{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T20:32:54Z","timestamp":1785875574377,"version":"3.56.0"},"posted":{"date-parts":[[2025,11,7]]},"group-title":"Immunology","reference-count":38,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"NIH NIDDK","award":["DK133393"],"award-info":[{"award-number":["DK133393"]}]},{"name":"NIH NIAID","award":["AI038339"],"award-info":[{"award-number":["AI038339"]}]},{"id":[{"id":"https:\/\/ror.org\/02xhk2825","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Searle Scholar's Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,22]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  We investigated whether T cell responses and antigen-presenting cell (APC) requirements in gut-draining lymph nodes differ by antigen source, diet\n                  <jats:italic>versus<\/jats:italic>\n                  epithelium. Using mice fed ovalbumin (OVA) or expressing secreted (s), cytosolic (c), or transmembrane (tm) epithelial OVA, we compared OVA-specific T cell fates. At baseline and after reovirus infection, T cell responses were comparable across models. However, helminth infection induced Th2 cell polarization in sOVA and tmOVA but not cOVA or OVA-fed mice. BATF3\u207a APCs were indispensable for CD4\u207a T cell proliferation only in cOVA mice yet drove Treg cell differentiation across all epithelial OVA models. In contrast, antigen presentation by ROR\u03b3t\u207aMHC-II\u207a APCs was exclusively required for Treg cell induction by dietary OVA. These distinct APC dependencies correlated with susceptibility to pathology elicited by dietary\n                  <jats:italic>versus<\/jats:italic>\n                  epithelial self-antigens. Thus, antigen origin and presentation context are integrated to shape T cell fate, a new framework for predicting gut immune outcomes.\n                <\/jats:p>","DOI":"10.1101\/2025.11.06.687030","type":"posted-content","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T04:30:17Z","timestamp":1762576217000},"source":"Crossref","is-referenced-by-count":4,"title":["T cell fate is dictated by different antigen presenting cells in response to dietary\n                  <i>versus<\/i>\n                  gut epithelial self-antigen"],"prefix":"10.64898","author":[{"given":"Yixuan D.","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Schaffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwen M.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay L.","family":"Fiske","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Macy R.","family":"Komnick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terence S.","family":"Dermody","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Esterh\u00e1zy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025112423150912000_2025.11.06.687030v3.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1125-3"},{"key":"2025112423150912000_2025.11.06.687030v3.2","doi-asserted-by":"crossref","unstructured":"Canesso, M. 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