{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:06:00Z","timestamp":1785434760306,"version":"3.56.0"},"posted":{"date-parts":[[2025,11,28]]},"group-title":"Biophysics","reference-count":12,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"Ovation Data Houston"},{"award":["R01GM133840"],"award-info":[{"award-number":["R01GM133840"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NSF","award":["CMMI1825941"],"award-info":[{"award-number":["CMMI1825941"]}]},{"name":"NAWI"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>The highly regulated assembly of different proteins forming mature P1 myophage capsid show a unique polymorphism where the virion population is primarily divided in brackets of two different capsid sizes. The contrasting capsids, sized 95nm (capsid_L), triangulation number (T)=13 and 68nm (capsid_S) with T=7, both in Dextro format and have the same protein forming the phage head with similar intra and inter capsomeric interactions. Comparative study of the electron density maps of the capsids reveals the presence of protein appendages DarA and Hdf below the 5-fold symmetric region inside capsid_L that anchor the phage dsDNA to the capsid-shell. Such densities are also noticed in large capsids depleted off their dsDNA but not observed in capsid_S. Deficiency of these appendages in capsid_S results in a smaller sized dsDNA packed inside capsid_S. Despite missing out on nearly 60% of the phage dsDNA virions with capsid_S has all the essential genes responsible for the formation of a fully mature thermodynamically stable virion particle with structurally identical tail and baseplate as of capsid_L. Co-existence of both stable conformation in a single lysate is a unique phenomenon in the phage community and suggest that DarA and Hdf play an important role in phage capsid morphogenesis.<\/jats:p>","DOI":"10.1101\/2025.11.26.689930","type":"posted-content","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T04:15:11Z","timestamp":1764389711000},"source":"Crossref","is-referenced-by-count":0,"title":["Structure of bacteriophage P1 head provides insight into capsid polymorphism"],"prefix":"10.64898","author":[{"given":"Anindito","family":"Sen","sequence":"first","affiliation":[{"name":"Microscopy and Imaging Center, Texas A&M University, College Station, Texas, 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsukasa","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana, 47907, USA"},{"name":"Structural Biology Research Center, Institute of Material Structure Science, High Energy Accelerator Research Organization, Tsukuba, Ibaraki, 3050801, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genki","family":"Tarashi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana, 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veer","family":"Bhatt","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific, 5350 NE Dawson Creek Drive. 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