{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:36:23Z","timestamp":1790786183797,"version":"4.1.0"},"posted":{"date-parts":[[2025,11,7]]},"group-title":"Microbiology","reference-count":58,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["U19 AI162584"],"award-info":[{"award-number":["U19 AI162584"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 AI165573"],"award-info":[{"award-number":["R01 AI165573"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 AI049313"],"award-info":[{"award-number":["R01 AI049313"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 AI125517"],"award-info":[{"award-number":["R01 AI125517"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 AI130236"],"award-info":[{"award-number":["R01 AI130236"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R21 AI185955"],"award-info":[{"award-number":["R21 AI185955"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Unlike gene-first approaches to understanding bacterial pathogenesis, molecule-forward discovery can uncover unexpected chemical diversity. Here, new lipidomic analytical methods and quality metrics defined the large scope of unknown lipids in the world\u2019s deadliest pathogen,\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  (Mtb). This map allowed rapid discovery of Mtb lysyldiacylglycerol linked to the biosynthetic gene\n                  <jats:italic>lysX,<\/jats:italic>\n                  which controls\n                  <jats:italic>in vivo<\/jats:italic>\n                  infection outcomes in moth larvae, mice, guinea pigs, and here, zebrafish. A broader search for orthologous lysyltransferase domains identified the\n                  <jats:italic>Staphylococcus aureus<\/jats:italic>\n                  virulence gene\n                  <jats:italic>mprF<\/jats:italic>\n                  , where the same lipoamino acid was shown to be a previously unknown biosynthetic product. Thus, lipidomic mapping showed that the cell envelope composition of well-studied bacterial pathogens remains substantially unsolved and offers a new way to generate lists of discoverable lipids to accelerate molecular discovery.\n                <\/jats:p>","DOI":"10.1101\/2025.11.06.685907","type":"posted-content","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T03:30:14Z","timestamp":1762572614000},"source":"Crossref","is-referenced-by-count":1,"title":["Mapping unsolved lipidomes accelerates lipid discovery in major bacterial pathogens"],"prefix":"10.64898","author":[{"given":"Yashodhan M.","family":"Nair","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruna R.","family":"Menon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonghao","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel R. 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