{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T18:41:36Z","timestamp":1786819296247,"version":"build-2736575974"},"posted":{"date-parts":[[2025,11,25]]},"group-title":"Microbiology","reference-count":52,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015326","name":"NIH Common Fund","doi-asserted-by":"crossref","award":["R01 AI132606"],"award-info":[{"award-number":["R01 AI132606"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015326","name":"NIH Common Fund","doi-asserted-by":"crossref","award":["R01 AI153521"],"award-info":[{"award-number":["R01 AI153521"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,11,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Resistance to zoliflodacin, a first-in-class antibiotic for gonorrhea treatment, can occur through\n                  <jats:italic>gyrB<\/jats:italic>\n                  <jats:sup>D429N<\/jats:sup>\n                  , but this mutation\u2019s impact on fitness and resistance to other topoisomerase targeting drugs, including gepotidacin, have been unclear. Here, we show that\n                  <jats:italic>gyrB<\/jats:italic>\n                  <jats:sup>D429N<\/jats:sup>\n                  confers cross-resistance to gepotidacin in some clinical isolates and that its fitness effect varies with strain back-ground. These findings inform strategies for introducing the new topoisomerase inhibitors into clinical use and for surveillance of resistance.\n                <\/jats:p>","DOI":"10.1101\/2025.11.25.690462","type":"posted-content","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T02:40:17Z","timestamp":1764124817000},"source":"Crossref","is-referenced-by-count":5,"title":["Zoliflodacin and gepotidacin cross-resistance in\n                  <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-9945","authenticated-orcid":false,"given":"Aditi","family":"Mukherjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8967-3564","authenticated-orcid":false,"given":"Sofia OP","family":"Blomqvist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9319-7097","authenticated-orcid":false,"given":"David","family":"Helekal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1992-003X","authenticated-orcid":false,"given":"Apabrita A","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7849-8078","authenticated-orcid":false,"given":"Samantha G","family":"Palace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-1314","authenticated-orcid":false,"given":"Yonatan H","family":"Grad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025112700001054000_2025.11.25.690462v1.1","doi-asserted-by":"crossref","unstructured":"Gandra, S. et al. 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