{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:19:58Z","timestamp":1785428398139,"version":"3.56.0"},"posted":{"date-parts":[[2025,11,5]]},"group-title":"Bioengineering","reference-count":31,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R44GM154516"],"award-info":[{"award-number":["R44GM154516"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  By optically sensing mid-infrared absorption through a visible probe beam, mid-infrared photothermal (MIP) microscopy has emerged as a powerful tool for chemical imaging with micromolar sensitivity and submicron spatial resolution. The adoption of spatially multiplexed camera-based widefield detection further enhanced the imaging speed. However, current widefield MIP systems suffer from a small field of view (FOV)\u2014typically tens of square micrometers, which constrains their utility in large-area tissue imaging applications. Here, we report a laser-scan MIP mesoscope that achieves millimeter-scale FOV while preserving submicron resolution. By leveraging an all-reflective laser scanning architecture, low-magnification and medium numerical-aperture objectives, and a defocused signal collection scheme, our MIP mesoscope achieves a 1.2 \u00d7 1.2 mm\n                  <jats:sup>2<\/jats:sup>\n                  FOV, 650 nm lateral resolution, and microsecond-scale pixel dwell time. In vivo chemical imaging of whole\n                  <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                  and high-throughput detection of beta-amyloids in both mouse and human brain tissues associated with Alzheimer\u2019s disease are demonstrated.\n                <\/jats:p>","DOI":"10.1101\/2025.11.03.686410","type":"posted-content","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T18:20:19Z","timestamp":1762366819000},"source":"Crossref","is-referenced-by-count":0,"title":["Mid-Infrared Photothermal Mesoscopy with Millimeter Field of View and Sub-micron Spatial Resolution"],"prefix":"10.64898","author":[{"given":"Rong","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaze","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany","family":"Weinberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haonan","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oxana","family":"Klementieva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Xin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025110710351221000_2025.11.03.686410v1.1","doi-asserted-by":"publisher","DOI":"10.1366\/12-06801"},{"key":"2025110710351221000_2025.11.03.686410v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.110"},{"key":"2025110710351221000_2025.11.03.686410v1.3","first-page":"269A","volume":"60","year":"1988","journal-title":"Emerging techniques in biophysical FT-IR"},{"key":"2025110710351221000_2025.11.03.686410v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40740-w"},{"key":"2025110710351221000_2025.11.03.686410v1.5","unstructured":"Eid, M. 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Microglial mechanisms drive amyloid-\u03b2 clearance in immunized patients with Alzheimer\u2019s disease. 1\u201313 (2025)."}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.11.03.686410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:58:01Z","timestamp":1768471081000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.11.03.686410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,5]]},"references-count":31,"URL":"https:\/\/doi.org\/10.1101\/2025.11.03.686410","relation":{},"subject":[],"published":{"date-parts":[[2025,11,5]]},"subtype":"preprint"}}