{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:54:54Z","timestamp":1785434094475,"version":"3.56.0"},"posted":{"date-parts":[[2025,11,5]]},"group-title":"Cell Biology","reference-count":0,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["DE026172"],"award-info":[{"award-number":["DE026172"]}],"id":[{"id":"https:\/\/ror.org\/004a2wv92","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DE032825"],"award-info":[{"award-number":["DE032825"]}],"id":[{"id":"https:\/\/ror.org\/004a2wv92","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DE032515"],"award-info":[{"award-number":["DE032515"]}],"id":[{"id":"https:\/\/ror.org\/004a2wv92","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DE032742"],"award-info":[{"award-number":["DE032742"]}],"id":[{"id":"https:\/\/ror.org\/004a2wv92","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DE031181"],"award-info":[{"award-number":["DE031181"]}],"id":[{"id":"https:\/\/ror.org\/004a2wv92","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Center for Advancing Translational Sciences","award":["TL1R002368"],"award-info":[{"award-number":["TL1R002368"]}]},{"name":"National Institute of General Medical Sciences","award":["GM122731"],"award-info":[{"award-number":["GM122731"]}]},{"name":"National Institute of General Medical Sciences","award":["GM103418"],"award-info":[{"award-number":["GM103418"]}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["HD090216"],"award-info":[{"award-number":["HD090216"]}]},{"name":"Office of Research Infrastructure Programs","award":["OD023625"],"award-info":[{"award-number":["OD023625"]}]},{"name":"Office of Research Infrastructure Programs","award":["OD032207"],"award-info":[{"award-number":["OD032207"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,5]]},"abstract":"<jats:p>\n                  Cilium formation and actin cytoskeleton dynamics are interconnected, with evidence showing that elevated filamentous actin (F-actin) negatively regulates primary cilia length. Loss of the cytoskeletal protein SPECC1L, which itself does not localize to cilia, leads to increased F-actin and shortened cilia. Depolymerizing F-actin in\n                  <jats:italic>Specc1l<\/jats:italic>\n                  mutant cells restored cilia lengths, substantiating this inverse relationship. In cells harboring a\n                  <jats:italic>Specc1l<\/jats:italic>\n                  allele lacking only the coiled-coil domain 2, intracellular regions with both elevated and reduced F-actin were observed together with cilia shortening. Notably, F-actin was decreased at the ciliary base, suggesting that a different F-actin subpopulation contributes to the inverse relationship. We also identified a genetic interaction between\n                  <jats:italic>Specc1l<\/jats:italic>\n                  and\n                  <jats:italic>Thm1<\/jats:italic>\n                  , which encodes an intraflagellar transport-A (IFT-A) protein. Double or compound heterozygotes for\n                  <jats:italic>Specc1l<\/jats:italic>\n                  and\n                  <jats:italic>Thm1<\/jats:italic>\n                  exhibited a higher penetrance of cleft palate compared to\n                  <jats:italic>Specc1l<\/jats:italic>\n                  heterozygotes alone. Together, these findings reveal a role for SPECC1L in cytoskeletal regulation of ciliogenesis affecting palate development.\n                <\/jats:p>","DOI":"10.1101\/2025.11.03.686369","type":"posted-content","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T17:45:18Z","timestamp":1762364718000},"source":"Crossref","is-referenced-by-count":1,"title":["Genetic interaction of\n                  <i>Specc1l<\/i>\n                  and\n                  <i>Thm1<\/i>\n                  reveals cytoskeletal - ciliary crosstalk"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2063-5420","authenticated-orcid":false,"given":"Brittany M","family":"Hufft-Martinez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4727-5963","authenticated-orcid":false,"given":"Dana N","family":"Thalman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An J","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy P","family":"Goering","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3675-3082","authenticated-orcid":false,"given":"Marta","family":"Stetsiv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Moedritzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry H","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4619-4376","authenticated-orcid":false,"given":"Pamela V","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6250-6651","authenticated-orcid":false,"given":"Irfan","family":"Saadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.11.03.686369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:57:56Z","timestamp":1768471076000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.11.03.686369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1101\/2025.11.03.686369","relation":{},"subject":[],"published":{"date-parts":[[2025,11,5]]},"subtype":"preprint"}}