{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:17:31Z","timestamp":1785424651297,"version":"3.56.0"},"posted":{"date-parts":[[2025,11,13]]},"group-title":"Neuroscience","reference-count":20,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["RF1NS136995"],"award-info":[{"award-number":["RF1NS136995"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01AG087513"],"award-info":[{"award-number":["R01AG087513"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["T32AG058507"],"award-info":[{"award-number":["T32AG058507"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01MH134004"],"award-info":[{"award-number":["R01MH134004"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["S10OD032285"],"award-info":[{"award-number":["S10OD032285"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AARG-23-1149996"],"award-info":[{"award-number":["AARG-23-1149996"]}],"id":[{"id":"https:\/\/ror.org\/0375f4d26","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Tractometry allows quantitative analysis of white matter microstructure along the brain\u2019s fiber tracts, but the impact of study design parameters\u2014such as sample size and along-tract resolution\u2014on sensitivity and specificity is not well understood. In this study, we conducted tractometry bootstrap analysis using linear-mixed models across four diffusion tensor imaging (DTI) metrics to systematically evaluate how these factors affect the detection of dementia- and amyloidrelated effects. While coarser along-tract segments yield greater sensitivity and higher mean effect sizes, finer segments tend to produce higher peak effect sizes, revealing more spatially localized effects. Dementia-related effects were more widespread and detectable with fewer subjects, whereas amyloid-related effects were more subtle and localized, requiring larger cohorts to detect them. These findings highlight that tractometry offers improved spatial specificity and can reliably detect small, fine-scale effects, but study design should be tailored to specific research questions, considering the expected spatial extent and magnitude of effects, to optimize sample size efficiency and interpretability.<\/jats:p>","DOI":"10.1101\/2025.11.11.687878","type":"posted-content","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T17:35:32Z","timestamp":1763055332000},"source":"Crossref","is-referenced-by-count":2,"title":["Evaluating Sample-Size Efficiency and Sensitivity of Tractometry in Alzheimer\u2019s Disease"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1015-4209","authenticated-orcid":false,"given":"Yixue","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio E.","family":"Villal\u00f3n-Reina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1443-8786","authenticated-orcid":false,"given":"Iyad Ba","family":"Gari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan Davis","family":"Alibrando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talia M.","family":"Nir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neda","family":"Jahanshad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bramsh Q.","family":"Chandio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4720-8867","authenticated-orcid":false,"given":"Paul M.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025111623300845000_2025.11.11.687878v1.1","unstructured":"Sonya Bells et al., \u201cTractometry Comprehensive Multimodal Quantitative Assessment of White Matter Along Specific Tracts,\u201d in Proceedings of the International Society for Magnetic Resonance in Medicine, May 2011."},{"key":"2025111623300845000_2025.11.11.687878v1.2","doi-asserted-by":"crossref","unstructured":"Yixue Feng et al., \u201cMicrostructural mapping of neural pathways in Alzheimer\u2019s disease using macrostructure-informed normative tractometry,\u201d Alzheimer\u2019s & Dementia, p. alz.14371, Dec. 2024.","DOI":"10.1101\/2024.04.25.591183"},{"key":"2025111623300845000_2025.11.11.687878v1.3","doi-asserted-by":"crossref","unstructured":"Bramsh Qamar Chandio et al., \u201cAmyloid, Tau, and APOE in Alzheimer\u2019s Disease: Impact on White Matter Tracts,\u201d Aug. 2024.","DOI":"10.1101\/2024.08.05.606560"},{"key":"2025111623300845000_2025.11.11.687878v1.4","unstructured":"Gaon S. 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