{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:32Z","timestamp":1785402152356,"version":"3.56.0"},"posted":{"date-parts":[[2025,11,4]]},"group-title":"Immunology","reference-count":15,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"award":["ARISE-PP-FA-056"],"award-info":[{"award-number":["ARISE-PP-FA-056"]}],"id":[{"id":"https:\/\/ror.org\/05px9k635","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100013043","name":"Yale Center for Clinical Investigation","doi-asserted-by":"crossref","award":["TR001864"],"award-info":[{"award-number":["TR001864"]}],"id":[{"id":"10.13039\/100013043","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI168238"],"award-info":[{"award-number":["R01 AI168238"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"award":["STARS Fellowship"],"award-info":[{"award-number":["STARS Fellowship"]}],"id":[{"id":"https:\/\/ror.org\/03v76x132","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  PfCyRPA and PfRIPR are promising next-generation malaria blood-stage vaccine candidate antigens that play an essential role in erythrocyte invasion of\n                  <jats:italic>Plasmodium falciparum<\/jats:italic>\n                  . CyRPA and RIPR orthologs are present in all human-infecting\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  species, suggesting the potential for a cross-species vaccine. Using Growth Inhibition Assays (GIA), this study investigates seven anti-PfCyRPA and three anti-PfRIPR monoclonal antibodies targeting\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  for their inhibitory activity against\n                  <jats:italic>P. knowlesi<\/jats:italic>\n                  , a non-falciparum species that contributes to a significant burden of zoonotic disease in South-East Asia, shares some biological features with\n                  <jats:italic>Plasmodium vivax<\/jats:italic>\n                  , and has a robust\n                  <jats:italic>in vitro<\/jats:italic>\n                  culture system. Despite their efficacy against\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  and partially conserved epitopes, these antibodies exhibited minimal inhibition of\n                  <jats:italic>P. knowlesi<\/jats:italic>\n                  . Understanding the antigenic diversity and immune mechanisms across\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  species is critical for advancing pan-species vaccine strategies.\n                <\/jats:p>","DOI":"10.1101\/2025.11.03.682341","type":"posted-content","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T04:05:13Z","timestamp":1762315513000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the potential of cross-species neutralization of anti-PfCyRPA and anti-PfRIPR Monoclonal Antibodies"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8472-7130","authenticated-orcid":false,"given":"Noemi","family":"Guerra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7200-7023","authenticated-orcid":false,"given":"Kelly A.","family":"Hagadorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2499-8860","authenticated-orcid":false,"given":"Megha","family":"Nair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7086-4726","authenticated-orcid":false,"given":"Laty Gaye","family":"Thiam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3078-8076","authenticated-orcid":false,"given":"Barnabas G.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5411-7883","authenticated-orcid":false,"given":"Kirsty","family":"McHugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2230-6364","authenticated-orcid":false,"given":"Dimitra","family":"Pipini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8917-6237","authenticated-orcid":false,"given":"Julie","family":"Healer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Masendycz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5145-9004","authenticated-orcid":false,"given":"Alan F.","family":"Cowman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9415-1357","authenticated-orcid":false,"given":"Simon J.","family":"Draper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1159-760X","authenticated-orcid":false,"given":"Amy K.","family":"Bei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025110700450411000_2025.11.03.682341v1.1","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1038\/s41564-022-01261-2","article-title":"Pcrcr complex is essential for invasion of human erythrocytes by plasmodium falciparum","volume":"7","year":"2022","journal-title":"Nat Microbiol"},{"key":"2025110700450411000_2025.11.03.682341v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00752-7"},{"key":"2025110700450411000_2025.11.03.682341v1.3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007809"},{"key":"2025110700450411000_2025.11.03.682341v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nature21038"},{"key":"2025110700450411000_2025.11.03.682341v1.5","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1038\/s41467-022-28601-4","article-title":"Heterotypic interactions drive antibody synergy against a malaria vaccine candidate","volume":"13","year":"2022","journal-title":"Nat Commun"},{"key":"2025110700450411000_2025.11.03.682341v1.6","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13030"},{"key":"2025110700450411000_2025.11.03.682341v1.7","doi-asserted-by":"crossref","unstructured":"Chen, L. et al. 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