of studying somatic mosaicism but requires careful analysis to mitigate DNA amplification-related artifacts. We performed primary template-directed amplification (PTA) and sequencing of 102 nuclei from postmortem lung and colon tissues of a 74-year-old male. Single-cell mutation burdens and spectra were validated by duplex sequencing and revealed heterogeneity across organs and cells, including signatures of APOBEC activity and tobacco exposure. Cells from both tissues exhibited chromosomal aneuploidies, loss of chromosome Y, and chromosomal rearrangements including rearrangements of the T-cell receptor loci indicative of T-cells. Shared embryonic mutations between cells enabled reconstruction of cellular ancestries from the zygote, which were validated by bulk sequencing. Collectively, we demonstrate a comprehensive approach for single-cell genomics that yields an expansive view of diverse somatic mutation types from development through aging across diverse tissues\u2014insights that are obscured in bulk sequencing and only partially captured by other single-cell methods.<\/jats:p>","DOI":"10.1101\/2025.10.31.685648","type":"posted-content","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T20:10:13Z","timestamp":1762200613000},"source":"Crossref","is-referenced-by-count":7,"title":["A comprehensive view of somatic mosaicism by single-cell DNA analysis"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6968-9043","authenticated-orcid":false,"given":"Lovelace J.","family":"Luquette","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5826-3554","authenticated-orcid":false,"given":"Tim H. H.","family":"Coorens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6498-6134","authenticated-orcid":false,"given":"Abhiram","family":"Natu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5839-9611","authenticated-orcid":false,"given":"Milovan","family":"Suvakov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3384-9049","authenticated-orcid":false,"given":"Ann","family":"Caplin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2699-4739","authenticated-orcid":false,"given":"Mee Sook","family":"Jun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6435-1227","authenticated-orcid":false,"given":"Alisa","family":"Mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Pelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4062-542X","authenticated-orcid":false,"given":"Lisa","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8577-9137","authenticated-orcid":false,"given":"Michele","family":"Berselli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4609-8627","authenticated-orcid":false,"given":"Sravya","family":"Bhamidipati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7050-2909","authenticated-orcid":false,"given":"Thomas","family":"Blanchard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4752-0749","authenticated-orcid":false,"given":"Joseph","family":"Brew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1911-9487","authenticated-orcid":false,"given":"Hye-Jung E.","family":"Chun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7186-6387","authenticated-orcid":false,"given":"Hyunbin","family":"Chun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5953-9495","authenticated-orcid":false,"given":"Mrunal K.","family":"Dehankar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1377-4696","authenticated-orcid":false,"given":"William C.","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"Furatero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3884-7720","authenticated-orcid":false,"given":"Christopher M.","family":"Grochowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7816-3278","authenticated-orcid":false,"given":"Yeongjun","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3872-0196","authenticated-orcid":false,"given":"Kavya","family":"Kottapalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8432-6803","authenticated-orcid":false,"given":"M. Kathryn","family":"Leonard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9787-8499","authenticated-orcid":false,"given":"Nam Seop","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0409-891X","authenticated-orcid":false,"given":"Tina","family":"Lindsay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0316-7506","authenticated-orcid":false,"given":"Sarah","family":"Nicholson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9522-2794","authenticated-orcid":false,"given":"Ivan","family":"Raimondi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8592-1444","authenticated-orcid":false,"given":"Alexi","family":"Runnels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1016-3917","authenticated-orcid":false,"given":"Constantijn","family":"Scharlee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9527-5718","authenticated-orcid":false,"given":"JaeEun","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3177-0812","authenticated-orcid":false,"given":"Alexander D.","family":"Veit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9399-6014","authenticated-orcid":false,"given":"Melissa","family":"VonDran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8056-9755","authenticated-orcid":false,"given":"Yifan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6979-6700","authenticated-orcid":false,"given":"Dennis J.","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4829-1428","authenticated-orcid":false,"given":"Yifan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"SMaHT Single Cell Focus Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3523-1736","authenticated-orcid":false,"given":"Thomas J.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4272-6283","authenticated-orcid":false,"given":"Kristin","family":"Ardlie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2433-633X","authenticated-orcid":false,"given":"Harsha","family":"Doddapaneni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6820-3404","authenticated-orcid":false,"given":"Robert","family":"Fulton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6038-8508","authenticated-orcid":false,"given":"Soren","family":"Germer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2346-9541","authenticated-orcid":false,"given":"Dan","family":"Landau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5742-5120","authenticated-orcid":false,"given":"Ji Won","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9378-960X","authenticated-orcid":false,"given":"Peter J.","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2167-981X","authenticated-orcid":false,"given":"Flora M.","family":"Vaccarino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0156-2238","authenticated-orcid":false,"given":"Christopher","family":"Walsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5405-6729","authenticated-orcid":false,"given":"Alexej","family":"Abyzov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025110515451375000_2025.10.31.685648v1.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa6806"},{"key":"2025110515451375000_2025.10.31.685648v1.2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau3879"},{"key":"2025110515451375000_2025.10.31.685648v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0497-0"},{"key":"2025110515451375000_2025.10.31.685648v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1672-7"},{"key":"2025110515451375000_2025.10.31.685648v1.5","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab1785"},{"key":"2025110515451375000_2025.10.31.685648v1.6","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao4426"},{"key":"2025110515451375000_2025.10.31.685648v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1961-1"},{"key":"2025110515451375000_2025.10.31.685648v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03477-4"},{"key":"2025110515451375000_2025.10.31.685648v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-023-01376-0"},{"key":"2025110515451375000_2025.10.31.685648v1.10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2013106118"},{"key":"2025110515451375000_2025.10.31.685648v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07532-8"},{"key":"2025110515451375000_2025.10.31.685648v1.12","doi-asserted-by":"crossref","unstructured":"Maslov, A. Y. et al. Single-molecule, quantitative detection of low-abundance somatic mutations by high-throughput sequencing. Sci Adv 8, eabm3259 (2022).","DOI":"10.1126\/sciadv.abm3259"},{"key":"2025110515451375000_2025.10.31.685648v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/nature19768"},{"key":"2025110515451375000_2025.10.31.685648v1.14","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03786-8"},{"key":"2025110515451375000_2025.10.31.685648v1.15","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe0981"},{"key":"2025110515451375000_2025.10.31.685648v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/nature11629"},{"key":"2025110515451375000_2025.10.31.685648v1.17","doi-asserted-by":"publisher","DOI":"10.1101\/gr.215517.116"},{"key":"2025110515451375000_2025.10.31.685648v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03822-7"},{"key":"2025110515451375000_2025.10.31.685648v1.19","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03790-y"},{"key":"2025110515451375000_2025.10.31.685648v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03836-1"},{"key":"2025110515451375000_2025.10.31.685648v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.02.025"},{"key":"2025110515451375000_2025.10.31.685648v1.22","doi-asserted-by":"publisher","DOI":"10.1126\/science.abm6222"},{"key":"2025110515451375000_2025.10.31.685648v1.23","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229164"},{"key":"2025110515451375000_2025.10.31.685648v1.24","doi-asserted-by":"publisher","DOI":"10.1126\/science.aak9787"},{"key":"2025110515451375000_2025.10.31.685648v1.25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2024176118"},{"key":"2025110515451375000_2025.10.31.685648v1.26","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-022-01180-2"},{"key":"2025110515451375000_2025.10.31.685648v1.27","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04640-1"},{"key":"2025110515451375000_2025.10.31.685648v1.28","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07292-5"},{"key":"2025110515451375000_2025.10.31.685648v1.29","doi-asserted-by":"crossref","unstructured":"Kalef-Ezra, E. et al. Single-cell somatic copy number variants in brain using different amplification methods and reference genomes. Commun Biol 7, 1288 (2024).","DOI":"10.1038\/s42003-024-06940-w"},{"key":"2025110515451375000_2025.10.31.685648v1.30","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1038\/s41593-025-01895-5","article-title":"Neuronal somatic mutations are increased in multiple sclerosis lesions","volume":"28","year":"2025","journal-title":"Nat Neurosci"},{"key":"2025110515451375000_2025.10.31.685648v1.31","doi-asserted-by":"crossref","unstructured":"Middelkamp, S. et al. Comprehensive single-cell genome analysis at nucleotide resolution using the PTA Analysis Toolbox. Cell Genom 3, 100389 (2023).","DOI":"10.1016\/j.xgen.2023.100389"},{"key":"2025110515451375000_2025.10.31.685648v1.32","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.yjmcc.2023.03.010","article-title":"High-Quality Nuclei Isolation from Postmortem Human Heart Muscle Tissues for Single-Cell Studies","volume":"179","year":"2023","journal-title":"J Mol Cell Cardiol"},{"key":"2025110515451375000_2025.10.31.685648v1.33","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09096-7"},{"key":"2025110515451375000_2025.10.31.685648v1.34","doi-asserted-by":"publisher","DOI":"10.1038\/nature12477"},{"key":"2025110515451375000_2025.10.31.685648v1.35","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1943-3"},{"key":"2025110515451375000_2025.10.31.685648v1.36","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-5843684\/v1"},{"key":"2025110515451375000_2025.10.31.685648v1.37","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-024-00962-8"},{"key":"2025110515451375000_2025.10.31.685648v1.38","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2921"},{"key":"2025110515451375000_2025.10.31.685648v1.39","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03345-1"},{"key":"2025110515451375000_2025.10.31.685648v1.40","doi-asserted-by":"crossref","unstructured":"Suvakov, M. , Panda, A. , Diesh, C. , Holmes, I. & Abyzov, A . CNVpytor: a tool for copy number variation detection and analysis from read depth and allele imbalance in whole-genome sequencing. Gigascience 10, giab074 (2021).","DOI":"10.1093\/gigascience\/giab074"},{"key":"2025110515451375000_2025.10.31.685648v1.41","doi-asserted-by":"crossref","unstructured":"Zhao, Y. et al. High-resolution detection of copy number alterations in single cells with HiScanner. Nat Commun 16, 5477 (2025).","DOI":"10.1038\/s41467-025-60446-5"},{"key":"2025110515451375000_2025.10.31.685648v1.42","doi-asserted-by":"publisher","DOI":"10.1038\/1971080a0"},{"key":"2025110515451375000_2025.10.31.685648v1.43","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv710"},{"key":"2025110515451375000_2025.10.31.685648v1.44","doi-asserted-by":"publisher","DOI":"10.1038\/nature09708"},{"key":"2025110515451375000_2025.10.31.685648v1.45","doi-asserted-by":"publisher","DOI":"10.1007\/BF00291876"},{"key":"2025110515451375000_2025.10.31.685648v1.46","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1969-6"},{"key":"2025110515451375000_2025.10.31.685648v1.47","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe1544"},{"key":"2025110515451375000_2025.10.31.685648v1.48","doi-asserted-by":"publisher","DOI":"10.1038\/nature14221"},{"key":"2025110515451375000_2025.10.31.685648v1.49","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"2025110515451375000_2025.10.31.685648v1.50","doi-asserted-by":"publisher","DOI":"10.1101\/115717"},{"key":"2025110515451375000_2025.10.31.685648v1.51","doi-asserted-by":"crossref","unstructured":"Delaneau, O. , Zagury, J.-F. , Robinson, M. R. , Marchini, J. L. & Dermitzakis, E. T . Accurate, scalable and integrative haplotype estimation. Nat Commun 10, 5436 (2019).","DOI":"10.1038\/s41467-019-13225-y"},{"key":"2025110515451375000_2025.10.31.685648v1.52","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2016.09.005"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.10.31.685648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:56:57Z","timestamp":1768471017000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.10.31.685648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,3]]},"references-count":52,"URL":"https:\/\/doi.org\/10.1101\/2025.10.31.685648","relation":{},"subject":[],"published":{"date-parts":[[2025,11,3]]},"subtype":"preprint"}}