{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:27:42Z","timestamp":1773757662414,"version":"3.50.1"},"posted":{"date-parts":[[2025,11,6]]},"group-title":"Immunology","reference-count":113,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health grant","doi-asserted-by":"crossref","award":["1P01AI143565"],"award-info":[{"award-number":["1P01AI143565"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health grant","doi-asserted-by":"crossref","award":["T32 DK101003"],"award-info":[{"award-number":["T32 DK101003"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,14]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Memory CD8\u207a T cells provide durable protection against recurrent infection and cancer, but how distinct memory fates are specified remains unclear. Here, we define the functional landscape of 242 murine CD8\u207a TCR\u03b1\u03b2 clonotypes specific for influenza NP\n                  <jats:sub>366\u2013374<\/jats:sub>\n                  \/H-2D\u1d47 ligand (pMHC) by integrating single-cell transcriptomics and TCR sequencing with force-dependent biophysics, structural analysis, and in vivo investigation of memory development. Fate essentially tracks with TCR mechanotransduction: T\n                  <jats:sub>CM<\/jats:sub>\n                  -associated clonotypes preferentially engage pMHC through TCR\u03b2, whereas T\n                  <jats:sub>EM<\/jats:sub>\n                  -associated clonotypes preferentially engage through TCR\u03b1. Clonotypes with balanced, bipolar signaling generate both memory subsets, optimize expansion, and display broad heterosubtypic crossreactivity but with exhaustion susceptibility. In contrast, diverse T\n                  <jats:sub>CM<\/jats:sub>\n                  clonotypes are largely clonal singlets with considerable mutant-epitope recognition in aggregate and stemness preservation, revealing complementary strategies for immunodefense coverage. Atomistically, subtle pMHC contact differences and load transmission through the TCR holoreceptor appear to tune phosphorylation, memory differentiation, and functional durability, features applicable to adoptive T-cell immunotherapies.\n                <\/jats:p>","DOI":"10.1101\/2025.11.05.686789","type":"posted-content","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T01:05:28Z","timestamp":1762477528000},"source":"Crossref","is-referenced-by-count":0,"title":["Atomistic TCR-ligand interactions shape memory T-cell differentiation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7672-9664","authenticated-orcid":false,"given":"Aoi","family":"Akitsu","sequence":"first","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4003-7903","authenticated-orcid":false,"given":"Kemin","family":"Tan","sequence":"additional","affiliation":[{"name":"Structural Biology Center, X-Ray Science Division, Advanced Photon Source, Argonne National Laboratory; Lemont, IL 60439, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2087-9468","authenticated-orcid":false,"given":"Robert J.","family":"Mallis","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"},{"name":"Department of Dermatology, Harvard Medical School; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6902-1032","authenticated-orcid":false,"given":"Matthew A.","family":"Booker","sequence":"additional","affiliation":[{"name":"Department of Informatics and Analytics, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9478-9609","authenticated-orcid":false,"given":"Jonathan S.","family":"Duke-Cohan","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4406-9179","authenticated-orcid":false,"given":"Kristine N.","family":"Brazin","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3439-4874","authenticated-orcid":false,"given":"Evan H.","family":"Kirkpatrick","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Vanderbilt University; Nashville, TN 37212, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2821-1398","authenticated-orcid":false,"given":"Andrew N.","family":"Parkins","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2909-3484","authenticated-orcid":false,"given":"Amelia G.","family":"Seabury","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Vanderbilt University; Nashville, TN 37212, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5502-4363","authenticated-orcid":false,"given":"Sarita","family":"Aryal","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8555-2906","authenticated-orcid":false,"given":"Vincenzo","family":"Cinella","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan J.","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2858-9211","authenticated-orcid":false,"given":"Kaveri I.","family":"Uberoy","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6952-955X","authenticated-orcid":false,"given":"Jenna K.","family":"Koenig","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Biddle","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2718-6525","authenticated-orcid":false,"given":"Cameron M.","family":"Messier","sequence":"additional","affiliation":[{"name":"Belfer Center for Applied Cancer Science, Dana-Farber Cancer Institute; Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9510-0036","authenticated-orcid":false,"given":"Patrick H.","family":"Lizotte","sequence":"additional","affiliation":[{"name":"Belfer Center for Applied Cancer Science, Dana-Farber Cancer Institute; Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9134-8808","authenticated-orcid":false,"given":"Michael Y.","family":"Tolstorukov","sequence":"additional","affiliation":[{"name":"Department of Informatics and Analytics, Dana-Farber Cancer Institute; Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7514-3186","authenticated-orcid":false,"given":"Wonmuk","family":"Hwang","sequence":"additional","affiliation":[{"name":"Departments of Biomedical Engineering, Materials Science & Engineering, Physics & Astronomy, Texas A&M University; College Station, TX 77843, USA"},{"name":"Center for AI and Natural Sciences, Korea Institute for Advanced Study; Seoul 02455, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J.","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Vanderbilt University; Nashville, TN 37212, USA"},{"name":"Department of Molecular Physiology and Biophysics; Vanderbilt University School of Medicine; Nashville, TN 37232, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1048-5526","authenticated-orcid":false,"given":"Ellis L.","family":"Reinherz","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute; Boston, MA 02115, USA"},{"name":"Department of Medicine, Harvard Medical School; Boston; MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026031704500562000_2025.11.05.686789v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3307"},{"key":"2026031704500562000_2025.11.05.686789v2.2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.11.5535-5545.2004"},{"key":"2026031704500562000_2025.11.05.686789v2.3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104702"},{"key":"2026031704500562000_2025.11.05.686789v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.02.010"},{"key":"2026031704500562000_2025.11.05.686789v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.08.002"},{"key":"2026031704500562000_2025.11.05.686789v2.6","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201150"},{"key":"2026031704500562000_2025.11.05.686789v2.7","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2158"},{"key":"2026031704500562000_2025.11.05.686789v2.8","doi-asserted-by":"publisher","DOI":"10.1038\/ni889"},{"key":"2026031704500562000_2025.11.05.686789v2.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914127107"},{"key":"2026031704500562000_2025.11.05.686789v2.10","doi-asserted-by":"publisher","DOI":"10.1038\/ni1403"},{"key":"2026031704500562000_2025.11.05.686789v2.11","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171584"},{"key":"2026031704500562000_2025.11.05.686789v2.12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00353"},{"key":"2026031704500562000_2025.11.05.686789v2.13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01150.x"},{"key":"2026031704500562000_2025.11.05.686789v2.14","doi-asserted-by":"publisher","DOI":"10.1038\/ni1227"},{"key":"2026031704500562000_2025.11.05.686789v2.15","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000492"},{"key":"2026031704500562000_2025.11.05.686789v2.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.04.013"},{"key":"2026031704500562000_2025.11.05.686789v2.17","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00617"},{"key":"2026031704500562000_2025.11.05.686789v2.18","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-06-292748"},{"key":"2026031704500562000_2025.11.05.686789v2.19","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1901167"},{"key":"2026031704500562000_2025.11.05.686789v2.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.09.007"},{"key":"2026031704500562000_2025.11.05.686789v2.21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1424829112"},{"key":"2026031704500562000_2025.11.05.686789v2.22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703559114"},{"key":"2026031704500562000_2025.11.05.686789v2.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2018.05.005"},{"key":"2026031704500562000_2025.11.05.686789v2.24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2005899117"},{"key":"2026031704500562000_2025.11.05.686789v2.25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.052712"},{"key":"2026031704500562000_2025.11.05.686789v2.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.02.053"},{"key":"2026031704500562000_2025.11.05.686789v2.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.12.018"},{"key":"2026031704500562000_2025.11.05.686789v2.28","doi-asserted-by":"publisher","DOI":"10.1126\/science.abl5282"},{"key":"2026031704500562000_2025.11.05.686789v2.29","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13432"},{"key":"2026031704500562000_2025.11.05.686789v2.30","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ado4313"},{"key":"2026031704500562000_2025.11.05.686789v2.31","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202302967"},{"key":"2026031704500562000_2025.11.05.686789v2.32","doi-asserted-by":"publisher","DOI":"10.1038\/nature22383"},{"key":"2026031704500562000_2025.11.05.686789v2.33","doi-asserted-by":"crossref","unstructured":"Travaglino, S. , Kazemipour Ashkezari, A.H. , Li, M. , Watson, V.E. , Cong, P. , Doudy, L. , Choi, H.-K. , and Zhu, C. (2025). Evaluating the effects of CD8\/CD4 on T cell function in terms of TCR\u2013pMHC\u2013coreceptor catch and slip bonds. bioRxiv, 2025.2012. 2001.691627.","DOI":"10.64898\/2025.12.01.691627"},{"key":"2026031704500562000_2025.11.05.686789v2.34","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050137"},{"key":"2026031704500562000_2025.11.05.686789v2.35","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3350"},{"key":"2026031704500562000_2025.11.05.686789v2.36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603106113"},{"key":"2026031704500562000_2025.11.05.686789v2.37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010583"},{"key":"2026031704500562000_2025.11.05.686789v2.38","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90171-6"},{"key":"2026031704500562000_2025.11.05.686789v2.39","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5285.209"},{"key":"2026031704500562000_2025.11.05.686789v2.40","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.104280"},{"key":"2026031704500562000_2025.11.05.686789v2.41","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.91881"},{"key":"2026031704500562000_2025.11.05.686789v2.42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.20.9166"},{"key":"2026031704500562000_2025.11.05.686789v2.43","doi-asserted-by":"publisher","DOI":"10.1126\/science.aae0491"},{"key":"2026031704500562000_2025.11.05.686789v2.44","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.11.014"},{"key":"2026031704500562000_2025.11.05.686789v2.45","doi-asserted-by":"publisher","DOI":"10.1172\/JCI148546"},{"key":"2026031704500562000_2025.11.05.686789v2.46","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56931-6"},{"key":"2026031704500562000_2025.11.05.686789v2.47","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53593-8"},{"key":"2026031704500562000_2025.11.05.686789v2.48","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-024-00715-7"},{"key":"2026031704500562000_2025.11.05.686789v2.49","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10661-8"},{"key":"2026031704500562000_2025.11.05.686789v2.50","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12646"},{"key":"2026031704500562000_2025.11.05.686789v2.51","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2025.02.001"},{"key":"2026031704500562000_2025.11.05.686789v2.52","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-66939-7"},{"key":"2026031704500562000_2025.11.05.686789v2.53","doi-asserted-by":"publisher","DOI":"10.1038\/nri1636"},{"key":"2026031704500562000_2025.11.05.686789v2.54","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.752865"},{"key":"2026031704500562000_2025.11.05.686789v2.55","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.813493"},{"key":"2026031704500562000_2025.11.05.686789v2.56","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe0918"},{"key":"2026031704500562000_2025.11.05.686789v2.57","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05555-7"},{"key":"2026031704500562000_2025.11.05.686789v2.58","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01194-2"},{"key":"2026031704500562000_2025.11.05.686789v2.59","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2012.02.002"},{"key":"2026031704500562000_2025.11.05.686789v2.60","doi-asserted-by":"publisher","DOI":"10.1038\/ni1009"},{"key":"2026031704500562000_2025.11.05.686789v2.61","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2086"},{"key":"2026031704500562000_2025.11.05.686789v2.62","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290345"},{"key":"2026031704500562000_2025.11.05.686789v2.63","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1103481"},{"key":"2026031704500562000_2025.11.05.686789v2.64","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171708"},{"key":"2026031704500562000_2025.11.05.686789v2.65","doi-asserted-by":"publisher","DOI":"10.1038\/nature02238"},{"key":"2026031704500562000_2025.11.05.686789v2.66","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070051"},{"key":"2026031704500562000_2025.11.05.686789v2.67","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80080-1"},{"key":"2026031704500562000_2025.11.05.686789v2.68","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2025.09.005"},{"key":"2026031704500562000_2025.11.05.686789v2.69","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000356"},{"key":"2026031704500562000_2025.11.05.686789v2.70","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13722-0"},{"key":"2026031704500562000_2025.11.05.686789v2.71","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.92"},{"key":"2026031704500562000_2025.11.05.686789v2.72","doi-asserted-by":"publisher","DOI":"10.1126\/science.abm1581"},{"key":"2026031704500562000_2025.11.05.686789v2.73","doi-asserted-by":"publisher","DOI":"10.1126\/science.adv0422"},{"key":"2026031704500562000_2025.11.05.686789v2.74","doi-asserted-by":"publisher","DOI":"10.1126\/science.adv0185"},{"key":"2026031704500562000_2025.11.05.686789v2.75","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.005"},{"key":"2026031704500562000_2025.11.05.686789v2.76","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14049"},{"key":"2026031704500562000_2025.11.05.686789v2.77","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"2026031704500562000_2025.11.05.686789v2.78","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2018.11.005"},{"key":"2026031704500562000_2025.11.05.686789v2.79","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"2026031704500562000_2025.11.05.686789v2.80","doi-asserted-by":"publisher","DOI":"10.21105\/joss.00861"},{"key":"2026031704500562000_2025.11.05.686789v2.81","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07956-7"},{"key":"2026031704500562000_2025.11.05.686789v2.82","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-024-01020-z"},{"key":"2026031704500562000_2025.11.05.686789v2.83","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0414-6"},{"key":"2026031704500562000_2025.11.05.686789v2.84","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02303-9"},{"key":"2026031704500562000_2025.11.05.686789v2.85","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.68605"},{"key":"2026031704500562000_2025.11.05.686789v2.86","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.61"},{"key":"2026031704500562000_2025.11.05.686789v2.87","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3386"},{"key":"2026031704500562000_2025.11.05.686789v2.88","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11408"},{"key":"2026031704500562000_2025.11.05.686789v2.89","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.52.33639"},{"key":"2026031704500562000_2025.11.05.686789v2.90","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2011.08.005"},{"key":"2026031704500562000_2025.11.05.686789v2.91","doi-asserted-by":"publisher","DOI":"10.1016\/0960-9822(93)90063-T"},{"key":"2026031704500562000_2025.11.05.686789v2.92","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2009.08.009"},{"key":"2026031704500562000_2025.11.05.686789v2.93","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4950"},{"key":"2026031704500562000_2025.11.05.686789v2.94","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003647"},{"key":"2026031704500562000_2025.11.05.686789v2.95","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzg087"},{"key":"2026031704500562000_2025.11.05.686789v2.96","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41502"},{"key":"2026031704500562000_2025.11.05.686789v2.97","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106851108"},{"key":"2026031704500562000_2025.11.05.686789v2.98","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.212519299"},{"key":"2026031704500562000_2025.11.05.686789v2.99","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200109)31:9<2791::AID-IMMU2791>3.0.CO;2-X"},{"key":"2026031704500562000_2025.11.05.686789v2.100","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1504971112"},{"key":"2026031704500562000_2025.11.05.686789v2.101","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"2026031704500562000_2025.11.05.686789v2.102","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"2026031704500562000_2025.11.05.686789v2.103","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2026031704500562000_2025.11.05.686789v2.104","doi-asserted-by":"publisher","DOI":"10.1261\/rna.074922.120"},{"key":"2026031704500562000_2025.11.05.686789v2.105","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444906019949"},{"key":"2026031704500562000_2025.11.05.686789v2.106","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042589"},{"key":"2026031704500562000_2025.11.05.686789v2.107","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"2026031704500562000_2025.11.05.686789v2.108","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444912001308"},{"key":"2026031704500562000_2025.11.05.686789v2.109","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"},{"key":"2026031704500562000_2025.11.05.686789v2.110","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.4c04100"},{"key":"2026031704500562000_2025.11.05.686789v2.111","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005659"},{"key":"2026031704500562000_2025.11.05.686789v2.112","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20832"},{"key":"2026031704500562000_2025.11.05.686789v2.113","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.23354"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.11.05.686789","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T11:50:14Z","timestamp":1773748214000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.11.05.686789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,6]]},"references-count":113,"URL":"https:\/\/doi.org\/10.1101\/2025.11.05.686789","relation":{},"subject":[],"published":{"date-parts":[[2025,11,6]]},"subtype":"preprint"}}