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One member, galectin-8, preferentially binds galactose that contains a terminal sulfate. Aberrant expression and secretion of sulfated glycosylation epitopes, such as 3\u2019-Sulfo-Le\n                  <jats:sup>A\/C<\/jats:sup>\n                  , is a feature of high-risk human foregut metaplasias. In addition, recent work has demonstrated that 3\u2019-Sulfo-Le\n                  <jats:sup>C<\/jats:sup>\n                  is a marker of mature murine zymogenic chief cells of the stomach and that 3\u2019-Sulfo-Le\n                  <jats:sup>C<\/jats:sup>\n                  epitope is secreted via cathartocytosis during the cellular transition to a metaplastic state. Based on those findings, we used\n                  <jats:italic>\n                    Lgals8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                  <\/jats:italic>\n                  mice, to determine whether galectin-8 might play a role in chief cell homeostasis. We observed delayed gastric differentiation in the\n                  <jats:italic>\n                    Lgals8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                  <\/jats:italic>\n                  mice and discovered that this phenotype was due to an unappreciated deletion of\n                  <jats:italic>Mmrn1<\/jats:italic>\n                  and\n                  <jats:italic>Snca<\/jats:italic>\n                  in the\n                  <jats:italic>\n                    Lgals8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                  <\/jats:italic>\n                  line. We show that multimerin-1 tempers WNT stimulation of the gastric corpus at an early age, as evidenced by nuclear beta-catenin staining and proliferation throughout the gland. Because multimerin-1 is synthesized and secreted from endothelial cells and not from the epithelial compartment, these data uncover a role for mesodermal cells in epithelial developmental and maturation of the mouse stomach. As prior studies have suggested galectin-8 and multimerin-1 have overlapping functions albeit, divergent with respect to bone, future studies using pure knockouts are necessary to refine these phenotypes.\n                <\/jats:p>","DOI":"10.1101\/2025.11.04.685347","type":"posted-content","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:55:19Z","timestamp":1762376119000},"source":"Crossref","is-referenced-by-count":1,"title":["Multimerin1, not Galectin-8, Promotes Gastric Chief Cell Differentiation by Tempering WNT 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