{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T23:54:17Z","timestamp":1781567657757,"version":"3.54.5"},"posted":{"date-parts":[[2025,11,17]]},"group-title":"Microbiology","reference-count":67,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The complex structure of fungal biofilms generates microenvironments that impact the fitness of cells within the biofilm community. Contributions to fitness include the development of emergent properties resulting in the tolerance or resistance to external stressors such as rapid environmental changes and in the context of an infection, antifungal drug exposure. The biofilm developed by the filamentous fungal pathogen\n                  <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                  develops zones of low oxygen which contribute to a reduction in antifungal drug susceptibility, however the genes and mechanisms involved in driving this emergent property of the biofilm are ill-defined. In this study, we utilized a transcriptomic approach to probe the biofilm structure in comparison to drug susceptible planktonic cultures to identify transcriptional patterns and genes unique to the\n                  <jats:italic>A. fumigatus<\/jats:italic>\n                  biofilm. Importantly we utilized two phenotypically diverse strains that allowed us to identify biofilm specific gene co-expression networks. One of these networks was highlighted by a gene encoding a ceramide synthase, designated\n                  <jats:italic>barA<\/jats:italic>\n                  , with a striking increase in\n                  <jats:italic>barA<\/jats:italic>\n                  transcript abundance specifically in the biofilm. Null mutants for\n                  <jats:italic>barA<\/jats:italic>\n                  in two strain backgrounds display altered biofilm morphology with some strain specific differences. Importantly,\n                  <jats:italic>barA<\/jats:italic>\n                  has a role in regulating susceptibility to ergosterol targeting antifungal drugs. These data identify biofilm specific genes in\n                  <jats:italic>A. fumigatus<\/jats:italic>\n                  for further study and highlight the importance of fungal ceramide synthases in mediating antifungal drug susceptibility in infection relevant biofilms.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>\n                    Biofilms are problematic structures in the context of microbial infections due to the ability to resist both host and drug mediated attempts at tissue sterilization. Consequently, it is imperative to identify mechanisms underlying the development of these structures and the emergent properties they develop. The filamentous fungal pathogen\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    forms robust structured biofilms that are resistant to contemporary antifungal drug treatments though the mechanisms are ill-defined. In this study we compared the transcriptional landscape of\n                    <jats:italic>two A.<\/jats:italic>\n                    fumigatus reference strains grown as biofilms and in planktonic culture conditions to identify biofilm specifc genes and pathways. These analyses and subsequent genetic and phenotype studies revealed that a ceramide synthase is important for the development of antifungal drug resistant biofilms. Consequently, these data support the rationale for targeting fungal lipid homeostasis for antifungal therapeutic development, particularly in the context of biofilm mediated infections.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.11.16.688756","type":"posted-content","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T19:50:15Z","timestamp":1763409015000},"source":"Crossref","is-referenced-by-count":2,"title":["A ceramide synthase is important for filamentous fungal biofilm morphology and antifungal drug resistance"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4402-0295","authenticated-orcid":false,"given":"Charles T.S.","family":"Puerner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owen M.","family":"Wilkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5503-5006","authenticated-orcid":false,"given":"Robert A.","family":"Cramer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025111907164971000_2025.11.16.688756v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.11.001"},{"key":"2025111907164971000_2025.11.16.688756v1.2","doi-asserted-by":"crossref","unstructured":"Katharios-Lanwermeyer S , O\u2019Toole GA . 2022. 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